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Study · Animal study (in vivo) · mouse · 2020

11 results

Humanin analogue, HNG, inhibits platelet activation and thrombus formation by stabilizing platelet microtubules.

Ren L, Li Q, You T, Zhao X, Xu X, Tang C, Zhu L

Journal of cellular and molecular medicine16 Mar 2020Cited by 16 (Europe PMC)

PMID32174022 (opens in a new tab)PMCIDPMC7176859 (opens in a new tab)DOI10.1111/jcmm.15151 (opens in a new tab)PepBankPUB-32174022
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Summary

HNG, a highly potent mutant of the anti-Alzheimer peptide-humanin, has been shown to protect against ischaemia-reperfusion (I/R) injury. However, the underlying mechanism related to platelet activation remains unknown. We proposed that HNG has an effect on platelet function and thrombus formation. In this study, platelet aggregation, granule secretion, clot retraction, integrin activation and adhesion under flow conditions were evaluated. In mice receiving HNG or saline, cremaster arterial thrombus formation induced by laser injury, tail bleeding time and blood loss were recorded. Platelet microtubule depolymerization was evaluated using immunofluorescence staining. Results showed that HNG inhibited platelet aggregation, P-selectin expression, ATP release, and αIIb β3 activation and adhesion under flow conditions. Mice receiving HNG had attenuated cremaster arterial thrombus formation, although the bleeding time was not prolonged. Moreover, HNG significantly inhibited microtubule depolymerization, enhanced tubulin acetylation in platelets stimulated by fibrinogen or microtubule depolymerization reagent, nocodazole, and inhibited AKT and ERK phosphorylation downstream of HDAC6 by collagen stimulation. Therefore, our results identified a novel role of HNG in platelet function and thrombus formation potentially through stabilizing platelet microtubules via tubulin acetylation. These findings suggest a potential benefit of HNG in the management of cardiovascular diseases.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study11

11 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    Mice receiving HNG had attenuated laser-induced cremaster arterial thrombus formation compared with saline.

    In plain wordsIn mice, HNG reduced blood clot formation in an injured artery compared with saline.

    Peptide
    Humanin
    Outcome
    DecreaseCremaster arterial thrombus formation
    Study

    mousen: not reported in source

    laser-injured cremaster arterioles
    Comparator
    Saline
    Outcome group
    Cardiovascular events · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Mice receiving HNG had attenuated cremaster arterial thrombus formation, although the bleeding time was not prolonged.
    1 context sentence
    Summary · context
    In mice receiving HNG or saline, cremaster arterial thrombus formation induced by laser injury, tail bleeding time and blood loss were recorded.
  2. 02AnimalAnimal study (in vivo)Safety outcome

    Bleeding time was not prolonged in mice receiving HNG compared with saline.

    In plain wordsIn mice, HNG did not make bleeding time longer than saline.

    Peptide
    Humanin
    Outcome
    No significant changeTail bleeding time
    Study

    mousen: not reported in source

    tail bleeding test
    Comparator
    Saline
    Outcome group
    Adverse events · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    although the bleeding time was not prolonged.
    1 context sentence
    Summary · context
    In mice receiving HNG or saline, cremaster arterial thrombus formation induced by laser injury, tail bleeding time and blood loss were recorded.
  3. 03LabIn vitro (cells)Efficacy outcome

    The humanin analogue HNG inhibited platelet aggregation in platelet function assays.

    In plain wordsIn lab tests, the humanin analogue HNG reduced platelet clumping.

    Peptide
    Humanin
    Outcome
    DecreasePlatelet aggregation
    Study

    n: not reported in source

    platelets
    Outcome group
    Cardiovascular events · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results showed that HNG inhibited platelet aggregation, P-selectin expression, ATP release, and αIIb β3 activation and adhesion under flow conditions.
    1 context sentence
    Summary · context
    In this study, platelet aggregation, granule secretion, clot retraction, integrin activation and adhesion under flow conditions were evaluated.
  4. 04LabIn vitro (cells)Efficacy outcome

    HNG inhibited P-selectin expression on platelets.

    In plain wordsIn lab tests, HNG lowered a platelet activation marker called P-selectin.

    Peptide
    Humanin
    Outcome
    DecreaseP-selectin expression
    Study

    n: not reported in source

    platelets
    Outcome group
    Cardiovascular events · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results showed that HNG inhibited platelet aggregation, P-selectin expression, ATP release, and αIIb β3 activation and adhesion under flow conditions.
    1 context sentence
    Summary · context
    In this study, platelet aggregation, granule secretion, clot retraction, integrin activation and adhesion under flow conditions were evaluated.
  5. 05LabIn vitro (cells)Efficacy outcome

    HNG inhibited ATP release from platelets.

    In plain wordsIn lab tests, HNG reduced ATP release from platelets.

    Peptide
    Humanin
    Outcome
    DecreaseATP release
    Study

    n: not reported in source

    platelets
    Outcome group
    Cardiovascular events · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results showed that HNG inhibited platelet aggregation, P-selectin expression, ATP release, and αIIb β3 activation and adhesion under flow conditions.
    1 context sentence
    Summary · context
    In this study, platelet aggregation, granule secretion, clot retraction, integrin activation and adhesion under flow conditions were evaluated.
  6. 06LabIn vitro (cells)Efficacy outcome

    HNG inhibited integrin activation in platelets.

    In plain wordsIn lab tests, HNG reduced activation of a platelet integrin.

    Peptide
    Humanin
    Outcome
    DecreaseIntegrin activation
    Study

    n: not reported in source

    platelets
    Outcome group
    Cardiovascular events · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results showed that HNG inhibited platelet aggregation, P-selectin expression, ATP release, and αIIb β3 activation and adhesion under flow conditions.
    1 context sentence
    Summary · context
    In this study, platelet aggregation, granule secretion, clot retraction, integrin activation and adhesion under flow conditions were evaluated.
  7. 07LabIn vitro (cells)Efficacy outcome

    HNG inhibited platelet adhesion under flow conditions.

    In plain wordsIn lab tests, HNG reduced platelet sticking under flow.

    Peptide
    Humanin
    Outcome
    DecreasePlatelet adhesion under flow
    Study

    n: not reported in source

    platelets
    Outcome group
    Cardiovascular events · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results showed that HNG inhibited platelet aggregation, P-selectin expression, ATP release, and αIIb β3 activation and adhesion under flow conditions.
    1 context sentence
    Summary · context
    In this study, platelet aggregation, granule secretion, clot retraction, integrin activation and adhesion under flow conditions were evaluated.
  8. 08LabIn vitro (cells)Mechanism

    HNG inhibited microtubule depolymerization in platelets stimulated by fibrinogen or nocodazole.

    In plain wordsIn lab tests, HNG kept platelet microtubules from breaking down.

    Peptide
    Humanin
    Outcome
    DecreasePlatelet microtubule depolymerization
    Study

    n: not reported in source

    platelets
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, HNG significantly inhibited microtubule depolymerization, enhanced tubulin acetylation in platelets stimulated by fibrinogen or microtubule depolymerization reagent, nocodazole, and inhibited AKT and ERK phosphorylation downstream of HDAC6 by collagen stimulation.
    1 context sentence
    Summary · context
    Platelet microtubule depolymerization was evaluated using immunofluorescence staining.
  9. 09LabIn vitro (cells)Mechanism

    HNG enhanced tubulin acetylation in platelets stimulated by fibrinogen or nocodazole.

    In plain wordsIn lab tests, HNG increased tubulin acetylation in platelets.

    Peptide
    Humanin
    Outcome
    IncreaseTubulin acetylation
    Study

    n: not reported in source

    platelets
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, HNG significantly inhibited microtubule depolymerization, enhanced tubulin acetylation in platelets stimulated by fibrinogen or microtubule depolymerization reagent, nocodazole, and inhibited AKT and ERK phosphorylation downstream of HDAC6 by collagen stimulation.
    1 context sentence
    Summary · context
    Platelet microtubule depolymerization was evaluated using immunofluorescence staining.
  10. 10LabIn vitro (cells)Mechanism

    HNG inhibited AKT phosphorylation downstream of HDAC6 after collagen stimulation of platelets.

    In plain wordsIn lab tests, HNG reduced AKT signalling in collagen-stimulated platelets.

    Peptide
    Humanin
    Outcome
    DecreaseAKT phosphorylation after collagen stimulation
    Study

    n: not reported in source

    platelets
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, HNG significantly inhibited microtubule depolymerization, enhanced tubulin acetylation in platelets stimulated by fibrinogen or microtubule depolymerization reagent, nocodazole, and inhibited AKT and ERK phosphorylation downstream of HDAC6 by collagen stimulation.
    1 context sentence
    Summary · context
    Platelet microtubule depolymerization was evaluated using immunofluorescence staining.
  11. 11LabIn vitro (cells)Mechanism

    HNG inhibited ERK phosphorylation downstream of HDAC6 after collagen stimulation of platelets.

    In plain wordsIn lab tests, HNG reduced ERK signalling in collagen-stimulated platelets.

    Peptide
    Humanin
    Outcome
    DecreaseERK phosphorylation after collagen stimulation
    Study

    n: not reported in source

    platelets
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, HNG significantly inhibited microtubule depolymerization, enhanced tubulin acetylation in platelets stimulated by fibrinogen or microtubule depolymerization reagent, nocodazole, and inhibited AKT and ERK phosphorylation downstream of HDAC6 by collagen stimulation.
    1 context sentence
    Summary · context
    Platelet microtubule depolymerization was evaluated using immunofluorescence staining.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

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Terms as the source lists them.

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