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Study · Animal study (in vivo) · mouse · 2018

8 results

Chronic treatment with the mitochondrial peptide humanin prevents age-related myocardial fibrosis in mice.

Qin Q, Mehta H, Yen K, Navarrete G, Brandhorst S, Wan J, Delrio S, Zhang X, Lerman LO, Cohen P, Lerman A

American journal of physiology. Heart and circulatory physiology13 Jul 2018Cited by 53 (Europe PMC)

PMID30004252 (opens in a new tab)PMCIDPMC6415743 (opens in a new tab)DOI10.1152/ajpheart.00685.2017 (opens in a new tab)PepBankPUB-30004252
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In aged female mice, HNG treatment significantly increased the ratio of cardiomyocytes to fibroblasts in the heart.

    In plain wordsIn aged mice, a humanin analogue raised the ratio of heart muscle cells to fibroblasts compared with vehicle.

    Peptide
    Humanin
    Outcome
    IncreaseRatio of cardiomyocytes to fibroblasts
    Study

    mousen = 14

    Aged C57BL/6N female mice
    Comparator
    Vehicle-treated old mice
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    4 mg/kg 2 times/wk · intraperitoneal · 14 monthsAs given in this study (mouse).

    Source sentences

    Summary
    HNG treatment significantly increased the ratio of cardiomyocytes to fibroblasts in aging hearts, as shown by the percentage of each cell type in randomly chosen fields after immunofluorescence staining.
    1 context sentence
    Summary · context
    Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In aged female mice, HNG treatment significantly reduced collagen deposition in the heart.

    In plain wordsIn aged mice, a humanin analogue reduced collagen build-up in the heart compared with vehicle.

    Peptide
    Humanin
    Outcome
    DecreaseCollagen deposition in aged hearts
    Study

    mousen = 14

    Aged C57BL/6N female mice
    Comparator
    Vehicle-treated old mice
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    4 mg/kg 2 times/wk · intraperitoneal · 14 monthsAs given in this study (mouse).

    Source sentences

    Summary
    Furthermore, the increased collagen deposition in aged hearts was significantly reduced after HNG treatment, as indicated by picrosirius red staining.
    1 context sentence
    Summary · context
    Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In aged female mice, HNG treatment reduced cardiac fibroblast proliferation.

    In plain wordsIn aged mice, a humanin analogue slowed heart fibroblast growth compared with vehicle.

    Peptide
    Humanin
    Outcome
    DecreaseCardiac fibroblast proliferation
    Study

    mousen = 14

    Aged C57BL/6N female mice
    Comparator
    Vehicle-treated old mice
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    4 mg/kg 2 times/wk · intraperitoneal · 14 monthsAs given in this study (mouse).

    Source sentences

    Summary
    HNG treatment also reduced in aging mice cardiac fibroblast proliferation (5'-bromo-2-deoxyuridine staining) and attenuated transforming growth factor-β1, fibroblast growth factor-2, and matrix metalloproteinase-2 expression (immunohistochemistry or real-time PCR).
    1 context sentence
    Summary · context
    Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In aged female mice, HNG treatment attenuated transforming growth factor-beta1 expression.

    In plain wordsIn aged mice, a humanin analogue lowered a fibrosis signal, TGF-beta1, in the heart.

    Peptide
    Humanin
    Outcome
    DecreaseTGF-beta1 expression
    Study

    mousen = 14

    Aged C57BL/6N female mice
    Comparator
    Vehicle-treated old mice
    Outcome group
    Inflammation markers · Cardiovascular
    Studied dose
    4 mg/kg 2 times/wk · intraperitoneal · 14 monthsAs given in this study (mouse).

    Source sentences

    Summary
    HNG treatment also reduced in aging mice cardiac fibroblast proliferation (5'-bromo-2-deoxyuridine staining) and attenuated transforming growth factor-β1, fibroblast growth factor-2, and matrix metalloproteinase-2 expression (immunohistochemistry or real-time PCR).
    1 context sentence
    Summary · context
    Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In aged female mice, HNG treatment attenuated fibroblast growth factor-2 expression.

    In plain wordsIn aged mice, a humanin analogue lowered fibroblast growth factor-2 in the heart.

    Peptide
    Humanin
    Outcome
    DecreaseFGF-2 expression
    Study

    mousen = 14

    Aged C57BL/6N female mice
    Comparator
    Vehicle-treated old mice
    Outcome group
    Inflammation markers · Cardiovascular
    Studied dose
    4 mg/kg 2 times/wk · intraperitoneal · 14 monthsAs given in this study (mouse).

    Source sentences

    Summary
    HNG treatment also reduced in aging mice cardiac fibroblast proliferation (5'-bromo-2-deoxyuridine staining) and attenuated transforming growth factor-β1, fibroblast growth factor-2, and matrix metalloproteinase-2 expression (immunohistochemistry or real-time PCR).
    1 context sentence
    Summary · context
    Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In aged female mice, HNG treatment attenuated matrix metalloproteinase-2 expression.

    In plain wordsIn aged mice, a humanin analogue lowered matrix metalloproteinase-2 in the heart.

    Peptide
    Humanin
    Outcome
    DecreaseMMP-2 expression
    Study

    mousen = 14

    Aged C57BL/6N female mice
    Comparator
    Vehicle-treated old mice
    Outcome group
    Inflammation markers · Cardiovascular
    Studied dose
    4 mg/kg 2 times/wk · intraperitoneal · 14 monthsAs given in this study (mouse).

    Source sentences

    Summary
    HNG treatment also reduced in aging mice cardiac fibroblast proliferation (5'-bromo-2-deoxyuridine staining) and attenuated transforming growth factor-β1, fibroblast growth factor-2, and matrix metalloproteinase-2 expression (immunohistochemistry or real-time PCR).
    1 context sentence
    Summary · context
    Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age.
  7. 07AnimalAnimal study (in vivo)Efficacy outcome

    In aged female mice, HNG treatment inhibited myocardial apoptosis.

    In plain wordsIn aged mice, a humanin analogue reduced heart cell death compared with vehicle.

    Peptide
    Humanin
    Outcome
    DecreaseMyocardial apoptosis
    Study

    mousen = 14

    Aged C57BL/6N female mice
    Comparator
    Vehicle-treated old mice
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    4 mg/kg 2 times/wk · intraperitoneal · 14 monthsAs given in this study (mouse).

    Source sentences

    Summary
    Myocardial apoptosis was inhibited in HNG-treated aged mice (TUNEL staining).
    1 context sentence
    Summary · context
    Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In aged female mice, HNG upregulated the Akt/glycogen synthase kinase-3beta pathway.

    In plain wordsIn aged mice, a humanin analogue switched on the Akt/GSK-3beta pathway in the heart.

    Peptide
    Humanin
    Outcome
    IncreaseAkt/GSK-3beta pathway
    Study

    mousen = 14

    Aged C57BL/6N female mice
    Comparator
    Vehicle-treated old mice
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    4 mg/kg 2 times/wk · intraperitoneal · 14 monthsAs given in this study (mouse).

    Source sentences

    Summary
    To decipher the pathway involved in the attenuation of the myocardial fibrosis by HNG, Western blot analysis was done and showed that HNG upregulated the Akt/glycogen synthase kinase -3β pathway in aged mice.
    1 context sentence
    Summary · context
    Female C57BL/6N mice at 18 mo of age received 14-mo intraperitoneal injections of vehicle (old group; n = 6) or HN analog (HNG; 4 mg/kg 2 times/wk, old + HNG group, n = 8) and were euthanized at 32 mo of age.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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