Peptides / Longevity and mitochondria

Natural peptide · 24 amino acids · 2,687.2 Da

Humanin

Not approvedBanned in sport

Humanin is a 24-amino-acid peptide made from a gene in the mitochondria, studied for stress protection in lab cells. It is not approved and is banned in sport as an unapproved substance, and its studies are in animals and lab cells, mostly on brain cells. PepBank shows 1,063 results on humanin from animal and lab studies. No study in people has reported results yet. Data as of 2026-10-30.

Also known as HN, HNM, GTPL1047, HNGF6A, MT-RNR2, Colivelin and 3 more

24 amino acids, from first to last0 changed amino acids

Evidence level: Animal and lab studies onlyNo study in people has results. ClinicalTrials.gov lists no trial.

Results in people

0% of 1,067 results

Results in animals

527

49% of 1,067 results

Results in lab cells

536

50% of 1,067 results

From computer models

under 1% of 1,067 results

Overview

Humanin at a glance

What it is	Natural peptide, 24 amino acids
Approval	Not approved for any use
In sport	Banned in sport (WADA S0)
Studies	375 studies with results. 201 of them gave humanin, with 1,067 results. 527 in animals536 in lab cells4 from computer models
Clinical trials	None registered
Community use	Longevity and anti-aging; General vitality and mitochondrial support

The science

What is humanin?

Humanin is a 24-residue peptide made from a short reading frame inside the mitochondrial 16S rRNA gene. In laboratory studies it protects cells from stress and death signals. It is not an approved medicine.

Amino acids in one chain

Humanin structure and chemistry

The key facts about the molecule. Long codes are in the closed section below.

Sequence, 24 amino acidsFrom the first amino acid to the last

M1AP+RG5FSCLL10LLTS−E15I−DLPV20+K+R+RA

Darker means more water-repelling+ and − show charge in the body

Structure

Humanin is a natural peptide of 24 amino acids, with no ring, no changed amino acids.

Formula

C₁₁₉H₂₀₄N₃₄O₃₂S₂

Molecular weight

2,687.2 g/mol

CAS number

330936-69-1

Targets

N-formyl peptide receptor 2 (FPRL1) (gene FPR2): agonistEvidence: primary literatureN-formyl peptide receptor 3 (FPRL2) (gene FPR3): agonistEvidence: primary literatureCiliary neurotrophic factor receptor subunit alpha (part of the trimeric humanin receptor) (gene CNTFR): ligand that brings receptor subunits togetherEvidence: primary literature

Development

No company holds an approval or a registered treatment programme for humanin. The peptide was first described by Hashimoto Y, Nishimoto I and colleagues (PNAS 2001, PMID 11371646). Highest phase: preclinical.

Identifiers

PubChem 16131438, ChEMBL CHEMBL4447799, UNII H975EUX36G, KEGG C16068, IUPHAR 1047, UniProt Q8IVG9, PDB 1Y32

Full sequence, SMILES and InChIKey

Three-letter sequence
H-Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala-OH

InChIKey
DPEUWKZJZIPZKE-OFANTOPUSA-N

SMILES
CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](C)C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CO)NC(=O)[C@H](CC2=CC=CC=C2)NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H]3CCCN3C(=O)[C@H](C)NC(=O)[C@H](CCSC)N

See charge, composition and 3D structure on the Scientific page

The evidence

What humanin studies show

Results grouped by what the studies measured. Each card shows where its results come from.

Animal and lab studies onlyNo study in people has results. ClinicalTrials.gov lists no trial.

Brain and nerves

208 results · 93 animal · 115 lab

In mice with induced stroke, HNG given before the stroke reduced the size of the brain damage. Stroke, 2006

Top results

In mice with induced stroke, HNG given before the stroke reduced the size of the brain damage. (Stroke, 2006)
In an animal model of retinal injury, humanin kept retinal function from declining. (Aging cell, 2026)
In cells, a humanin analogue protected several cell types from stress-induced death. (The Journal of cell biology, 2018)

Immune system and inflammation

136 results · 83 animal · 53 lab

In mice on a high-cholesterol diet, humanin lowered a kidney inflammation marker. Life sciences, 2012

Top results

In mice on a high-cholesterol diet, humanin lowered a kidney inflammation marker. (Life sciences, 2012)
In mice with asthma, a humanin analog reduced lung weight increase compared with untreated asthmatic mice. (Aging, 2023)
In mice with induced stroke, a humanin analogue lowered TNF-alpha. (IUBMB life, 2018)

Cells, genes and aging

182 results · 65 animal · 117 lab

In diabetic mice, humanin raised antioxidant levels in seminal fluid compared with untreated diabetic mice. Experimental physiology, 2026

Top results

In diabetic mice, humanin raised antioxidant levels in seminal fluid compared with untreated diabetic mice. (Experimental physiology, 2026)
In mice with induced ovarian insufficiency, a humanin derivative reduced cell death in ovarian support cells. (Reproductive biomedicine online, 2024)
In mice, peptide treatment was linked to better-looking mitochondria in heart atria. (Biomedicines, 2026)

Sex hormones and fertility

81 results · 50 animal · 31 lab

In diabetic mice, humanin reduced testicular tissue damage such as tubule degeneration and germ cell loss. Experimental physiology, 2026

Top results

In diabetic mice, humanin reduced testicular tissue damage such as tubule degeneration and germ cell loss. (Experimental physiology, 2026)
In diabetic mice, S14G-humanin raised the share of live fetuses. (Gynecological endocrinology, 2022)
In male rats given paroxetine, humanin restored the balance of reproductive hormones. (Reproductive biology, 2026)

Heart and blood vessels

68 results · 49 animal · 19 lab

In mice, a humanin analogue made atrial fibrillation less easy to trigger. Biomedicines, 2026

Top results

In mice, a humanin analogue made atrial fibrillation less easy to trigger. (Biomedicines, 2026)
In mice, HNG reduced blood clot formation in an injured artery compared with saline. (Journal of cellular and molecular medicine, 2020)
In mice with hemorrhagic shock, humanin-G improved blood pressure compared with vehicle. (Shock, 2023)

Memory and thinking

26 results · 24 animal · 2 lab

In mice, a more potent humanin form reversed the short-term memory impairment caused by scopolamine. British journal of pharmacology, 2001

Top results

In mice, a more potent humanin form reversed the short-term memory impairment caused by scopolamine. (British journal of pharmacology, 2001)
In mice with induced memory loss, S14G-HN prevented a drop in short-term memory in a maze test. (Journal of neuroscience research, 2005)
In aged mice, humanin improved cognition. (Scientific reports, 2018)

All 375 humanin studies by area Open the Scientific page

Key humanin studies

Studies in people come first, then studies in animals and in the lab.

Animals
Humanin and MOTS-c Attenuate Atrial Fibrillation by Suppressing Fibrosis and Mitochondrial Dysfunction
Liao et al. 2026 · Biomedicines · 36 subjects · cited by 0
10 results
Animals
Humanin prevents intra-renal microvascular remodeling and inflammation in hypercholesterolemic ApoE deficient mice
Zhang et al. 2012 · Life sciences · 24 subjects · cited by 54
11 results
Animals
Humanin ameliorates diabetes-induced testicular damage in a streptozotocin-induced mouse model
Hekim et al. 2026 · Experimental physiology · 20 subjects · cited by 1
5 results
Animals
Humanin Restores Metabolic Hormone Homeostasis of Leptin, Ghrelin, Irisin and Asprosin in Streptozotocin-Induced Diabetic Mice
Bulut et al. 2026 · Metabolites · 20 subjects · cited by 1
4 results
Animals
Humanin peptide ameliorates reproductive dysfunction and restores neuroendocrine mechanisms in SSRI-treated male rats
Köm et al. 2026 · Reproductive biology · 20 subjects · cited by 0
6 results

All 375 Humanin studies

Humanin clinical trials

ClinicalTrials.gov lists no trial of humanin. A trial under another name may exist.

Status and safety

Is humanin approved?

FDA

United States

Not approved

No approved medicine contains it.

EMA

European Union

Not approved

No approved medicine contains it.

WADA

Sport

Banned

On the 2026 Prohibited List, section S0.

No. Not approved for any use. Preclinical research only. No clinical trial phase found. The FDA, the EMA and the other sources we use show no approval (as of 13 Oct 2026). Banned in sport (WADA S0).

Laws and labels change, so check the regulator for the current status.

Humanin side effects and safety

PepBank has 6 safety results from studies of humanin. None of them come from studies in people, so they do not show what happens in people.

Safety results from studies (6)

In mice without doxorubicin, a humanin analogue had no harmful effect on the heart. (American journal of physiology. Heart and circulatory physiology, 2018)
In mice, HNG did not make bleeding time longer than saline. (Journal of cellular and molecular medicine, 2020)
In mice, the humanin derivative S14G-HN did not change movement in either test. (Neuropeptides, 2017)
In mice, HNG did not change physical activity. (Biochimica et biophysica acta. General subjects, 2022)
In mice, adding HNG to glucocorticoids did not make muscle damage worse. (Biochemistry and biophysics reports, 2026)
In cells, a Humanin analogue (HNG) did not cause abnormal cell growth. (Reproductive biomedicine online, 2025)

See every safety result on the Scientific page

Doses studied (40 shown)

The doses that these studies used. They are not recommendations.

Dose as reported	Class	Species	Route	Duration	Design	Source
4 mg/kg/day	Animal	mouse	intraperitoneal	16 weeks	Study in live animals · n = 24	Zhang 2012
4 mg/kg	Animal	mouse	intraperitoneal	15 days	Study in live animals · n = 20	Hekim 2026
4 mg/kg/day	Animal	mouse	intraperitoneal	15 days	Study in live animals · n = 20	Bulut 2026
1 mg/kg/day	Animal	rat	subcutaneous infusion	Not reported	Study in live animals · n = 20	Köm 2026
4 mg/kg	Animal	mouse	Not reported	15 consecutive days	Study in live animals · n = 20	Bulut 2026
4 mg/kg 2 times/wk	Animal	mouse	intraperitoneal	14 months	Study in live animals · n = 14	Qin 2018
252 μg/kg	Animal	rat	intraperitoneal	Not reported	Study in live animals · n = 14	Onat 2023
4 mg/kg	Animal	mouse	Not reported	15 days	Study in live animals · n = 10	Kelestemur 2024
2.5 mg/kg/injection twice a day	Animal	mouse	intraperitoneal injection	3 days	Study in live animals · n = 6	Mehta 2019
0.1 microg	Animal	mouse	intracerebroventricular	Not reported	Study in live animals	Xu 2006
1 microg	Animal	mouse	intraperitoneal	Not reported	Study in live animals	Xu 2006
2 mg/kg (maximal reduction)	Animal	mouse	intraperitoneal	Not reported	Study in live animals	Muzumdar 2010
1000 pmol per 5 microl	Animal	mouse	intracerebroventricular	Not reported	Study in live animals	Mamiya 2001
at least 50 pmol	Animal	mouse	intracerebroventricular	Not reported	Study in live animals	Tajima 2005
252 μg/kg	Animal	rat	intravenous	Not reported	Study in live animals	Thummasorn 2017
1 µg/mouse	Animal	mouse	Not reported	one 2-week cycle (2 injections/week)	Study in live animals	Eriksson 2014
2.5 μg in 100 μl saline	Animal	mouse	intraperitoneal	Not reported	Study in live animals	Wang 2013
84, 168 and 252 ug/kg HNG	Animal	rat	intravenous	Not reported	Study in live animals	Kumfu 2018
5 mg/kg daily	Animal	mouse	intraperitoneal	10 weeks	Study in live animals	Lue 2018
84 μg kg(-1)	Animal	rat	intravenous	Not reported	Study in live animals	Kumfu 2016
2 mg/kg	Animal	pig	Not reported	Not reported	Study in live animals	Sharp 2020
4 mg/kg	Animal	mouse	Not reported	15 days	Study in live animals	Bulut 2023
100 μg/kg/day	Animal	mice	Not reported	14 days	Study in live animals	Celvin 2020
252 μg/kg	Animal	rat	intraperitoneal	Not reported	Study in live animals	Abozaid 2023
0.4 mg/Kg/day	Animal	rat	intraperitoneal	sixteen weeks	Study in live animals	Moin 2023
10 mg/kg	Animal	mouse	intraperitoneal	6 weeks	Study in live animals	Huang 2024
2 mg/kg	Animal	rat	intraperitoneal	Not reported	Study in live animals	Onat 2023
10 and 20 μM	Animal	rat	intraperitoneal	7 days (doses on day 2 and day 6)	Study in live animals	Gultekin 2017
4 mg/kg	Animal	mouse	intraperitoneal	15 days	Study in live animals	Bilgin 2025
2.5 mg/kg and 5 mg/kg	Animal	mouse	Not reported	16 days	Study in live animals	Su 2023
0.01, 0.05, 0.1 and 1 nmol	Animal	rat	Not reported	from day 4 to day 14	Study in live animals	Negintaji 2015
2 mg/kg	Animal	rat	intravenous	Not reported	Study in live animals	Abueid 2026
2.5 or 5 mg/kg/day	Animal	mouse	intraperitoneal	5 days before hvKP challenge	Study in live animals	Zhong 2026
5 mg/kg/day	Animal	mouse	intraperitoneal	5 days before hvKP challenge	Study in live animals	Zhong 2026
5 and 10 µM	Lab	buffalo	added to semen extender	Not reported	Study on tissue taken from a body · n = 24	Katiyar 2022
5 µM	Lab	buffalo	added to semen extender	Not reported	Study on tissue taken from a body · n = 24	Katiyar 2022
2.5, 5 and 7.5 µM	Lab	bovine	added to semen extender	Not reported	Study on tissue taken from a body · n = 24	Gemeda 2026
5 µM	Lab	bovine	added to semen extender	Not reported	Study on tissue taken from a body · n = 24	Gemeda 2026
2, 5 and 10 µM	Lab	buffalo	added to semen extender	Not reported	Study on tissue taken from a body · n = 18	Katiyar 2022
5 and 10 µM	Lab	buffalo	added to semen extender	Not reported	Study on tissue taken from a body · n = 18	Katiyar 2022

From the community

How people use it

What people report online. It comes from forums and guides, not from studies.

Community-reported. Not tested in trials.

Few people report using humanin. The sources describe small groups who inject it under the skin for longevity and mitochondrial support, mostly as the stronger S14G analog (HNG) sold under the name humanin. The amounts in the sources differ by a factor of ten or more, and no source describes a trial.

Reported routines and cautions

What people use it for

Longevity and anti-agingS1S2S5
General vitality and mitochondrial supportS2

Reported regimens

Regimens that community sources report for Humanin. Community-reported, not tested in trials.
Context	Route	Amount	How often	How long	Agreement	Sources
Self-experiment with an S14G variant bought from a custom peptide maker (one poster)	subcutaneous injection	0.4 to 1 mg per dose	Not stated	Not stated	single source; the site carries ads (affiliate)	S1
One poster's round of 'Humanin' from a supplier the poster calls a quality source	subcutaneous injection	200 mcg per dose	Not stated	Not stated	single source; the site carries ads (affiliate)	S1
First injection reported by a forum member after buying a 10 mg vial	subcutaneous injectionloaded into a pen after mixing with bacteriostatic water	500 mcg per dose	Not stated	Not stated	single source; the site carries seller links (affiliate)	S4
Compiled 'standard HNG analog protocol' on a community wiki	subcutaneous injection	1 to 5 mg per dose	2 to 3 times weekly	4 to 8 weeks on, then 2 to 4 weeks off	single source; the wiki carries supplier rankings (affiliate); it compiles other pages and does not cite them	S2
Dosing summary on a forum-run reference page, written for the HNG analog	subcutaneous injection	0.5 to 2 mg per dose	3 times per week	4 to 8 weeks	sources differ: 0.5 to 2 mg per dose (S3) against 1 to 5 mg per dose (R4, S2) against 0.4 to 1 mg (R1) and 0.2 to 0.5 mg (R2, R3). Each is a single source and none is an independent set of many reports.	S3

Reported side effects

swollen bags under the eyes the morning after a 200 mcg injection, still present two months later (one poster, 2021); a second poster, who had earlier reported no problems at 0.4 to 1 mg of an S14G variant (R1), wrote in 2022 that bags appeared at about the time of their own injectionsS1
injection-site redness or irritationS3

Reported benefits (claims, not shown in trials)

no problems after injections of 0.4 to 1 mg of an S14G variant (one poster, 2017; the same poster later recalled eye bags, see above)S1
improved overall sense of vitality and well-being (reported by 'some users', not counted)S2
generally well tolerated with minimal side effects (wiki summary, not counted)S2

Cautions

No human trial of injected humanin or HNG exists, so every amount comes from other users or from animal work.S3S2
Native humanin and HNG are sold under the same name and are not equally strong. One source says HNG is about 1000 times more potent, and that vendor labels do not always say which one is in the vial.S2S3
People in the forum threads ask what a human dose is and say they have seen no studies in people.S1S5

Status notes

FDA: humanin is not named on the 503A categories list (updated 2026-05-14), the 503B categories list (updated 2025-03-21) or the FDA category 2 safety page. It has no Drugs@FDA record and is not an approved medicine.S6 S7 S8
WADA: humanin is not named in the 2026 or 2027 Prohibited List. The S0 class (non-approved substances) is prohibited at all times and covers a substance that no other section addresses and that has no regulatory approval for human therapeutic use, so S0 applies by its wording. This is a reading of the List text. WADA has not said so by name.S9 S10

Community sources

S1Humanin - Supplements (forum thread, 2017 to 2022)longecity.orgcarries seller links, not linked
S2Humanin Community Protocols & Anecdotal Reportspeptideprotocolwiki.comcarries seller links, not linked
S3Humanin: Dosage, Half-Life & Reconstitution (opens in a new tab)jackedforums.com
S4Humanin (forum thread, 2026)community.peptidecritic.comcarries seller links, not linked
S5The mitochondrial derived peptide humanin is a regulator of lifespan and healthspan - Newslongecity.orgcarries seller links, not linked
S6Certain bulk drug substances for use in compounding that may present significant safety risks (opens in a new tab)fda.gov
S7FDA 503A categories of bulk drug substances (updated 2026-05-14) (opens in a new tab)fda.gov
S8FDA 503B categories of bulk drug substances (updated 2025-03-21) (opens in a new tab)fda.gov
S9WADA Prohibited List 2026 (opens in a new tab)wada-ama.org
S10WADA Prohibited List 2027 (opens in a new tab)wada-ama.org

Gaps

Community use of humanin is thin. Searches found a few forum threads and compiled wiki pages. Only 3 first-person amount reports could be quoted (S1 twice, S4 once). No source gives a frequency or a length of use for a first-person report. S2 and S3 are compiled pages that do not cite individual posts, so R4 and R5 are not independent reports. S2 and S4 carry seller links or rankings (affiliate), and S4 links to sellers inside the thread. The Longecity threads (S1, S5) show Google ads and sponsor slots but no seller ranking, and S5 posts link to sellers. S3 is an editorial dosing page of a forum site (its shop page says it is not open yet), not a set of user posts. Two more threads were found and not used: PepAtlas forum threads (pepatlas.com/forum/optimization/cmo28u5qz0hjj4ekecg5diaex and pepatlas.com/forum/longevity-peptides/cmo29wscc0a4d2tp9ywv1e43u) contain reports such as 5 mg twice a week for 6 weeks and 1 mg three times a week for 8 weeks. The quote checker could not open the site (certificate error), so those reports were not written as regimens. A GLP-1 Forum thread asks about humanin and gives no dose. The YouTube video in the candidate list (sWrBduR7sis) was not checked because a video transcript cannot be quoted by the checker. Seller and calculator pages (for example dosagepeptide.com, peptidedosing.info, optipin.app) give 0.5 to 5 mg two to three times weekly or daily. They are vendor or affiliate pages with no named user and were not used. Most sources do not say whether the product was native humanin or HNG. No source reports lab tests, purity checks or a certificate of analysis for the product used. No Reddit pages were used.

Questions and more

Ask a question about humanin

The answer links to every study it uses.

What does the evidence say about humanin?Is humanin tested in people?Compare with AICAR (acadesine)Compare with NMN (nicotinamide mononucleotide)

Sources

Database: PepBank data for humanin (data version 308560)
Regulator: FDA status record
Regulator: EMA status record
Regulator: WADA status record
Community: community sources (forums and guides), as summarized in the PepBank community file

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

PepBank is a research reference. Talk to a doctor before you use any peptide.

Ask your AI about humanin

Curo is a peptide shop. PepBank and Curo have the same owner. See the Curo catalog (opens in a new tab).

Humanin

Not approvedBanned in sport

Also known as HN, HNM, GTPL1047, HNGF6A, MT-RNR2, Colivelin and 3 more

What it is

Natural peptide, 24 amino acids

Approval

Not approved for any use

In sport

Banned in sport (WADA S0)

Studies

375 studies with results. 201 of them gave humanin, with 1,067 results.
527 in animals536 in lab cells4 from computer models

Clinical trials

None registered

Community use

Longevity and anti-aging; General vitality and mitochondrial support

Dose as reported

Class

Species

Route

Duration

Design

Source

4 mg/kg/day

Animal

mouse

intraperitoneal

16 weeks

Study in live animals · n = 24

Zhang 2012

4 mg/kg

Animal

mouse

intraperitoneal

15 days

Study in live animals · n = 20

Hekim 2026

4 mg/kg/day

Animal

mouse

intraperitoneal

15 days

Study in live animals · n = 20

Bulut 2026

1 mg/kg/day

Animal

rat

subcutaneous infusion

Not reported

Study in live animals · n = 20

Köm 2026

4 mg/kg

Animal

mouse

Not reported

15 consecutive days

Study in live animals · n = 20

Bulut 2026

4 mg/kg 2 times/wk

Animal

mouse

intraperitoneal

14 months

Study in live animals · n = 14

Qin 2018

252 μg/kg

Animal

rat

intraperitoneal

Not reported

Study in live animals · n = 14

Onat 2023

4 mg/kg

Animal

mouse

Not reported

15 days

Study in live animals · n = 10

Kelestemur 2024

2.5 mg/kg/injection twice a day

Animal

mouse

intraperitoneal injection

3 days

Study in live animals · n = 6

Mehta 2019

0.1 microg

Animal

mouse

intracerebroventricular

Not reported

Study in live animals

Xu 2006

1 microg

Animal

mouse

intraperitoneal

Not reported

Study in live animals

Xu 2006

2 mg/kg (maximal reduction)

Animal

mouse

intraperitoneal

Not reported

Study in live animals

Muzumdar 2010

1000 pmol per 5 microl

Animal

mouse

intracerebroventricular

Not reported

Study in live animals

Mamiya 2001

at least 50 pmol

Animal

mouse

intracerebroventricular

Not reported

Study in live animals

Tajima 2005

252 μg/kg

Animal

rat

intravenous

Not reported

Study in live animals

Thummasorn 2017

1 µg/mouse

Animal

mouse

Not reported

one 2-week cycle (2 injections/week)

Study in live animals

Eriksson 2014

2.5 μg in 100 μl saline

Animal

mouse

intraperitoneal

Not reported

Study in live animals

Wang 2013

84, 168 and 252 ug/kg HNG

Animal

rat

intravenous

Not reported

Study in live animals

Kumfu 2018

5 mg/kg daily

Animal

mouse

intraperitoneal

10 weeks

Study in live animals

Lue 2018

84 μg kg(-1)

Animal

rat

intravenous

Not reported

Study in live animals

Kumfu 2016

2 mg/kg

Animal

pig

Not reported

Study in live animals

Sharp 2020

4 mg/kg

Animal

mouse

Not reported

15 days

Study in live animals

Bulut 2023

100 μg/kg/day

Animal

mice

Not reported

14 days

Study in live animals

Celvin 2020

252 μg/kg

Animal

rat

intraperitoneal

Not reported

Study in live animals

Abozaid 2023

0.4 mg/Kg/day

Animal

rat

intraperitoneal

sixteen weeks

Study in live animals

Moin 2023

10 mg/kg

Animal

mouse

intraperitoneal

6 weeks

Study in live animals

Huang 2024

2 mg/kg

Animal

rat

intraperitoneal

Not reported

Study in live animals

Onat 2023

10 and 20 μM

Animal

rat

intraperitoneal

7 days (doses on day 2 and day 6)

Study in live animals

Gultekin 2017

4 mg/kg

Animal

mouse

intraperitoneal

15 days

Study in live animals

Bilgin 2025

2.5 mg/kg and 5 mg/kg

Animal

mouse

Not reported

16 days

Study in live animals

Su 2023

0.01, 0.05, 0.1 and 1 nmol

Animal

rat

Not reported

from day 4 to day 14

Study in live animals

Negintaji 2015

2 mg/kg

Animal

rat

intravenous

Not reported

Study in live animals

Abueid 2026

2.5 or 5 mg/kg/day

Animal

mouse

intraperitoneal

5 days before hvKP challenge

Study in live animals

Zhong 2026

5 mg/kg/day

Animal

mouse

intraperitoneal

5 days before hvKP challenge

Study in live animals

Zhong 2026

5 and 10 µM

Lab

buffalo

added to semen extender

Not reported

Study on tissue taken from a body · n = 24

Katiyar 2022

5 µM

Lab

buffalo

added to semen extender

Not reported

Study on tissue taken from a body · n = 24

Katiyar 2022

2.5, 5 and 7.5 µM

Lab

bovine

added to semen extender

Not reported

Study on tissue taken from a body · n = 24

Gemeda 2026

5 µM

Lab

bovine

added to semen extender

Not reported

Study on tissue taken from a body · n = 24

Gemeda 2026

2, 5 and 10 µM

Lab

buffalo

added to semen extender

Not reported

Study on tissue taken from a body · n = 18

Katiyar 2022

5 and 10 µM

Lab

buffalo

added to semen extender

Not reported

Study on tissue taken from a body · n = 18

Katiyar 2022

Context

Route

Amount

How often

How long

Agreement

Sources

Self-experiment with an S14G variant bought from a custom peptide maker (one poster)

subcutaneous injection

0.4 to 1 mg per dose

Not stated

single source; the site carries ads (affiliate)

One poster's round of 'Humanin' from a supplier the poster calls a quality source

subcutaneous injection

200 mcg per dose

Not stated

single source; the site carries ads (affiliate)

First injection reported by a forum member after buying a 10 mg vial

subcutaneous injectionloaded into a pen after mixing with bacteriostatic water

500 mcg per dose

Not stated

single source; the site carries seller links (affiliate)

Compiled 'standard HNG analog protocol' on a community wiki

subcutaneous injection

1 to 5 mg per dose

2 to 3 times weekly

4 to 8 weeks on, then 2 to 4 weeks off

single source; the wiki carries supplier rankings (affiliate); it compiles other pages and does not cite them

Dosing summary on a forum-run reference page, written for the HNG analog

subcutaneous injection

0.5 to 2 mg per dose

3 times per week

4 to 8 weeks

sources differ: 0.5 to 2 mg per dose (S3) against 1 to 5 mg per dose (R4, S2) against 0.4 to 1 mg (R1) and 0.2 to 0.5 mg (R2, R3). Each is a single source and none is an independent set of many reports.

Humanin

Humanin at a glance

What is humanin?

Humanin structure and chemistry

What humanin studies show

Brain and nerves

Immune system and inflammation

Cells, genes and aging

Sex hormones and fertility

Heart and blood vessels

Memory and thinking

Key humanin studies

Humanin clinical trials

Is humanin approved?

Humanin side effects and safety

How people use it

What people use it for

Reported regimens

Reported side effects

Reported benefits (claims, not shown in trials)

Cautions

Status notes

Community sources

Ask a question about humanin

Related peptides

Sources

Humanin

Humanin at a glance

What is humanin?

Humanin structure and chemistry

What humanin studies show

Brain and nerves

Immune system and inflammation

Cells, genes and aging

Sex hormones and fertility

Heart and blood vessels

Memory and thinking

Key humanin studies

Humanin clinical trials

Is humanin approved?

Humanin side effects and safety

How people use it

What people use it for

Reported regimens

Reported side effects

Reported benefits (claims, not shown in trials)

Cautions

Status notes

Community sources

Ask a question about humanin

Related peptides

Sources