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Study · Ex vivo tissue · buffalo · 2022

11 results

An evidence of Humanin-like peptide and Humanin mediated cryosurvival of spermatozoa in buffalo bulls.

Katiyar R, Ghosh SK, Karikalan M, Kumar A, Pande M, Gemeda AI, Rautela R, Dhara SK, Bhure SK, Srivastava N, Patra MK, Chandra V, Devi HL, Singh M

Theriogenology26 Sept 2022Cited by 15 (Europe PMC)

PMID36183493 (opens in a new tab)DOI10.1016/j.theriogenology.2022.09.013 (opens in a new tab)PepBankPUB-36183493
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

8 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In frozen-thawed buffalo semen, humanin at 5 and 10 µM lowered lipid peroxidation.

    In plain wordsIn lab tests on buffalo semen, humanin at 5 and 10 µM lowered a marker of oxidative damage.

    Peptide
    Humanin
    Outcome
    DecreasePost-thaw lipid peroxidation (LPO)
    Study

    buffalon = 24

    buffalo semen frozen in egg yolk-tris-glycerol extender
    Comparator
    Control extender without humanin
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    5 and 10 µM · added to semen extenderAs given in this study (buffalo).

    Source sentences

    Summary
    The LPO levels were lower (p < 0.05) in 5 and 10 μM Humanin supplemented group.
    2 context sentences
    Summary · context
    For supplementation studies, a total of 24 ejaculates (IPM ≥70%) were collected and each ejaculate was then divided into four aliquots.
    Summary · context
    Rest three aliquots were diluted with extender containing 2 (Group II), 5 (Group III) and 10 μM Humanin (Group IV), respectively.
  2. 02LabEx vivo tissueEfficacy outcome

    In frozen-thawed buffalo semen, humanin at 5 µM gave higher mitochondrial membrane potential than other groups.

    In plain wordsIn lab tests on buffalo semen, humanin at 5 µM gave higher sperm mitochondrial function than other groups.

    Peptide
    Humanin
    Outcome
    IncreasePost-thaw mitochondrial membrane potential (MMP)
    Study

    buffalon = 24

    buffalo semen frozen in egg yolk-tris-glycerol extender
    Comparator
    Control and other humanin doses
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (buffalo).

    Source sentences

    Summary
    The MMP and DNA integrity were higher (p < 0.05)
    3 context sentences
    Summary · context
    The MMP and DNA integrity were higher (p < 0.05) in 5 μM group than other groups.
    Summary · context
    For supplementation studies, a total of 24 ejaculates (IPM ≥70%) were collected and each ejaculate was then divided into four aliquots.
    Summary · context
    Rest three aliquots were diluted with extender containing 2 (Group II), 5 (Group III) and 10 μM Humanin (Group IV), respectively.
  3. 03LabEx vivo tissueEfficacy outcome

    In frozen-thawed buffalo semen, humanin at 5 µM gave higher DNA integrity than other groups.

    In plain wordsIn lab tests on buffalo semen, humanin at 5 µM kept sperm DNA more intact than other groups.

    Peptide
    Humanin
    Outcome
    IncreasePost-thaw DNA integrity
    Study

    buffalon = 24

    buffalo semen frozen in egg yolk-tris-glycerol extender
    Comparator
    Control and other humanin doses
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (buffalo).

    Source sentences

    Summary
    The MMP and DNA integrity were higher (p < 0.05) in 5 μM group than other groups.
    2 context sentences
    Summary · context
    For supplementation studies, a total of 24 ejaculates (IPM ≥70%) were collected and each ejaculate was then divided into four aliquots.
    Summary · context
    Rest three aliquots were diluted with extender containing 2 (Group II), 5 (Group III) and 10 μM Humanin (Group IV), respectively.
  4. 04LabEx vivo tissueEfficacy outcome

    In frozen-thawed buffalo semen, humanin at 5 and 10 µM gave a higher F-pattern than control.

    In plain wordsIn lab tests on buffalo semen, humanin at 5 and 10 µM raised the F-pattern, a sperm capacitation measure.

    Peptide
    Humanin
    Outcome
    IncreaseCapacitation F-pattern
    Study

    buffalon = 24

    buffalo semen frozen in egg yolk-tris-glycerol extender
    Comparator
    Control extender without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 and 10 µM · added to semen extenderAs given in this study (buffalo).

    Source sentences

    Summary
    F-pattern was higher (p < 0.05)
    3 context sentences
    Summary · context
    F-pattern was higher (p < 0.05) and B-pattern was lower (p < 0.05) in 5 and 10 μM Humanin supplemented groups.
    Summary · context
    For supplementation studies, a total of 24 ejaculates (IPM ≥70%) were collected and each ejaculate was then divided into four aliquots.
    Summary · context
    Rest three aliquots were diluted with extender containing 2 (Group II), 5 (Group III) and 10 μM Humanin (Group IV), respectively.
  5. 05LabEx vivo tissueEfficacy outcome

    In frozen-thawed buffalo semen, humanin at 5 and 10 µM gave a lower B-pattern than control.

    In plain wordsIn lab tests on buffalo semen, humanin at 5 and 10 µM lowered the B-pattern, a sperm capacitation measure.

    Peptide
    Humanin
    Outcome
    DecreaseCapacitation B-pattern
    Study

    buffalon = 24

    buffalo semen frozen in egg yolk-tris-glycerol extender
    Comparator
    Control extender without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 and 10 µM · added to semen extenderAs given in this study (buffalo).

    Source sentences

    Summary
    F-pattern was higher (p < 0.05) and B-pattern was lower (p < 0.05) in 5 and 10 μM Humanin supplemented groups.
    2 context sentences
    Summary · context
    For supplementation studies, a total of 24 ejaculates (IPM ≥70%) were collected and each ejaculate was then divided into four aliquots.
    Summary · context
    Rest three aliquots were diluted with extender containing 2 (Group II), 5 (Group III) and 10 μM Humanin (Group IV), respectively.
  6. 06LabEx vivo tissueEfficacy outcome

    In frozen-thawed buffalo semen, humanin at 5 µM gave fewer apoptotic spermatozoa.

    In plain wordsIn lab tests on buffalo semen, humanin at 5 µM gave fewer dying sperm.

    Peptide
    Humanin
    Outcome
    DecreaseApoptotic spermatozoa
    Study

    buffalon = 24

    buffalo semen frozen in egg yolk-tris-glycerol extender
    Comparator
    Control extender without humanin
    Outcome group
    Cell viability and death · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (buffalo).

    Source sentences

    Summary
    Lower apoptotic and higher viable spermatozoa (p < 0.05) were observed in 5 μM Humanin group.
    2 context sentences
    Summary · context
    For supplementation studies, a total of 24 ejaculates (IPM ≥70%) were collected and each ejaculate was then divided into four aliquots.
    Summary · context
    Rest three aliquots were diluted with extender containing 2 (Group II), 5 (Group III) and 10 μM Humanin (Group IV), respectively.
  7. 07LabEx vivo tissueEfficacy outcome

    In frozen-thawed buffalo semen, humanin at 5 µM gave more viable spermatozoa.

    In plain wordsIn lab tests on buffalo semen, humanin at 5 µM gave more living sperm.

    Peptide
    Humanin
    Outcome
    IncreaseViable spermatozoa
    Study

    buffalon = 24

    buffalo semen frozen in egg yolk-tris-glycerol extender
    Comparator
    Control extender without humanin
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (buffalo).

    Source sentences

    Summary
    Lower apoptotic and higher viable spermatozoa (p < 0.05) were observed in 5 μM Humanin group.
    2 context sentences
    Summary · context
    For supplementation studies, a total of 24 ejaculates (IPM ≥70%) were collected and each ejaculate was then divided into four aliquots.
    Summary · context
    Rest three aliquots were diluted with extender containing 2 (Group II), 5 (Group III) and 10 μM Humanin (Group IV), respectively.
  8. 08LabEx vivo tissueEfficacy outcome

    In a homologous zona binding assay, buffalo spermatozoa frozen with 5 µM humanin bound to the zona pellucida in higher numbers than control.

    In plain wordsIn lab tests, buffalo sperm frozen with humanin at 5 µM bound to the egg coat in higher numbers than control.

    Peptide
    Humanin
    Outcome
    IncreaseSpermatozoa bound to zona pellucida
    Study

    buffalon: not reported in source

    buffalo semen frozen in egg yolk-tris-glycerol extender
    Comparator
    Control
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    5 µM · added to semen extenderAs given in this study (buffalo).

    Source sentences

    Summary
    The mean number of spermatozoa bound to zona pellucida was higher (p < 0.05) in 5 μM Humanin treated group than the control group.
    3 context sentences
    Summary · context
    The treatment group that showed best results (5 μM) was compared with control group for in vitro fertility assessment by homologous zona binding assay.
    Summary · context
    For supplementation studies, a total of 24 ejaculates (IPM ≥70%) were collected and each ejaculate was then divided into four aliquots.
    Summary · context
    Rest three aliquots were diluted with extender containing 2 (Group II), 5 (Group III) and 10 μM Humanin (Group IV), respectively.

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 09AnimalEndogenous levelAnimal study (in vivo)Other

    In buffalo ejaculates, seminal plasma humanin levels were significantly and positively correlated with sperm concentration.

    In plain wordsIn buffalo bulls, higher natural humanin levels in semen were linked to higher sperm concentration.

    Peptide
    Humanin
    Outcome
    Not applicableSeminal plasma humanin and sperm concentration
    Study

    buffalon = 32

    buffalo seminal plasma
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    Humanin levels in seminal plasma were significantly and positively correlated with sperm concentration and individual progressive motility (IPM) in good (n = 22; IPM >70%) and poor (n = 10; IPM <50%) quality ejaculates.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  2. 10AnimalEndogenous levelAnimal study (in vivo)Other

    In buffalo ejaculates, seminal plasma humanin levels were significantly and positively correlated with individual progressive motility.

    In plain wordsIn buffalo bulls, higher natural humanin levels in semen were linked to better sperm motility.

    Peptide
    Humanin
    Outcome
    Not applicableSeminal plasma humanin and individual progressive motility
    Study

    buffalon = 32

    buffalo seminal plasma
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    Humanin levels in seminal plasma were significantly and positively correlated with sperm concentration and individual progressive motility (IPM) in good (n = 22; IPM >70%) and poor (n = 10; IPM <50%) quality ejaculates.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

  3. 11AnimalEndogenous levelAnimal study (in vivo)Other

    In buffalo bulls, humanin was present in ejaculated and epididymal spermatozoa, elongated spermatids and interstitial space of testicular tissue.

    In plain wordsIn buffalo bulls, the body's own humanin was found in sperm and testis tissue.

    Peptide
    Humanin
    Outcome
    Not applicablePresence of humanin in spermatozoa and testis
    Study

    buffalon: not reported in source

    buffalo spermatozoa and testicular tissue
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health

    Source sentences

    Summary
    Indirect immunofluorescence test revealed presence of Humanin in ejaculated and epididymal spermatozoa, and, elongated spermatids and interstitial space in the testicular tissue section.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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