Effect of humanine on the Notch signaling pathway in myocardial infarction.

Onat E, Önalan E, Önalan E, Özdem B, Kavak Balgetir M, Kuloğlu T

Turkish journal of medical sciences25 Oct 2023Cited by 3 (Europe PMC)

PMID38813496PMCIDPMC10760541DOI10.55730/1300-0144.5734PepBankPUB-38813496

Summary

Background/aimBy applying humanin (HN) before myocardial infarction (MI), its protection in myocardial injury and the possible roles of its cellular mechanism in the Notch pathway were investigated.Materials and methodsThe study was carried out at Fırat University Experimental Research Center (12/24/2018-12/23/2019). Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7). To create MI, 200 mg/kg of isoproterenol (ISO) was administered to the rats subcutaneously. Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI. Molecular parameters Notch1, Notch2, Hes1, Hes2, Jagged1, Jagged2, DLL1, and DLL4 were examined using polymerase chain reaction in the heart tissue, Notch1, Hes1, and DLL4 were examined using western blot, while heart tissue was taken for histochemical examinations.ResultsThe mRNA expression levels of the Notch signaling members (Notch1, Notch2, Hes1, Hes2, Jagged1, Jagged2, DLL1, and DLL4) tended to decrease after MI. The Notch signaling members increased more significantly, especially toward day 7 after HN application before MI. In the western blot anylyses, the Notch1, Hes1, and DLL4 protein levels increased significantly toward day 7 in the groups given HN before MI. Moreover, the serum AST, LDH, CK-MB, and troponin I levels tended to decrease with the application of HN before MI and there was a significant decrease in edema, hemorrhage, and mononuclear cells in the heart tissue at 24 h post-MI and fibrosis on day 7 post-MI.ConclusionHN administration before MI has a cardioprotective effect on rats via the Notch signaling pathway.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In rats with myocardial infarction, humanin given before MI increased mRNA expression of Notch signaling members, especially toward day 7.
In plain wordsIn rats with a heart attack, humanin given beforehand raised Notch pathway gene activity, mostly by day 7.
Peptide
Humanin
Outcome
IncreaseNotch signaling member mRNA expression
Study
ratn = 14
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
The Notch signaling members increased more significantly, especially toward day 7 after HN application before MI.
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349601
02AnimalAnimal study (in vivo)Mechanism
In rats with myocardial infarction, humanin given before MI increased Notch1, Hes1 and DLL4 protein levels toward day 7.
In plain wordsIn rats with a heart attack, humanin given beforehand raised three Notch pathway proteins by day 7.
Peptide
Humanin
Outcome
IncreaseNotch1, Hes1 and DLL4 protein levels
Study
ratn = 14
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
In the western blot anylyses, the Notch1, Hes1, and DLL4 protein levels increased significantly toward day 7 in the groups given HN before MI.
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349602
03AnimalAnimal study (in vivo)Efficacy outcome
In rats with myocardial infarction, humanin given before MI reduced edema in heart tissue at 24 h post-MI.
In plain wordsIn rats with a heart attack, humanin given beforehand reduced swelling in heart tissue at 24 hours.
Peptide
Humanin
Outcome
DecreaseHeart tissue edema at 24 h post-MI
Study
ratn = 14
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Heart function · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
there was a significant decrease in edema, hemorrhage, and mononuclear cells in the heart tissue at 24 h post-MI and fibrosis on day 7 post-MI.
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349607
04AnimalAnimal study (in vivo)Efficacy outcome
In rats with myocardial infarction, humanin given before MI reduced hemorrhage in heart tissue at 24 h post-MI.
In plain wordsIn rats with a heart attack, humanin given beforehand reduced bleeding in heart tissue at 24 hours.
Peptide
Humanin
Outcome
DecreaseHeart tissue hemorrhage at 24 h post-MI
Study
ratn = 14
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Heart function · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
there was a significant decrease in edema, hemorrhage, and mononuclear cells in the heart tissue at 24 h post-MI and fibrosis on day 7 post-MI.
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349608
05AnimalAnimal study (in vivo)Efficacy outcome
In rats with myocardial infarction, humanin given before MI reduced mononuclear cells in heart tissue at 24 h post-MI.
In plain wordsIn rats with a heart attack, humanin given beforehand reduced immune cells in heart tissue at 24 hours.
Peptide
Humanin
Outcome
DecreaseMononuclear cells in heart tissue at 24 h post-MI
Study
ratn = 14
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Inflammation markers · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
there was a significant decrease in edema, hemorrhage, and mononuclear cells in the heart tissue at 24 h post-MI and fibrosis on day 7 post-MI.
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349609
06AnimalAnimal study (in vivo)Efficacy outcome
In rats with myocardial infarction, humanin given before MI reduced fibrosis in heart tissue on day 7 post-MI.
In plain wordsIn rats with a heart attack, humanin given beforehand reduced scarring in heart tissue at 7 days.
Peptide
Humanin
Outcome
DecreaseHeart tissue fibrosis on day 7 post-MI
Study
ratn = 14
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Heart function · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
there was a significant decrease in edema, hemorrhage, and mononuclear cells in the heart tissue at 24 h post-MI and fibrosis on day 7 post-MI.
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349610
07AnimalAnimal study (in vivo)Efficacy outcome
In rats with myocardial infarction, serum AST tended to decrease with humanin given before MI.
In plain wordsIn rats with a heart attack, humanin given beforehand tended to lower the injury marker AST.
Peptide
Humanin
Outcome
DecreaseSerum AST
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Heart function · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Moreover, the serum AST, LDH, CK-MB, and troponin I levels tended to decrease with the application of HN before MI
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349603
08AnimalAnimal study (in vivo)Efficacy outcome
In rats with myocardial infarction, serum LDH tended to decrease with humanin given before MI.
In plain wordsIn rats with a heart attack, humanin given beforehand tended to lower the injury marker LDH.
Peptide
Humanin
Outcome
DecreaseSerum LDH
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Heart function · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Moreover, the serum AST, LDH, CK-MB, and troponin I levels tended to decrease with the application of HN before MI
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349604
09AnimalAnimal study (in vivo)Efficacy outcome
In rats with myocardial infarction, serum CK-MB tended to decrease with humanin given before MI.
In plain wordsIn rats with a heart attack, humanin given beforehand tended to lower the injury marker CK-MB.
Peptide
Humanin
Outcome
DecreaseSerum CK-MB
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Heart function · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Moreover, the serum AST, LDH, CK-MB, and troponin I levels tended to decrease with the application of HN before MI
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349605
10AnimalAnimal study (in vivo)Efficacy outcome
In rats with myocardial infarction, serum troponin I tended to decrease with humanin given before MI.
In plain wordsIn rats with a heart attack, humanin given beforehand tended to lower troponin I.
Peptide
Humanin
Outcome
DecreaseSerum troponin I
Study
ratn: not reported in source
Isoproterenol-induced myocardial infarction
Comparator
MI without HN
Outcome group
Heart function · Cardiovascular
Studied dose
252 μg/kg · intraperitonealAs given in this study (rat).
Source sentences
Summary
Moreover, the serum AST, LDH, CK-MB, and troponin I levels tended to decrease with the application of HN before MI
2 context sentences(design, population, supporting numbers)
Summary · context
Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7).
Summary · context
Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI.
Show in the study text Report a problemFND-3881349606

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

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PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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