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Study · Animal study (in vivo) · mouse · 2023

13 results

S14G-Humanin ameliorates ovalbumin-induced airway inflammation in asthma mediated by inhibition of toll-like receptor 4 (TLR4) expression and the nuclear factor κ-B (NF-κB)/early growth response protein-1 (Egr-1) pathway.

Su B, Li R, Song F, Liu M, Sun X

Aging14 Jul 2023Cited by 5 (Europe PMC)

PMID37451839 (opens in a new tab)PMCIDPMC10415557 (opens in a new tab)DOI10.18632/aging.204874 (opens in a new tab)PepBankPUB-37451839
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Summary

Asthma is a chronic inflammatory disease with a high morbidity rate in children and significantly impacts their healthy growth. It is reported that Th2 cell-mediated airway inflammation and activated oxidative stress are involved in the pathogenesis of asthma. S14G-humanin (HNG) is a derivative of Humanin with higher activity. The present study proposes to explore the potential treating property of HNG on asthma. An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days. Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG. Furthermore, the increased malondialdehyde (MDA) level and declined superoxide dismutase (SOD) activity in OVA-challenged mice were abolished by 2.5 mg/kg and 5 mg/kg HNG. Lastly, the upregulated TLR4, p-NF-κB p65, and early growth response 1 (Egr-1) in lung tissues of OVA-challenged mice were pronouncedly downregulated by 2.5 mg/kg and 5 mg/kg HNG. Collectively, our data suggested that HNG ameliorated airway inflammation in asthma partially due to NF-κB and Egr-1-mediated responses.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study13

13 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin alleviated the increased lung weight index.

    In plain wordsIn mice with asthma, a humanin analog reduced lung weight increase compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseLung weight index
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin alleviated the elevated number of monocytes.

    In plain wordsIn mice with asthma, a humanin analog lowered monocyte counts compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseMonocyte count
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin alleviated the elevated number of eosinophils.

    In plain wordsIn mice with asthma, a humanin analog lowered eosinophil counts compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseEosinophil count
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin alleviated the elevated number of neutrophils.

    In plain wordsIn mice with asthma, a humanin analog lowered neutrophil counts compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseNeutrophil count
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin alleviated the increased production of IL-4.

    In plain wordsIn mice with asthma, a humanin analog lowered an allergy-related signal compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseIL-4 production
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  6. 06AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin alleviated the increased production of IL-5.

    In plain wordsIn mice with asthma, a humanin analog lowered an allergy-related signal compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseIL-5 production
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  7. 07AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin alleviated the increased production of IL-13.

    In plain wordsIn mice with asthma, a humanin analog lowered an allergy-related signal compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseIL-13 production
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  8. 08AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin alleviated severe histological pathology.

    In plain wordsIn mice with asthma, a humanin analog improved lung tissue damage compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseLung histological pathology
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dramatically increased lung weight index, elevated number of monocytes, eosinophils, and neutrophils, promoted production of Th2 cytokines including interleukin-4 (IL-4), interleukin-5 (IL-5), and interleukin-13 (IL-13), and severe histological pathology were observed in OVA-challenged mice, all of which were extremely alleviated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  9. 09AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin abolished the increased MDA level.

    In plain wordsIn mice with asthma, a humanin analog lowered an oxidative stress marker compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseMalondialdehyde (MDA) level
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Oxidative stress · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Furthermore, the increased malondialdehyde (MDA) level and declined superoxide dismutase (SOD) activity in OVA-challenged mice were abolished by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  10. 10AnimalAnimal study (in vivo)Efficacy outcome

    In mice with ovalbumin-induced asthma, S14G-humanin abolished the decline in SOD activity.

    In plain wordsIn mice with asthma, a humanin analog restored an antioxidant enzyme compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    IncreaseSuperoxide dismutase (SOD) activity
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Oxidative stress · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Furthermore, the increased malondialdehyde (MDA) level and declined superoxide dismutase (SOD) activity in OVA-challenged mice were abolished by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  11. 11AnimalAnimal study (in vivo)Mechanism

    In mice with ovalbumin-induced asthma, S14G-humanin downregulated the upregulated TLR4 in lung tissue.

    In plain wordsIn mice with asthma, a humanin analog lowered an inflammation receptor in the lungs compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseLung TLR4 expression
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Lastly, the upregulated TLR4, p-NF-κB p65, and early growth response 1 (Egr-1) in lung tissues of OVA-challenged mice were pronouncedly downregulated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  12. 12AnimalAnimal study (in vivo)Mechanism

    In mice with ovalbumin-induced asthma, S14G-humanin downregulated the upregulated p-NF-κB p65 in lung tissue.

    In plain wordsIn mice with asthma, a humanin analog lowered an inflammation pathway protein in the lungs compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseLung p-NF-κB p65 expression
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Lastly, the upregulated TLR4, p-NF-κB p65, and early growth response 1 (Egr-1) in lung tissues of OVA-challenged mice were pronouncedly downregulated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.
  13. 13AnimalAnimal study (in vivo)Mechanism

    In mice with ovalbumin-induced asthma, S14G-humanin downregulated the upregulated Egr-1 in lung tissue.

    In plain wordsIn mice with asthma, a humanin analog lowered an inflammation pathway protein in the lungs compared with untreated asthmatic mice.

    Peptide
    Humanin
    Outcome
    DecreaseLung Egr-1 expression
    Study

    mousen: not reported in source

    Ovalbumin-induced asthma
    Comparator
    OVA-challenged mice
    Outcome group
    Inflammation markers · Respiratory
    Studied dose
    2.5 mg/kg and 5 mg/kg · 16 daysAs given in this study (mouse).

    Source sentences

    Summary
    Lastly, the upregulated TLR4, p-NF-κB p65, and early growth response 1 (Egr-1) in lung tissues of OVA-challenged mice were pronouncedly downregulated by 2.5 mg/kg and 5 mg/kg HNG.
    1 context sentence
    Summary · context
    An asthma model was constructed in mice using ovalbumin (OVA), the mice were treated with 2.5 mg/kg and 5 mg/kg HNG for 16 days.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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