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Thymogen (oglufanide) / Studies
Synthetic analog · 2 amino acids · 333.3 Da

Thymogen (oglufanide) studies and research data

PepBank has 69 Thymogen studies with results. 40 of them gave Thymogen, with 123 results, grouped by what the studies measured. In each group, results from studies in people come first. Data as of 2026-10-30.

Searches 123 results and 162 papers. Newer studies from Europe PMC

Results in people
25
20% of 123 results
Results in animals
72
59% of 123 results
Results in lab cells
26
21% of 123 results
From computer models
0
0% of 123 results
The molecule

Thymogen identity

Identifiers, parent molecule, targets and development stage, from curated data.

Thymogen is synthetic alpha-L-glutamyl-L-tryptophan, the dipeptide EW. Oglufanide is the corresponding INN for the defined free dipeptide. The checked Russian Timogen nasal product uses its sodium form. The manufacturer lists nasal spray indications for prevention and combined treatment of infections with reduced immunity. The free dipeptide structure does not include the sodium counterion. Thymalin and thymodepressin are different products.

Kind
Synthetic analog · 2 residues
Identifiers
ChEMBLCHEMBL2111029 (opens in a new tab)PubChem CID100094 (opens in a new tab)UNII4RHY598T5U (opens in a new tab)DrugBankDB05779 (opens in a new tab)CAS38101-59-6
Development
Approved in Russia only (registered as Timogen / Тимоген)Cytomed (Russian Timogen nasal product)The manufacturer supplies a Russian product instruction and identifies Cytomed as the registration holder and manufacturer. PubChem records Russian pharmaceutical registration. The approved stage refers to this reported Russian product status. ChEMBL records maximum phase 3 for free oglufanide. Current registration and label version remain unconfirmed. Source (opens in a new tab)

Targets

No molecular target is recorded for this peptide.

Sequence and structure

Residues, differences from the parent, calculated properties and 3D structure. Source values and calculated values are shown separately.

Sequence

2 amino acids

H-
Residues 1 to 2:
EW
-OH
  • AromaticF W Y
  • NegativeD E

Properties

From identity sources

Formula
C16H19N3O5
Molecular weight
333.34 g/mol
Monoisotopic mass
333.1325 Da

Calculated from the sequence

Average mass
333.34 Da
Sum of ExPASy residue masses + water · ExPASy Compute pI/Mw; FindMod
Monoisotopic mass
333.1325 Da
Sum of ExPASy residue masses + water · ExPASy FindMod
Isoelectric point (pI)
3.85
pH of zero net charge, EMBOSS pKa set · EMBOSS iep, Epk.dat
Net charge at pH 7.4
−1.06
Henderson-Hasselbalch, EMBOSS pKa set · EMBOSS iep, Epk.dat
GRAVY (hydropathy)
−2.200
Mean Kyte-Doolittle hydropathy · Kyte and Doolittle 1982
Negative: hydrophilic on average.
Extinction coefficient, 280 nm (Cys reduced)
5,500 M⁻¹ cm⁻¹
5500 x Trp + 1490 x Tyr · Pace et al. 1995 (ExPASy ProtParam)
Instability index
−70.15
Dipeptide instability weights (DIWV) · Guruprasad et al. 1990
40 or below: classed as stable (ExPASy).
Aliphatic index
0.0
Mole % A + 2.9 V + 3.9 (I + L) · Ikai 1980
Boman index
2.24 kcal/mol
Mean solubility value (negated) · Boman 2003
2.48 or below: lower protein-binding potential (Boman).
Hydrophobic moment (μH)
Not calculated
The sequence is too short for this method.
pI with each pKa set
EMBOSS 3.85Bjellqvist (ExPASy) 4.00IPC peptide 3.35IPC protein 3.64
  • Defined and validated for proteins; use with care for short peptides.
  • Assumes an ideal alpha helix. Many short peptides are not helical.

@pepdb/chem 0.2.0 · EMBOSS pKa set · ExPASy residue masses

Net charge by pH

pI 3.85 · Net charge at pH 7.4: −1.06

Point or use arrow keys

Net charge of Thymogen (oglufanide) from pH 0 to 14 (EMBOSS pKa set). Isoelectric point 3.85. Net charge at pH 7.4: -1.06.−2−10+102468101214pHNet chargepH 7.4pI 3.85

Henderson-Hasselbalch, EMBOSS pKa set. Calculated, not measured.

Show as table
pHNet charge
01.00
11.00
20.97
30.73
4−0.16
5−0.85
6−0.99
7−1.02
8−1.20
9−1.72
10−1.96
11−2.00
12−2.00
13−2.00
14−2.00

Composition

  • Aromatic50% (1)
  • Negative50% (1)

2 residues. Each residue counts in one class; F and W count as aromatic.

A Ala0 · 0%
I Ile0 · 0%
L Leu0 · 0%
M Met0 · 0%
V Val0 · 0%
F Phe0 · 0%
W Trp1 · 50%
Y Tyr0 · 0%
H His0 · 0%
K Lys0 · 0%
R Arg0 · 0%
D Asp0 · 0%
E Glu1 · 50%
N Asn0 · 0%
Q Gln0 · 0%
S Ser0 · 0%
T Thr0 · 0%
C Cys0 · 0%
G Gly0 · 0%
P Pro0 · 0%

3D structure

No 3D structure

No experimental structure (PDB) and no UniProt entry for an AlphaFold model are listed for this peptide. Synthetic peptides often have neither. The sequence view above is the reference.

2D structure

Draws the structure in your browser with RDKit (about 2.3 MB, loaded once).

SMILES fromPubChem CID 100094(opens in a new tab)

C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)N
Results by area

What the studies measured

People first in each area, then animals, then lab cells. Counts are exact and come from studies that gave the peptide.

Trials and papers
Status and safety
Data