The protective effects of humanin in rats with experimental myocardial infarction: The role of asprosin and spexin.

Onat E, Kocaman N, Hancer S

Heliyon26 Jul 2023Cited by 8 (Europe PMC)

PMID37576267PMCIDPMC10412837DOI10.1016/j.heliyon.2023.e18739PepBankPUB-37576267

Summary

ObjectiveIn this study, by applying humanin (HN) before myocardial infarction (MI) in rats, its protection in MI and the possible roles in asprosin and spexin were investigated.Materials and methodsThe rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day). To create MI, 200 mg/kg isoproterenol (ISO) was administered subcutaneously to the rats. 2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI. Molecular parameters asprosin and spexin were examined by immunohistochemical in heart tissue. Biochemical parameters (aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase MB (CK-MB), Troponin I) were studied in serum by enzyme-linked immunosorbent assay (ELISA) method.ResultsIt was found in the study that the levels of spexin elevated especially towards the 7th day after MI and decreased more significantly towards the 7th day, after HN application before MI. Asprosin elevated significantly towards the 7th day after MI and decreased especially on the 7th day after HN application before MI. Also, serum AST, LDH, CK-MB, and Troponin I levels tended to decrease and a significant decrease was detected in congested veins in the heart tissue at the 48th hour of MI and erythrocyte extravasation, congested veins and necrotic muscle fibers at the 7th day of MI in rats given HN before MI.ConclusionIt was concluded that HN has a cardioprotective effect in MI and asprosin and spexin might mediate this effect.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, humanin given before infarction decreased spexin in heart tissue more clearly towards day 7.
In plain wordsIn rats with a heart attack model, humanin given beforehand lowered a heart tissue signal (spexin) compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseHeart spexin on day 7 after MI
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
It was found in the study that the levels of spexin elevated especially towards the 7th day after MI and decreased more significantly towards the 7th day, after HN application before MI.
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626701
02AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, humanin given before infarction decreased asprosin in heart tissue especially on day 7.
In plain wordsIn rats with a heart attack model, humanin given beforehand lowered a heart tissue signal (asprosin) compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseHeart asprosin on day 7 after MI
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Asprosin elevated significantly towards the 7th day after MI and decreased especially on the 7th day after HN application before MI.
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626702
03AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, serum AST tended to decrease with humanin given before infarction.
In plain wordsIn rats with a heart attack model, a heart injury marker (AST) tended to fall with humanin compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseSerum AST
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Also, serum AST, LDH, CK-MB, and Troponin I levels tended to decrease
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626703
04AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, serum LDH tended to decrease with humanin given before infarction.
In plain wordsIn rats with a heart attack model, a heart injury marker (LDH) tended to fall with humanin compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseSerum LDH
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Also, serum AST, LDH, CK-MB, and Troponin I levels tended to decrease
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626704
05AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, serum CK-MB tended to decrease with humanin given before infarction.
In plain wordsIn rats with a heart attack model, a heart injury marker (CK-MB) tended to fall with humanin compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseSerum CK-MB
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Also, serum AST, LDH, CK-MB, and Troponin I levels tended to decrease
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626705
06AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, serum troponin I tended to decrease with humanin given before infarction.
In plain wordsIn rats with a heart attack model, a heart injury marker (troponin I) tended to fall with humanin compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseSerum troponin I
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Also, serum AST, LDH, CK-MB, and Troponin I levels tended to decrease
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626706
07AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, humanin given before infarction significantly decreased congested veins in heart tissue at the 48th hour.
In plain wordsIn rats with a heart attack model, humanin given beforehand reduced vein congestion in the heart at 48 hours compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseCongested veins in heart tissue at 48 hours
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
a significant decrease was detected in congested veins in the heart tissue at the 48th hour of MI and erythrocyte extravasation, congested veins and necrotic muscle fibers at the 7th day of MI in rats given HN before MI.
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626707
08AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, humanin given before infarction significantly decreased erythrocyte extravasation in heart tissue at day 7.
In plain wordsIn rats with a heart attack model, humanin given beforehand reduced blood cell leakage in the heart at day 7 compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseHeart erythrocyte extravasation at day 7
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
erythrocyte extravasation, congested veins and necrotic muscle fibers at the 7th day of MI in rats given HN before MI.
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626708
09AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, humanin given before infarction significantly decreased congested veins in heart tissue at day 7.
In plain wordsIn rats with a heart attack model, humanin given beforehand reduced vein congestion in the heart at day 7 compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseCongested veins in heart tissue at day 7
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
erythrocyte extravasation, congested veins and necrotic muscle fibers at the 7th day of MI in rats given HN before MI.
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626709
10AnimalAnimal study (in vivo)Efficacy outcome
In rats with isoproterenol-induced myocardial infarction, humanin given before infarction significantly decreased necrotic muscle fibers in heart tissue at day 7.
In plain wordsIn rats with a heart attack model, humanin given beforehand reduced dead heart muscle fibers at day 7 compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseNecrotic muscle fibers in heart tissue at day 7
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI group without humanin
Outcome group
Heart function · Cardiovascular
Studied dose
2 mg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
erythrocyte extravasation, congested veins and necrotic muscle fibers at the 7th day of MI in rats given HN before MI.
2 context sentences(design, population, supporting numbers)
Summary · context
The rats were divided into 7 groups each with 6 rats (group I (control), group II (HN 48th hour), group III (HN 7th day), group IV (MI 48th hour), group V (MI 7th day), group VI (MI + HN 48th hour), group VII (MI + HN 7th day).
Summary · context
2 mg/kg HN was given intraperitoneally (ip) to rats alone and before MI.
Show in the study text Report a problemFND-3757626710

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Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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