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Study · Ex vivo tissue · bovine · 2026

9 results

Mitochondrial derived peptide humanin improved semen quality, semen freezability, antioxidant status and in-vitro fertility in crossbred bull.

Gemeda AE, Singh M, Katiyar R, Kumar A, Pande M, Patra MK, Karikalan M, Srivastava N, Chandra V, Singh SK, Ghosh SK

Scientific reports3 Feb 2026Cited by 1 (Europe PMC)

PMID41634054 (opens in a new tab)PMCIDPMC12886930 (opens in a new tab)DOI10.1038/s41598-025-30931-4 (opens in a new tab)PepBankPUB-41634054
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    Before freezing, humanin had no significant effect on the percentage of individual progressive motility in any group of bull semen.

    In plain wordsIn bull semen, humanin did not significantly change sperm motility before freezing at any dose.

    Peptide
    Humanin
    Outcome
    No significant changeProgressive motility before freezing
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    2.5, 5 and 7.5 µM · added to semen extenderAs given in this study (bovine).

    Source sentences

    Summary
    At the pre-freeze stage, there was no significant effect on the percentage of individual progressive motility in any group.
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…
  2. 02LabEx vivo tissueEfficacy outcome

    Supplementation with 5 µM humanin resulted in an 87.5% ejaculate recovery rate after cryopreservation of bull semen.

    In plain wordsIn bull semen, 5 µM humanin gave an 87.5% ejaculate recovery rate after freezing.

    Peptide
    Humanin
    Outcome
    IncreaseEjaculate recovery rate after cryopreservation
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (bovine).

    Measurements

    Ejaculate recovery rate
    87.5%
    5 µM humanin

    Source sentences

    Summary
    Supplementation with 5 µM humanin resulted in an 87.5% ejaculate recovery rate after cryopreservation.
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…
  3. 03LabEx vivo tissueEfficacy outcome

    After thawing, sperm quality parameters were significantly improved in bull semen with 5 µM humanin.

    In plain wordsIn bull semen, 5 µM humanin improved sperm quality measures after thawing.

    Peptide
    Humanin
    Outcome
    IncreasePost-thaw sperm quality parameters
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (bovine).

    Source sentences

    Summary
    At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation).
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…
  4. 04LabEx vivo tissueEfficacy outcome

    After thawing, sperm kinematics were significantly improved in bull semen with 5 µM humanin.

    In plain wordsIn bull semen, 5 µM humanin improved sperm movement measures after thawing.

    Peptide
    Humanin
    Outcome
    IncreasePost-thaw sperm kinematics
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (bovine).

    Source sentences

    Summary
    At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation).
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…
  5. 05LabEx vivo tissueEfficacy outcome

    After thawing, sperm plasma membrane integrity was significantly improved in bull semen with 5 µM humanin.

    In plain wordsIn bull semen, 5 µM humanin improved sperm membrane integrity after thawing.

    Peptide
    Humanin
    Outcome
    IncreasePost-thaw sperm plasma membrane integrity
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (bovine).

    Source sentences

    Summary
    At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation).
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…
  6. 06LabEx vivo tissueEfficacy outcome

    After thawing, mitochondrial membrane potential was significantly improved in bull semen with 5 µM humanin.

    In plain wordsIn bull semen, 5 µM humanin improved sperm mitochondrial membrane potential after thawing.

    Peptide
    Humanin
    Outcome
    IncreasePost-thaw mitochondrial membrane potential
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (bovine).

    Source sentences

    Summary
    At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation).
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…
  7. 07LabEx vivo tissueEfficacy outcome

    After thawing, sperm cryo-capacitation status was significantly improved in bull semen with 5 µM humanin.

    In plain wordsIn bull semen, 5 µM humanin improved sperm cryo-capacitation status after thawing.

    Peptide
    Humanin
    Outcome
    Not applicablePost-thaw sperm cryo-capacitation status
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (bovine).

    Source sentences

    Summary
    At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation).
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…
  8. 08LabEx vivo tissueEfficacy outcome

    After thawing, antioxidant status was significantly improved in bull semen with 5 µM humanin.

    In plain wordsIn bull semen, 5 µM humanin improved antioxidant status after thawing.

    Peptide
    Humanin
    Outcome
    IncreasePost-thaw antioxidant status
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (bovine).

    Source sentences

    Summary
    At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation).
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…
  9. 09LabEx vivo tissueEfficacy outcome

    After thawing, in-vitro fertility was significantly improved in bull semen with 5 µM humanin.

    In plain wordsIn bull semen, 5 µM humanin improved in-vitro fertility results after thawing.

    Peptide
    Humanin
    Outcome
    IncreasePost-thaw in-vitro fertility
    Study

    bovinen = 24

    Crossbred bull semen ejaculates in extender
    Comparator
    Control without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (bovine).

    Source sentences

    Summary
    At the post-thaw stage, sperm quality parameters, sperm kinematics, sperm plasma membrane integrity, mitochondrial membrane potential, sperm cryo-capacitation status, antioxidant status and in-vitro fertility were significantly improved in Group III (5 µM humanin supplementation).
    2 context sentences
    Summary · context
    For this objective a total of 24 ejaculates, 8 from each of the three Vrindavani crossbred bulls, were selected based on sperm progressive motility (≥ 70%) and concentration (≥ 500 million).
    Summary · context
    The selected fresh semen samples were divided into four aliquots and diluted with Tris-Fructose-Egg-Yolk-Glycerol (TFEGY) extender supplemented with various concentrations of humanin peptide; control (Group-1) without supplementation; Group II with 2.5 µM; Group III supplemented with 5 µM and Group…

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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