A novel beneficial role of humanin on intestinal apoptosis and dysmotility in a rat model of ischemia reperfusion injury.

Abozaid ER, Abdel-Kareem RH, Habib MA

Pflugers Archiv : European journal of physiology5 Apr 2023Cited by 11 (Europe PMC)

PMID37020079PMCIDPMC10105677DOI10.1007/s00424-023-02804-0PepBankPUB-37020079

Summary

A prevalent clinical problem including sepsis, shock, necrotizing enterocolitis, and mesenteric thrombosis is intestinal ischemia/reperfusion (I/R) injury. Humanin (HN), a recently identified mitochondrial polypeptide, exhibits antioxidative and antiapoptotic properties. This work aimed to study the role of HN in a model of experimental intestinal I/R injury and its effect on associated dysmotility. A total of 36 male adult albino rats were allocated into 3 equal groups. Sham group: merely a laparotomy was done. I/R group: for 1 h, clamping of the superior mesenteric artery was done, and then reperfusion was allowed for 2 h later. HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN. Small intestinal motility was evaluated, and jejunal samples were got for biochemical and histological analysis. I/R group showed elevation of intestinal NO, MDA, TNF- α, and IL-6 and decline of GPx and SOD levels. Furthermore, histologically, there were destructed jejunal villi especially their tips and increased tissue expression of caspase-3 and i-NOS, in addition to reduced small intestinal motility. Compared to I/R group, HN-I/R group exhibited decrease intestinal levels of NO, MDA, TNF- α, and IL-6 and increase GPx and SOD. Moreover, there was noticeable improvement of the histopathologic features and decreased caspase-3 and iNOS immunoreactivity, beside enhanced small intestinal motility. HN alleviates inflammation, apoptosis, and intestinal dysmotility encouraged by I/R. Additionally, I/R-induced apoptosis and motility alterations depend partly on the production of nitric oxide.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In rats with intestinal ischemia/reperfusion injury, humanin lowered intestinal nitric oxide compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin lowered intestinal nitric oxide compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseIntestinal NO level
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Inflammation markers · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Compared to I/R group, HN-I/R group exhibited decrease intestinal levels of NO, MDA, TNF- α, and IL-6 and increase GPx and SOD.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007901
02AnimalAnimal study (in vivo)Efficacy outcome
In rats with intestinal ischemia/reperfusion injury, humanin lowered intestinal MDA compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin lowered an oxidative stress marker compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseIntestinal MDA level
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Oxidative stress · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Compared to I/R group, HN-I/R group exhibited decrease intestinal levels of NO, MDA, TNF- α, and IL-6 and increase GPx and SOD.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007902
03AnimalAnimal study (in vivo)Efficacy outcome
In rats with intestinal ischemia/reperfusion injury, humanin lowered intestinal TNF-α compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin lowered an inflammation marker compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseIntestinal TNF-α level
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Inflammation markers · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Compared to I/R group, HN-I/R group exhibited decrease intestinal levels of NO, MDA, TNF- α, and IL-6 and increase GPx and SOD.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007903
04AnimalAnimal study (in vivo)Efficacy outcome
In rats with intestinal ischemia/reperfusion injury, humanin lowered intestinal IL-6 compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin lowered an inflammation marker compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseIntestinal IL-6 level
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Inflammation markers · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Compared to I/R group, HN-I/R group exhibited decrease intestinal levels of NO, MDA, TNF- α, and IL-6 and increase GPx and SOD.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007904
05AnimalAnimal study (in vivo)Efficacy outcome
In rats with intestinal ischemia/reperfusion injury, humanin increased intestinal GPx compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin raised an antioxidant enzyme compared with untreated rats.
Peptide
Humanin
Outcome
IncreaseIntestinal GPx level
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Oxidative stress · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Compared to I/R group, HN-I/R group exhibited decrease intestinal levels of NO, MDA, TNF- α, and IL-6 and increase GPx and SOD.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007905
06AnimalAnimal study (in vivo)Efficacy outcome
In rats with intestinal ischemia/reperfusion injury, humanin increased intestinal SOD compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin raised an antioxidant enzyme compared with untreated rats.
Peptide
Humanin
Outcome
IncreaseIntestinal SOD level
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Oxidative stress · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Compared to I/R group, HN-I/R group exhibited decrease intestinal levels of NO, MDA, TNF- α, and IL-6 and increase GPx and SOD.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007906
07AnimalAnimal study (in vivo)Efficacy outcome
In rats with intestinal ischemia/reperfusion injury, humanin improved jejunal histopathologic features compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin improved the look of intestinal tissue compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseJejunal histopathologic damage
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Gastrointestinal protection · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Moreover, there was noticeable improvement of the histopathologic features and decreased caspase-3 and iNOS immunoreactivity, beside enhanced small intestinal motility.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007907
08AnimalAnimal study (in vivo)Mechanism
In rats with intestinal ischemia/reperfusion injury, humanin decreased caspase-3 immunoreactivity compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin lowered a cell death marker compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseJejunal caspase-3 immunoreactivity
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Gastrointestinal protection · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Moreover, there was noticeable improvement of the histopathologic features and decreased caspase-3 and iNOS immunoreactivity, beside enhanced small intestinal motility.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007908
09AnimalAnimal study (in vivo)Mechanism
In rats with intestinal ischemia/reperfusion injury, humanin decreased iNOS immunoreactivity compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin lowered an inflammation-related enzyme compared with untreated rats.
Peptide
Humanin
Outcome
DecreaseJejunal iNOS immunoreactivity
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Inflammation markers · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Moreover, there was noticeable improvement of the histopathologic features and decreased caspase-3 and iNOS immunoreactivity, beside enhanced small intestinal motility.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007909
10AnimalAnimal study (in vivo)Efficacy outcome
In rats with intestinal ischemia/reperfusion injury, humanin enhanced small intestinal motility compared with untreated injured rats.
In plain wordsIn rats with intestinal injury, humanin improved gut movement compared with untreated rats.
Peptide
Humanin
Outcome
IncreaseSmall intestinal motility
Study
ratn: not reported in source
Intestinal ischemia/reperfusion injury
Comparator
I/R group
Outcome group
Gastrointestinal protection · Gastrointestinal
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Moreover, there was noticeable improvement of the histopathologic features and decreased caspase-3 and iNOS immunoreactivity, beside enhanced small intestinal motility.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 36 male adult albino rats were allocated into 3 equal groups.
Summary · context
HN-I/R group: rats underwent ischemia and reperfusion, and 30 min before the reperfusion, they received an intraperitoneal injection of 252 μg/kg of HN.
Show in the study text Report a problemFND-3702007910

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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