Argireline (acetyl hexapeptide-8) / Studies

Synthetic analog · 6 amino acids · 889 Da

Argireline (acetyl hexapeptide-8) studies and research data

PepBank has 33 Argireline studies with results. 19 of them gave Argireline, with 77 results, grouped by what the studies measured. In each group, results from studies in people come first. Data as of 2026-10-30.

Searches 77 results and 49 papers. Newer studies from Europe PMC

Results in people

39% of 77 results

Results in animals

36% of 77 results

Results in lab cells

25% of 77 results

From computer models

0% of 77 results

The molecule

Argireline identity

Identifiers, parent molecule, targets and development stage, from curated data.

Argireline is Ac-EEMQRR-NH2, a six-residue SNAP-25 sequence mimic. GSRS identifies the N-terminal acetyl group and C-terminal argininamide. PubChem CID 11228338 matches this structure despite its acetate title. CID 71587772 has a different formula and is excluded.

Kind: Synthetic analog · 6 residues
Identifiers: UniProtP60880 (opens in a new tab)PubChem CID11228338 (opens in a new tab)UNIIL4EL31FWIL (opens in a new tab)DrugBankDB11709 (opens in a new tab)CAS616204-22-9
Parent: Analog of SNAP-25 · residues 12-17UniProt P60880 residues 12-17 match EEMQRR before terminal modifications.
Development: UnknownA formal clinical development phase was not established.

Targets

No molecular target is recorded for this peptide.

Sequence and structure

Residues, differences from the parent, calculated properties and 3D structure. Source values and calculated values are shown separately.

Sequence

6 amino acids · 2 modifications

EEMQRR

Parent: SNAP-25

No sequence is listed for the parent, so no alignment is shown.UniProt P60880 residues 12-17 match EEMQRR before terminal modifications.

Properties

From identity sources

Formula: C₃₄H₆₀N₁₄O₁₂S
Molecular weight: 889 g/mol
Monoisotopic mass: 888.4236 Da

PubChem CID 11228338(opens in a new tab)retrieved 2026-10-21

Calculated from the sequence

pKa set

Average mass: 889.00 Da; Sum of ExPASy residue masses + water + N-acetyl, C-amide · ExPASy Compute pI/Mw; FindMod
Monoisotopic mass: 888.4236 Da; Sum of ExPASy residue masses + water + N-acetyl, C-amide · ExPASy FindMod
Isoelectric point (pI): 8.30; pH of zero net charge, EMBOSS pKa set · EMBOSS iep, Epk.dat
Net charge at pH 7.4: 0.00; Henderson-Hasselbalch, EMBOSS pKa set · EMBOSS iep, Epk.dat
GRAVY (hydropathy): −2.933; Mean Kyte-Doolittle hydropathy · Kyte and Doolittle 1982; Negative: hydrophilic on average.
Extinction coefficient, 280 nm (Cys reduced): 0 M⁻¹ cm⁻¹; 5500 x Trp + 1490 x Tyr · Pace et al. 1995 (ExPASy ProtParam); No Trp or Tyr: absorbance at 280 nm cannot measure this peptide.
Instability index: 145.57; Dipeptide instability weights (DIWV) · Guruprasad et al. 1990; Above 40: classed as possibly unstable (ExPASy).
Aliphatic index: 0.0; Mole % A + 2.9 V + 3.9 (I + L) · Ikai 1980
Boman index: 7.77 kcal/mol; Mean solubility value (negated) · Boman 2003; Above 2.48: high protein-binding potential (Boman).
Hydrophobic moment (μH): Not calculated; The sequence is too short for this method.
pI with each pKa set: EMBOSS 8.30Bjellqvist (ExPASy) 8.23IPC peptide 8.41IPC protein 8.13

Defined and validated for proteins; use with care for short peptides.
Assumes an ideal alpha helix. Many short peptides are not helical.

@pepdb/chem 0.2.0 · EMBOSS pKa set · ExPASy residue masses

Net charge by pH

pI 8.30 · Net charge at pH 7.4: 0.00

Point or use arrow keys

Henderson-Hasselbalch, EMBOSS pKa set, N-acetyl, C-amide. Calculated, not measured.

Show as table

pH	Net charge
0	2.00
1	2.00
2	1.98
3	1.85
4	1.11
5	0.22
6	0.02
7	0.00
8	0.00
9	−0.00
10	−0.01
11	−0.06
12	−0.48
13	−1.52
14	−1.94

Composition

Hydrophobic17% (1)M
Positive33% (2)R
Negative33% (2)E
Polar17% (1)Q

6 residues. Each residue counts in one class; F and W count as aromatic.

A Ala0 · 0%

I Ile0 · 0%

L Leu0 · 0%

M Met1 · 17%

V Val0 · 0%

F Phe0 · 0%

W Trp0 · 0%

Y Tyr0 · 0%

H His0 · 0%

K Lys0 · 0%

R Arg2 · 33%

D Asp0 · 0%

E Glu2 · 33%

N Asn0 · 0%

Q Gln1 · 17%

S Ser0 · 0%

T Thr0 · 0%

C Cys0 · 0%

G Gly0 · 0%

P Pro0 · 0%

3D structure

PredictedAlphaFold DB · P60880

Loads the 3D viewer (3Dmol.js, about 150 KB) and the model file from AlphaFold DB (EMBL-EBI).

2D structure

Draws the structure in your browser with RDKit (about 2.3 MB, loaded once).

SMILES fromPubChem CID 11228338(opens in a new tab)

CC(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N

Results by area

Argireline results by year and outcome

Counts are exact and come from studies that gave the peptide.

Studies by year

33 of 49 studies have results (67%), published 2002 to 2026. Top row: every study. Bottom row: studies with results about Argireline (acetyl hexapeptide-8), by class. The rows use different scales.

Studies
Human
Animal
Lab
Computer model
Natural levels, tool or combination only

Studies49 in all, 2002 to 2026

Studies with results33 in all, 2002 to 2026

Retracted studies are left out. A study with results in two classes counts in both.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

What the studies measured

People first in each area, then animals, then lab cells. Counts are exact and come from studies that gave the peptide.

Argireline studies on skin, hair and wounds

43 results · people 21 · animals 20 · lab 2

Top 3 of 43 results

In Chinese subjects, argireline improved wrinkles more than placebo in subjective assessments.American journal of clinical dermatology, 2013
In Korean females, microneedle patch/AHP-8 improved wrinkles more than microneedle patch alone.Annals of dermatology, 2019
In healthy volunteers, cR2 change differed between the combination and tripeptide-10 citrulline alone after 20 days.Journal of cosmetic dermatology, 2017

Argireline studies on side effects

10 results · people 5 · animals 2 · lab 3

Top 5 of 10 results

No allergic reactions were documented during acetyl hexapeptide‑8 gelcream treatment in patients with skin disorders.La Clinica terapeutica, 2020
No significant adverse events occurred in the trial of topical acetyl hexapeptide‑8 with botulinum toxin therapy for blepharospasm.European journal of neurology, 2013
In an unspecified animal model, Argireline showed no oral toxicity at high doses.International journal of cosmetic science, 2002
In one woman, redness appeared at argireline injection sites after one week.World journal of clinical cases, 2021
In cells, argireline reduced proliferation in a dose-dependent manner.Acta biochimica Polonica, 2014

Argireline studies on brain and nerves

3 results · people 3 · animals 0 · lab 0

3 results

In blepharospasm patients receiving botulinum toxin, acetyl hexapeptide‑8 tended to extend time to baseline symptom scores versus placebo.European journal of neurology, 2013
In blepharospasm patients receiving botulinum toxin, topical acetyl hexapeptide‑8 tended to improve scores compared with placebo.European journal of neurology, 2013
In the acetyl hexapeptide‑8 group, 4/12 blepharospasm patients had extended symptom control after botulinum toxin, ranging from 3.3-7.1 months.European journal of neurology, 2013

Argireline studies on infections

1 result · people 1 · animals 0 · lab 0

1 result

In one woman with lesions after argireline injections, cultures confirmed Mycobacterium abscessus infection.World journal of clinical cases, 2021

Argireline studies on cells, genes and aging

4 results · people 0 · animals 4 · lab 0

4 results

In zebrafish, ARG reversed the increase in an aging marker after hydrogen peroxide exposure.Journal of cosmetic dermatology, 2025
In zebrafish, ARG counteracted oxidative stress after LPS exposure.Journal of cosmetic dermatology, 2025
In zebrafish, ARG counteracted mitochondrial damage after LPS exposure.Journal of cosmetic dermatology, 2025
In zebrafish, ARG restored telomerase activity after hydrogen peroxide exposure.Journal of cosmetic dermatology, 2025

Argireline studies on immune system and inflammation

2 results · people 0 · animals 2 · lab 0

2 results

In zebrafish, ARG suppressed the increase in neutrophils after LPS exposure.Journal of cosmetic dermatology, 2025
In zebrafish, ARG suppressed the increase in inflammatory cytokines after LPS exposure.Journal of cosmetic dermatology, 2025

Argireline studies on blood levels and clearance

9 results · people 0 · animals 0 · lab 9

Top 3 of 9 results

In lab tests on guinea pig and human skin, most applied acetyl hexapeptide‑8 remained on the surface.Cutaneous and ocular toxicology, 2015
In lab tests on guinea pig skin, the peptide that penetrated the skin remained mostly in the outer skin layer.Cutaneous and ocular toxicology, 2015
In lab tests on human skin, the peptide that penetrated the skin remained mostly in the outer skin layer.Cutaneous and ocular toxicology, 2015

Argireline studies on receptors and cell signals

5 results · people 0 · animals 0 · lab 5

Top 4 of 5 results

In lab tests, Argireline reduced neurotransmitter release.International journal of cosmetic science, 2002
In lab tests, acetyl hexapeptide-3 showed optimal catecholamine content activity at 400 μg/mL.Skin pharmacology and physiology, 2021
In lab tests, Argireline had similar potency to BoNT A for reducing neurotransmitter release.International journal of cosmetic science, 2002
In lab tests, Argireline reduced neurotransmitter release less effectively than BoNT A.International journal of cosmetic science, 2002

Trials and papers

Argireline trials

Registered trials that test the peptide, with phase, status and dates. A dot marks trials with posted results.

StatusPhase

3 trials match. Highest phase first. Data from ClinicalTrials.gov.

Trials on this page (3 of 3)

3 trials · 2 completed · 1 stopped early · 1 with posted results

Completed
Ongoing
Not yet recruiting
Stopped
Estimated dates (est.)
Results posted

2010201220142016

Ph 3
Argireline in Treatment of Periorbital Wrinkles
NCT01381484
Mar 2009 to Sept 2009
Ph 2
Acetyl Hexapeptide-8 for Blepharospasm
NCT01750346
Nov 2012 to May 2015
Terminated
Terminated
N/A
Topical Acetyl Hexapeptide-8 and the Cosmetic Appearance of Oily Skin
NCT02597777
Jan 2016 to Feb 2016

3 trials · 2 completed · 1 stopped early · 1 with posted results · source ClinicalTrials.gov (opens in a new tab)

CompletedPhase 3NCT01381484

Argireline in Treatment of Periorbital Wrinkles

Mahidol Universityn = 70Mar 2009 to Sept 2009

Wrinkles

Trial page ClinicalTrials.govUpdated 27 Jun 2011

TerminatedPhase 2Results postedNCT01750346

Acetyl Hexapeptide-8 for Blepharospasm

National Institute of Neurological Disorders and Stroke (NINDS)n = 8Nov 2012 to May 2015

Focal Dystonia

Trial page ClinicalTrials.govUpdated 10 Nov 2016

CompletedNot applicableNCT02597777

Topical Acetyl Hexapeptide-8 and the Cosmetic Appearance of Oily Skin

University of California, Davisn = 14Jan 2016 to Feb 2016

Oily Skin

Trial page ClinicalTrials.govUpdated 5 Apr 2017

4 more mention it but do not test it. They are not trials of Argireline (acetyl hexapeptide-8): they only measure it (a blood level or an eligibility term) or name it in their text.

Papers

Studies on this peptide. Some have results. The others have none yet.

Sort

Design

49 studies match. Retracted studies are left out.

Showing 24 of 49 studiesquery qry_eceb76956fffaf1fda63c40f

Studies with results

33 studies in all, 18 on this page

Non-randomized trial · 2002

7 results

A synthetic hexapeptide (Argireline) with antiwrinkle activity.

Blanes-Mira et al. 2002International journal of cosmetic sciencecited by 92

PMID 18498523DOI 10.1046/j.1467-2494.2002.00153.xPUB-18498523

Full text at the publisher7 results where Argireline (acetyl hexapeptide-8) was given

Publisher page See results (7)

Not a test of Argireline (acetyl hexapeptide-8) alone · 2021

5 results

High resolution photopolymer for 3D printing of personalised microneedle for transdermal delivery of anti-wrinkle small peptide.

Lim et al. 2021Journal of controlled release : official journal of the Controlled Release Societycited by 73

PMID 33068646DOI 10.1016/j.jconrel.2020.10.021PUB-33068646

Full text at the publisher5 results where Argireline (acetyl hexapeptide-8) was a tool, carrier or part of a combination

Publisher page See results (5)

Not a test of Argireline (acetyl hexapeptide-8) alone · 2020

1 result

Geometrical optimisation of a personalised microneedle eye patch for transdermal delivery of anti-wrinkle small peptide.

Lim et al. 2020Biofabricationcited by 49

PMID 31952064DOI 10.1088/1758-5090/ab6d37PUB-31952064

Full text at the publisher1 result where Argireline (acetyl hexapeptide-8) was a tool, carrier or part of a combination

Publisher page See results (1)

Not a test of Argireline (acetyl hexapeptide-8) alone · 2014

3 results

Enhanced delivery of hydrophilic peptides in vitro by transdermal microneedle pretreatment.

Zhang et al. 2014Acta pharmaceutica Sinica. Bcited by 48

PMID 26579370PMC4590292DOI 10.1016/j.apsb.2013.12.011PUB-26579370

Free to read · publisher termsCC BY-NC-ND2 results where Argireline (acetyl hexapeptide-8) was a tool, carrier or part of a combination

Read at source See results (3)

Randomized controlled trial · 2013

2 results

The anti-wrinkle efficacy of argireline, a synthetic hexapeptide, in Chinese subjects: a randomized, placebo-controlled study.

Wang et al. 2013American journal of clinical dermatologyn = 60cited by 34

PMID 23417317DOI 10.1007/s40257-013-0009-9PUB-23417317

Full text at the publisher2 results where Argireline (acetyl hexapeptide-8) was given

Publisher page See results (2)

Not a test of Argireline (acetyl hexapeptide-8) alone · 2015

4 results

Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.

Hoppel et al. 2015European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciencescited by 30

PMID 25497319DOI 10.1016/j.ejps.2014.12.006PUB-25497319

Full text at the publisher4 results where Argireline (acetyl hexapeptide-8) was a tool, carrier or part of a combination

Publisher page See results (4)

Ex vivo tissue · guinea pig · 2015

9 results

In vitro skin penetration of acetyl hexapeptide-8 from a cosmetic formulation.

Kraeling et al. 2015Cutaneous and ocular toxicologycited by 25

PMID 24754410DOI 10.3109/15569527.2014.894521PUB-24754410

Full text at the publisher9 results where Argireline (acetyl hexapeptide-8) was given

Publisher page See results (9)

Not a test of Argireline (acetyl hexapeptide-8) alone · 2014

6 results

Iontophoretic skin permeation of peptides: an investigation into the influence of molecular properties, iontophoretic conditions and formulation parameters.

Krishnan et al. 2014Drug delivery and translational researchcited by 21

PMID 25786877DOI 10.1007/s13346-013-0181-8PUB-25786877

Rights unknown6 results where Argireline (acetyl hexapeptide-8) was a tool, carrier or part of a combination

Source link See results (6)

Randomized controlled trial · 2019

4 results

Anti-Wrinkle Efficacy of Cross-Linked Hyaluronic Acid-Based Microneedle Patch with Acetyl Hexapeptide-8 and Epidermal Growth Factor on Korean Skin.

An et al. 2019Annals of dermatologycited by 19

PMID 33911590PMC7992733DOI 10.5021/ad.2019.31.3.263PUB-33911590

Free to read · publisher termsCC BY-NC1 result where Argireline (acetyl hexapeptide-8) was given (plus 3 on tools or combinations, listed separately)

Read at source See results (4)

In vitro (cells) · 2014

2 results

The study of cellular cytotoxicity of argireline - an anti-aging peptide.

Grosicki et al. 2014Acta biochimica Polonicacited by 17

PMID 24644551DOI 10.18388/abp.2014_1919PUB-24644551

Full text at the publisher2 results where Argireline (acetyl hexapeptide-8) was given

Publisher page See results (2)

Randomized controlled trial · 2013

4 results

Pilot study of topical acetyl hexapeptide-8 in the treatment for blepharospasm in patients receiving botulinum toxin therapy.

Lungu et al. 2013European journal of neurologyn = 24cited by 16

PMID 23146065PMC4747634DOI 10.1111/ene.12009PUB-23146065

Rights unknown4 results where Argireline (acetyl hexapeptide-8) was given

Source link See results (4)

Animal study (in vivo) · mouse · 2013

3 results

The anti-wrinkle efficacy of Argireline.

Wang et al. 2013Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatologycited by 15

PMID 23464592DOI 10.3109/14764172.2013.769273PUB-23464592

Full text at the publisher3 results where Argireline (acetyl hexapeptide-8) was given

Publisher page See results (3)

Randomized controlled trial · 2017

28 results

The efficacy study of the combination of tripeptide-10-citrulline and acetyl hexapeptide-3. A prospective, randomized controlled study.

Raikou et al. 2017Journal of cosmetic dermatologycited by 15

PMID 28150423DOI 10.1111/jocd.12314PUB-28150423

Full text at the publisher11 results where Argireline (acetyl hexapeptide-8) was given (plus 4 on tools or combinations, listed separately)

Publisher page See results (28)

Not a test of Argireline (acetyl hexapeptide-8) alone · 2024

3 results

Developing Plant Exosomes as an Advanced Delivery System for Cosmetic Peptide.

Hou et al. 2024ACS applied bio materialscited by 12

PMID 38598772DOI 10.1021/acsabm.4c00096PUB-38598772

Full text at the publisher3 results where Argireline (acetyl hexapeptide-8) was a tool, carrier or part of a combination

Publisher page See results (3)

Other design · 2021

14 results

Protective and Anti-Aging Effects of 5 Cosmeceutical Peptide Mixtures on Hydrogen Peroxide-Induced Premature Senescence in Human Skin Fibroblasts.

Wu et al. 2021Skin pharmacology and physiologycited by 10

PMID 33849044DOI 10.1159/000514496PUB-33849044

Full text at the publisher1 result where Argireline (acetyl hexapeptide-8) was given (plus 6 on tools or combinations, listed separately)

Publisher page See results (14)

Not a test of Argireline (acetyl hexapeptide-8) alone · 2020

2 results

Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions.

Hwang et al. 2020Scientific reportscited by 7

PMID 33299102PMC7726138DOI 10.1038/s41598-020-78610-wPUB-33299102

CC BY2 results where Argireline (acetyl hexapeptide-8) was a tool, carrier or part of a combination

Download PDF See results (2)

Case report or series · 2021

4 results

Mycobacterium abscessus infection after facial injection of argireline: A case report.

Chen et al. 2021World journal of clinical casesn = 1cited by 6

PMID 33748252PMC7953399DOI 10.12998/wjcc.v9.i8.1996PUB-33748252

Free to read · publisher termsCC BY-NC4 results where Argireline (acetyl hexapeptide-8) was given

Read at source See results (4)

Animal study (in vivo) · zebrafish · 2025

3 results

Oligomeric hyaluronic acid-modified liposomes effectively improved skin permeability and anti-ageing activity of ellagic acid.

Yang et al. 2025Scientific reportscited by 6

PMID 40715158PMC12297256DOI 10.1038/s41598-025-06948-0PUB-40715158

Free to read · publisher termsCC BY-NC-ND3 results where Argireline (acetyl hexapeptide-8) was given

Read at source See results (3)

More studies

13 studies in all, 6 on this page

Indexing hint: In vitro study · 2018

Corrected

Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification.

Lim et al. 2018Scientific reportscited by 36

PMID 29371611PMC5785486DOI 10.1038/s41598-017-18454-zPUB-29371611

CC BY

Download PDF Read the notice

PubMed tag: none · 2007

Preparation and stability of cosmetic formulations with an anti-aging peptide.

Ruiz et al. 2007Journal of cosmetic sciencecited by 21

PMID 17520155DOI 10.1111/j.1468-2494.2007.00401_4.xPUB-17520155

Full text at the publisher

Publisher page

PubMed tag: Systematic Review, review-article · 2024

Cosmeceuticals in photoaging: A review.

Chan et al. 2024Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)cited by 13

PMID 39233460PMC11375026DOI 10.1111/srt.13730PUB-39233460

CC BY

Download PDF

PubMed tag: Review · 2014

Facial rejuvenation: combining cosmeceuticals with cosmetic procedures.

Wisniewski et al. 2014Cutiscited by 7

PMID 25279473PUB-25279473

Full text at the publisher

Publisher page

PubMed tag: none · 2021

Argireline: Needle-Free Botox as Analytical Challenge.

Kluczyk et al. 2021Chemistry & biodiversitycited by 6

PMID 33482052DOI 10.1002/cbdv.202000992PUB-33482052

Full text at the publisher

Publisher page

PubMed tag: none · 2024

Public Interest in Acetyl Hexapeptide-8: Longitudinal Analysis.

Olsson et al. 2024JMIR dermatologycited by 6

PMID 38376906PMC10915729DOI 10.2196/54217PUB-38376906

CC BY

Download PDF

No result for Argireline (acetyl hexapeptide-8) (1 study on this page, 3 in all)

These studies name Argireline (acetyl hexapeptide-8) but give no result for it (for example, the results concern another peptide in the study).

PubMed tag: none · 2021

3D-bioengineered model of human skeletal muscle tissue with phenotypic features of aging for drug testing purposes.

Mestre et al. 2021Biofabricationcited by 18

PMID 34284359DOI 10.1088/1758-5090/ac165bPUB-34284359

Rights unknown

Source link

Newer studies

PepBank reads each paper before it shows the results. This search looks in Europe PMC for newer papers that PepBank has not read yet.

Argireline (acetyl hexapeptide-8) studies: 33 papers with results

Page 1 of 1

Pilot study of topical acetyl hexapeptide-8 in the treatment for blepharospasm in patients receiving botulinum toxin therapy.
Lungu C · European journal of neurology · 2013
Randomized controlled trial · 4 results
The anti-wrinkle efficacy of argireline, a synthetic hexapeptide, in Chinese subjects: a randomized, placebo-controlled study.
Wang Y · American journal of clinical dermatology · 2013
Randomized controlled trial · 2 results
Anti-Wrinkle Efficacy of Cross-Linked Hyaluronic Acid-Based Microneedle Patch with Acetyl Hexapeptide-8 and Epidermal Growth Factor on Korean Skin.
An JH · Annals of dermatology · 2019
Randomized controlled trial · 4 results
The efficacy study of the combination of tripeptide-10-citrulline and acetyl hexapeptide-3. A prospective, randomized controlled study.
Raikou V · Journal of cosmetic dermatology · 2017
Randomized controlled trial · 28 results
The anti wrinkle efficacy of synthetic hexapeptide (Argireline) in Chinese Subjects.
Wang Y · Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology · 2013
Randomized controlled trial · 5 results
Investigating the effects of Argireline in a skin serum containing hyaluronic acids on skin surface wrinkles using the Visia® Complexion Analysis camera system for objective skin analysis.
Henseler H · GMS Interdisciplinary plastic and reconstructive surgery DGPW · 2023
Non-randomized trial · 5 results
A synthetic hexapeptide (Argireline) with antiwrinkle activity.
Blanes-Mira C · International journal of cosmetic science · 2002
Non-randomized trial · 7 results
Skin scars and wrinkles temporary camouflage in dermatology and oncoesthetics: focus on acetyl hexapeptide-8
Palmieri B · La Clinica terapeutica · 2020
Observational study · 3 results
Mycobacterium abscessus infection after facial injection of argireline: A case report.
Chen CF · World journal of clinical cases · 2021
Case report or series · 4 results
Effects of Botulinum Toxin Type A and Argireline on Dermal Collagen Remodeling and Skin Biology in a Flap Model.
Acuner B · Aesthetic plastic surgery · 2026
Animal study (in vivo) · rat · 9 results
The anti-wrinkle efficacy of Argireline.
Wang Y · Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology · 2013
Animal study (in vivo) · mouse · 3 results
Oligomeric hyaluronic acid-modified liposomes effectively improved skin permeability and anti-ageing activity of ellagic acid.
Yang X · Scientific reports · 2025
Animal study (in vivo) · zebrafish · 3 results
BAK and ARG Have Moisturizing, Anti-Inflammatory, Antioxidant, and Antioxidant Bioactivities, in the Zebrafish Model.
Jiang X · Journal of cosmetic dermatology · 2025
Animal study (in vivo) · danio rerio · 10 results
Thermostable hyaluronic acid-based dissolving microneedles with high-loading capacity: design, optimization, and transdermal delivery of anti-aging ingredients.
Feng M · International journal of biological macromolecules · 2026
Animal study (in vivo) · mouse · 8 results
In vitro skin penetration of acetyl hexapeptide-8 from a cosmetic formulation.
Kraeling ME · Cutaneous and ocular toxicology · 2015
Ex vivo tissue · guinea pig · 9 results
The study of cellular cytotoxicity of argireline - an anti-aging peptide.
Grosicki M · Acta biochimica Polonica · 2014
In vitro (cells) · 2 results
Mechanistic understanding and peptide ingredient screening for type III collagen via biological knowledge graph and molecular docking.
Zang S · International journal of cosmetic science · 2026
In vitro (cells) · 18 results
Cosmetic potential of Ganoderma lucidum (Reishi) extract in a topical cream formulation.
Dikmen Kucuk S · International journal of cosmetic science · 2026
In vitro (cells) · 1 result
Protective and Anti-Aging Effects of 5 Cosmeceutical Peptide Mixtures on Hydrogen Peroxide-Induced Premature Senescence in Human Skin Fibroblasts.
Wu Y · Skin pharmacology and physiology · 2021
Other design · 14 results
Ex Vivo Evaluation of Skin Permeability Enhancement Using TargetCool in Human-Derived Skin Tissue Models.
Yi KH · The Journal of craniofacial surgery · 2026
Not a test of Argireline (acetyl hexapeptide-8) alone · 11 results
The effect of a serum containing acetyl hexapeptide-8, dipeptide diaminobutyroyl benzylamide diacetate and gluconolactone on skin biomarkers, wrinkles and skin texture: Ex vivo and clinical studies.
Zhu M · International journal of cosmetic science · 2026
Not a test of Argireline (acetyl hexapeptide-8) alone · 17 results
Dual-function supramolecular system of α-hydroxy acid-based ionic liquids and peptides for enhanced anti-aging transdermal delivery.
Wang Z · Journal of materials chemistry. B · 2025
Not a test of Argireline (acetyl hexapeptide-8) alone · 7 results
Iontophoretic skin permeation of peptides: an investigation into the influence of molecular properties, iontophoretic conditions and formulation parameters.
Krishnan G · Drug delivery and translational research · 2014
Not a test of Argireline (acetyl hexapeptide-8) alone · 6 results
High resolution photopolymer for 3D printing of personalised microneedle for transdermal delivery of anti-wrinkle small peptide.
Lim SH · Journal of controlled release : official journal of the Controlled Release Society · 2021
Not a test of Argireline (acetyl hexapeptide-8) alone · 5 results
A Supramolecular System of Bioactive Ionic Liquid, Peony Extract, and Peptide for Enhanced Permeability and Synergistic Skincare Benefits.
Wang M · Chemistry (Weinheim an der Bergstrasse, Germany) · 2025
Not a test of Argireline (acetyl hexapeptide-8) alone · 5 results
Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.
Hoppel M · European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences · 2015
Not a test of Argireline (acetyl hexapeptide-8) alone · 4 results
Polydioxanone Bioactive Sutures-Acetyl Hexapeptide-8 (Argireline): An Intelligent System for Controlled Release in Facial Harmonization.
Velazco de Maldonado GJ · Journal of cutaneous and aesthetic surgery · 2023
Not a test of Argireline (acetyl hexapeptide-8) alone · 4 results
DES-mediated self-assembled polypeptides: Synergistic neuromuscular signaling inhibition for anti-aging.
Bai · Biomaterials advances · 2026
Not a test of Argireline (acetyl hexapeptide-8) alone · 4 results
Developing Plant Exosomes as an Advanced Delivery System for Cosmetic Peptide.
Hou J · ACS applied bio materials · 2024
Not a test of Argireline (acetyl hexapeptide-8) alone · 3 results
Enhanced delivery of hydrophilic peptides in vitro by transdermal microneedle pretreatment.
Zhang S · Acta pharmaceutica Sinica. B · 2014
Not a test of Argireline (acetyl hexapeptide-8) alone · 3 results
Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions.
Hwang W · Scientific reports · 2020
Not a test of Argireline (acetyl hexapeptide-8) alone · 2 results
Geometrical optimisation of a personalised microneedle eye patch for transdermal delivery of anti-wrinkle small peptide.
Lim SH · Biofabrication · 2020
Not a test of Argireline (acetyl hexapeptide-8) alone · 1 result
Fluorous oligoarginines as supra-enhancers for intracellular and transdermal peptide delivery.
Rong G · Bioactive materials · 2026
Not a test of Argireline (acetyl hexapeptide-8) alone · 1 result

Status and safety

Regulatory and safety

Approval and sport status, and the safety outcomes reported in study results. Laws and labels change, so check the regulator for the current status.

Regulatory status by jurisdiction, with date and source
Jurisdiction	Body	Status	As of	Source
US	FDA	Cosmetic ingredient, not an FDA-approved drug. Cosmetic marketing does not establish therapeutic approval.	22 Oct 2026	fda.gov (opens in a new tab)
EU	EMA	No approved medicinal use was established. A CosIng inventory entry is not medicinal approval. EU national medicine records remain outside this check.	22 Oct 2026	ec.europa.eu (opens in a new tab)
Sport	WADA	Status unknown Not specifically named.	2026 List	wada-ama.org (opens in a new tab)
EU cosmetics	Note	INCI: Acetyl Hexapeptide-8. CosIng reference 54135. The public entry did not return its full text through the reader. Inventory inclusion is not authorisation. The European Commission says inclusion in CosIng is not an indication of authorisation (CosIng home page, accessed 2026-10-22). It is not approved as a medicine.	22 Oct 2026	ec.europa.eu (opens in a new tab)

Safety outcomes

11 safety results for Argireline (acetyl hexapeptide-8).

Human evidence· 6

No significant adverse events occurred in the blepharospasm trial of topical acetyl hexapeptide‑8 with botulinum toxin therapy.
Randomized controlled trialn = 24
No allergic reactions were documented during acetyl hexapeptide‑8 gelcream treatment in patients with skin disorders.
Observational studyn = 26
In a woman, erythema appeared at argireline injection sites after one week.
Case report or seriesn = 1
In a woman, nodules appeared at argireline injection sites after one week.
Case report or seriesn = 1
In a woman, abscesses appeared at argireline injection sites after one week.
Case report or seriesn = 1
In a woman with lesions after argireline injections, pus cultures confirmed Mycobacterium abscessus infection.
Case report or seriesn = 1

Animal evidence· 2

In an unspecified in vivo model, Argireline did not exhibit oral toxicity at high doses.
Other design
In an unspecified in vivo model, Argireline did not exhibit primary irritation at high doses.
Other design

Lab evidence· 3

In human cell cultures, significant argireline cytotoxicity required concentrations 18 to 10 000 fold higher than doxorubicin, depending on cell type.
In vitro (cells)human
Cytotoxic concentration ratio
18 to 10,000 fold
argireline versus doxorubicin, depending on cells
In human dermal fibroblasts, acetyl hexapeptide‑8 showed no detectable cytotoxicity.
In vitro (cells)human
In HaCaT keratinocytes, argireline exhibited cytotoxicity at lower concentrations than Reishi extract.
In vitro (cells)human

Doses studied · 4

The doses that these studies used. They are not recommendations.

10%Human
Species
human
Route
topical
Duration
30 days
Design
Non-randomized trial
10%Human
Species
human
Route
topical
Duration
Not reported
Design
Observational study · n = 26
10% at 2 mg/cm(2)Lab
Species
guinea pig and human
Route
topical
Duration
24 h
Design
Ex vivo tissue
400 μg/mLLab
Species
Not reported
Route
Not reported
Duration
Not reported
Design
Other design

Doses studied in the study results, as given in the studies.
Dose as reported	Class	Species	Route	Duration	Design
10%	Human	human	topical	30 days	Non-randomized trial
10%	Human	human	topical	Not reported	Observational study · n = 26
10% at 2 mg/cm(2)	Lab	guinea pig and human	topical	24 h	Ex vivo tissue
400 μg/mL	Lab	Not reported	Not reported	Not reported	Other design

PepBank is a research reference. Talk to a doctor before you use any peptide.

Data

Data and API

Data version, change history and code for this peptide.

Data version 30856030 Oct 2026

Peptide

Peptide id: PEP-000104
Peptide version: 3
Peptide hash: 3b105c5553fe3b3a…
Data version: 308560

Latest changes

27 Oct 2026UpdatedData version 256642
22 Oct 2026UpdatedData version 254491
21 Oct 2026CreatedData version 228093

For developers

curl -s https://pepbank.io/v1/briefs/argireline

Evidence summary for Argireline (acetyl hexapeptide-8): counts by class, key results with sources, notices, data version.

Peptide as JSON Evidence summary as JSON

Outcome group	Human	Animal	Lab	Computer model	Total
Skin and hairSkin and wound	Human: 21 results from 7 studies: 3 increase, 4 decrease, 2 no significant change, 12 not reported. Strongest design: Randomized controlled trial	Animal: 20 results from 6 studies: 9 increase, 9 decrease, 2 no significant change. Strongest design: Animal study (in vivo)	Lab: 2 results from 1 study: 1 increase, 1 no significant change. Strongest design: In vitro (cells)	No results	43
Adverse eventsWhole body	Human: 5 results from 3 studies: 5 not reported. Strongest design: Randomized controlled trial	Animal: 2 results from 1 study: 2 no significant change. Strongest design: Other design	Lab: 3 results from 2 studies: 1 increase, 2 decrease. Strongest design: In vitro (cells)	No results	10
Locomotor and behavioral activityEye	Human: 3 results from 1 study: 1 increase, 2 not reported. Strongest design: Randomized controlled trial	No results	No results	No results	3
Infection and antimicrobial activityInfection	Human: 1 result from 1 study: 1 not reported. Strongest design: Case report or series	No results	No results	No results	1
PharmacokineticsPharmacology	No results	No results	Lab: 9 results from 1 study: 1 decrease, 8 not reported. Strongest design: Ex vivo tissue	No results	9
Receptor activity and mechanismSkin and wound	No results	No results	Lab: 5 results from 2 studies: 2 decrease, 3 not reported. Strongest design: In vitro (cells)	No results	5
Aging markersWhole body	No results	Animal: 3 results from 1 study: 1 increase, 2 decrease. Strongest design: Animal study (in vivo)	No results	No results	3
Immune responseImmune and inflammation	No results	Animal: 1 result from 1 study: 1 decrease. Strongest design: Animal study (in vivo)	No results	No results	1
Inflammation markersImmune and inflammation	No results	Animal: 1 result from 1 study: 1 decrease. Strongest design: Animal study (in vivo)	No results	No results	1
Mitochondrial functionWhole body	No results	Animal: 1 result from 1 study: 1 decrease. Strongest design: Animal study (in vivo)	No results	No results	1

Outcome group	Human	Animal	Lab	Computer model	Total
Skin and hairSkin and wound	Human: 21 results from 7 studies: 3 increase, 4 decrease, 2 no significant change, 12 not reported. Strongest design: Randomized controlled trial	Animal: 20 results from 6 studies: 9 increase, 9 decrease, 2 no significant change. Strongest design: Animal study (in vivo)	Lab: 2 results from 1 study: 1 increase, 1 no significant change. Strongest design: In vitro (cells)	No results	43
Adverse eventsWhole body	Human: 5 results from 3 studies: 5 not reported. Strongest design: Randomized controlled trial	Animal: 2 results from 1 study: 2 no significant change. Strongest design: Other design	Lab: 3 results from 2 studies: 1 increase, 2 decrease. Strongest design: In vitro (cells)	No results	10
Locomotor and behavioral activityEye	Human: 3 results from 1 study: 1 increase, 2 not reported. Strongest design: Randomized controlled trial	No results	No results	No results	3
Infection and antimicrobial activityInfection	Human: 1 result from 1 study: 1 not reported. Strongest design: Case report or series	No results	No results	No results	1
PharmacokineticsPharmacology	No results	No results	Lab: 9 results from 1 study: 1 decrease, 8 not reported. Strongest design: Ex vivo tissue	No results	9
Receptor activity and mechanismSkin and wound	No results	No results	Lab: 5 results from 2 studies: 2 decrease, 3 not reported. Strongest design: In vitro (cells)	No results	5
Aging markersWhole body	No results	Animal: 3 results from 1 study: 1 increase, 2 decrease. Strongest design: Animal study (in vivo)	No results	No results	3
Immune responseImmune and inflammation	No results	Animal: 1 result from 1 study: 1 decrease. Strongest design: Animal study (in vivo)	No results	No results	1
Inflammation markersImmune and inflammation	No results	Animal: 1 result from 1 study: 1 decrease. Strongest design: Animal study (in vivo)	No results	No results	1
Mitochondrial functionWhole body	No results	Animal: 1 result from 1 study: 1 decrease. Strongest design: Animal study (in vivo)	No results	No results	1

Argireline (acetyl hexapeptide-8) studies and research data

Argireline identity

Targets

Sequence and structure

Sequence

Parent: SNAP-25

Properties

Net charge by pH

Composition

3D structure

2D structure

Argireline results by year and outcome

What the studies measured

Argireline studies on skin, hair and wounds

Argireline studies on side effects

Argireline studies on brain and nerves

Argireline studies on infections

Argireline studies on cells, genes and aging

Argireline studies on immune system and inflammation

Argireline studies on blood levels and clearance

Argireline studies on receptors and cell signals

Argireline trials

Argireline in Treatment of Periorbital Wrinkles

Acetyl Hexapeptide-8 for Blepharospasm

Topical Acetyl Hexapeptide-8 and the Cosmetic Appearance of Oily Skin

Papers

Studies with results

A synthetic hexapeptide (Argireline) with antiwrinkle activity.

High resolution photopolymer for 3D printing of personalised microneedle for transdermal delivery of anti-wrinkle small peptide.

Geometrical optimisation of a personalised microneedle eye patch for transdermal delivery of anti-wrinkle small peptide.

Enhanced delivery of hydrophilic peptides in vitro by transdermal microneedle pretreatment.

The anti-wrinkle efficacy of argireline, a synthetic hexapeptide, in Chinese subjects: a randomized, placebo-controlled study.

Topical delivery of acetyl hexapeptide-8 from different emulsions: influence of emulsion composition and internal structure.

In vitro skin penetration of acetyl hexapeptide-8 from a cosmetic formulation.

Iontophoretic skin permeation of peptides: an investigation into the influence of molecular properties, iontophoretic conditions and formulation parameters.

Anti-Wrinkle Efficacy of Cross-Linked Hyaluronic Acid-Based Microneedle Patch with Acetyl Hexapeptide-8 and Epidermal Growth Factor on Korean Skin.

The study of cellular cytotoxicity of argireline - an anti-aging peptide.

Pilot study of topical acetyl hexapeptide-8 in the treatment for blepharospasm in patients receiving botulinum toxin therapy.

The anti-wrinkle efficacy of Argireline.

The efficacy study of the combination of tripeptide-10-citrulline and acetyl hexapeptide-3. A prospective, randomized controlled study.

Developing Plant Exosomes as an Advanced Delivery System for Cosmetic Peptide.

Protective and Anti-Aging Effects of 5 Cosmeceutical Peptide Mixtures on Hydrogen Peroxide-Induced Premature Senescence in Human Skin Fibroblasts.

Topical application of Zanthoxylum piperitum extract improves lateral canthal rhytides by inhibiting muscle contractions.

Mycobacterium abscessus infection after facial injection of argireline: A case report.

Oligomeric hyaluronic acid-modified liposomes effectively improved skin permeability and anti-ageing activity of ellagic acid.

More studies

Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification.

Preparation and stability of cosmetic formulations with an anti-aging peptide.

Cosmeceuticals in photoaging: A review.

Facial rejuvenation: combining cosmeceuticals with cosmetic procedures.

Argireline: Needle-Free Botox as Analytical Challenge.

Public Interest in Acetyl Hexapeptide-8: Longitudinal Analysis.

3D-bioengineered model of human skeletal muscle tissue with phenotypic features of aging for drug testing purposes.

Newer studies

Argireline (acetyl hexapeptide-8) studies: 33 papers with results

Regulatory and safety

Human evidence· 6

Animal evidence· 2

Lab evidence· 3

Doses studied · 4

Data and API

Peptide

Latest changes

For developers

Related peptides

Argireline results by year and outcome

Argireline studies on skin, hair and wounds

Argireline studies on side effects

Argireline studies on brain and nerves

Argireline studies on infections

Argireline studies on cells, genes and aging

Argireline studies on immune system and inflammation

Argireline studies on blood levels and clearance

Argireline studies on receptors and cell signals

Argireline trials

Argireline in Treatment of Periorbital Wrinkles

Acetyl Hexapeptide-8 for Blepharospasm

Topical Acetyl Hexapeptide-8 and the Cosmetic Appearance of Oily Skin

Papers

Studies with results