Cryoprotectant with a mitochondrial derived peptide, humanin, improves post-thaw quality of buffalo spermatozoa.

Katiyar R , Ghosh SK , Kumar A , Pande M , Gemeda AE , Rautela R , Bhure SK , Dhara SK , Mathesh K , Srivastava N , Patra MK

Cryo letters1 Jan 2022Cited by 3 (Europe PMC)

PMID35315868DOI10.54680/fr22110110212PepBankPUB-35315868

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo semen, humanin at 2, 5 and 10 µM gave significantly higher post-thaw progressive motility than control.
In plain wordsIn lab tests on buffalo semen, humanin at each dose gave higher motility after thawing than control.
Peptide
Humanin
Outcome
IncreasePost-thaw sperm motility
Study
buffalon = 18
buffalo bull semen frozen in egg yolk-tris-glycerol extender
Comparator
Control extender without humanin
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
2, 5 and 10 µM · added to semen extenderAs given in this study (buffalo).
Source sentences
Summary
Humanin supplementation resulted in significantly higher (p < 0.05) post-thaw motility in all treatment groups and, higher (p < 0.05) viability in Groups III and IV in comparison to the control at the post-thaw stage.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 18 ejaculates from three Murrah buffalo bulls (n=6 each) were collected.
Summary · context
The first aliquot was diluted with standard EYTG dilutor (Group I, control), whereas the other three aliquots were diluted with EYTG supplemented with 2 µM (Group II), 5 µM (Group III) and 10 µM humanin (Group IV), respectively.
Report a problemFND-3531586801
02LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo semen, humanin at 5 and 10 µM gave significantly higher post-thaw viability than control.
In plain wordsIn lab tests on buffalo semen, humanin at 5 and 10 µM kept more sperm alive after thawing than control.
Peptide
Humanin
Outcome
IncreasePost-thaw sperm viability
Study
buffalon = 18
buffalo bull semen frozen in egg yolk-tris-glycerol extender
Comparator
Control extender without humanin
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
5 and 10 µM · added to semen extenderAs given in this study (buffalo).
Source sentences
Summary
Humanin supplementation resulted in significantly higher (p < 0.05) post-thaw motility in all treatment groups and, higher (p < 0.05) viability in Groups III and IV in comparison to the control at the post-thaw stage.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 18 ejaculates from three Murrah buffalo bulls (n=6 each) were collected.
Summary · context
The first aliquot was diluted with standard EYTG dilutor (Group I, control), whereas the other three aliquots were diluted with EYTG supplemented with 2 µM (Group II), 5 µM (Group III) and 10 µM humanin (Group IV), respectively.
Report a problemFND-3531586802
03LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo semen, humanin at 5 and 10 µM gave more spermatozoa with intact acrosome than control or 2 µM.
In plain wordsIn lab tests on buffalo semen, humanin at 5 and 10 µM kept more sperm acrosomes intact than lower doses.
Peptide
Humanin
Outcome
IncreaseIntact acrosome
Study
buffalon = 18
buffalo bull semen frozen in egg yolk-tris-glycerol extender
Comparator
Control and 2 µM humanin
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
5 and 10 µM · added to semen extenderAs given in this study (buffalo).
Source sentences
Summary
Spermatozoa with intact acrosome and plasma membrane were higher (p < 0.05) in Groups III and IV as compared to Groups I and II.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 18 ejaculates from three Murrah buffalo bulls (n=6 each) were collected.
Summary · context
The first aliquot was diluted with standard EYTG dilutor (Group I, control), whereas the other three aliquots were diluted with EYTG supplemented with 2 µM (Group II), 5 µM (Group III) and 10 µM humanin (Group IV), respectively.
Report a problemFND-3531586803
04LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo semen, humanin at 5 and 10 µM gave more spermatozoa with intact plasma membrane than control or 2 µM.
In plain wordsIn lab tests on buffalo semen, humanin at 5 and 10 µM kept more sperm membranes intact than lower doses.
Peptide
Humanin
Outcome
IncreaseIntact plasma membrane
Study
buffalon = 18
buffalo bull semen frozen in egg yolk-tris-glycerol extender
Comparator
Control and 2 µM humanin
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
5 and 10 µM · added to semen extenderAs given in this study (buffalo).
Source sentences
Summary
Spermatozoa with intact acrosome and plasma membrane were higher (p < 0.05) in Groups III and IV as compared to Groups I and II.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 18 ejaculates from three Murrah buffalo bulls (n=6 each) were collected.
Summary · context
The first aliquot was diluted with standard EYTG dilutor (Group I, control), whereas the other three aliquots were diluted with EYTG supplemented with 2 µM (Group II), 5 µM (Group III) and 10 µM humanin (Group IV), respectively.
Report a problemFND-3531586804
05LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo semen, humanin at 2, 5 and 10 µM gave lower lipid peroxidation than control.
In plain wordsIn lab tests on buffalo semen, humanin at each dose lowered a marker of oxidative damage after thawing.
Peptide
Humanin
Outcome
DecreasePost-thaw lipid peroxidation (LPO)
Study
buffalon = 18
buffalo bull semen frozen in egg yolk-tris-glycerol extender
Comparator
Control extender without humanin
Outcome group
Oxidative stress · Reproductive and sexual health
Studied dose
2, 5 and 10 µM · added to semen extenderAs given in this study (buffalo).
Source sentences
Summary
The LPO levels at the post-thaw stage were found to be lower (p < 0.05) in all treatment groups versus the control group
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 18 ejaculates from three Murrah buffalo bulls (n=6 each) were collected.
Summary · context
The first aliquot was diluted with standard EYTG dilutor (Group I, control), whereas the other three aliquots were diluted with EYTG supplemented with 2 µM (Group II), 5 µM (Group III) and 10 µM humanin (Group IV), respectively.
Report a problemFND-3531586805
06LabEx vivo tissueEfficacy outcome
In frozen-thawed buffalo semen, humanin at 5 and 10 µM gave higher total antioxidant capacity than control or 2 µM.
In plain wordsIn lab tests on buffalo semen, humanin at 5 and 10 µM raised antioxidant capacity more than control or the lower dose.
Peptide
Humanin
Outcome
IncreasePost-thaw total antioxidant capacity (TAC)
Study
buffalon = 18
buffalo bull semen frozen in egg yolk-tris-glycerol extender
Comparator
Control and 2 µM humanin
Outcome group
Oxidative stress · Reproductive and sexual health
Studied dose
5 and 10 µM · added to semen extenderAs given in this study (buffalo).
Source sentences
Summary
higher (p≤0.05) TAC values were recorded in Groups III and IV in comparison to the control and Group II.
2 context sentences(design, population, supporting numbers)
Summary · context
A total of 18 ejaculates from three Murrah buffalo bulls (n=6 each) were collected.
Summary · context
The first aliquot was diluted with standard EYTG dilutor (Group I, control), whereas the other three aliquots were diluted with EYTG supplemented with 2 µM (Group II), 5 µM (Group III) and 10 µM humanin (Group IV), respectively.
Report a problemFND-3531586806

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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