BAK and ARG Have Moisturizing, Anti-Inflammatory, Antioxidant, and Antioxidant Bioactivities, in the Zebrafish Model.

Jiang X, Wang W, Wei Y, Cheng W, Li S, Du L, Xu W, Zhang Y

Journal of cosmetic dermatology1 Apr 2025Cited by 3 (Europe PMC)

PMID40176351PMCIDPMC11965963DOI10.1111/jocd.70128PepBankPUB-40176351

Summary

BackgroundBakuchiol (BAK) and acetyl hexapeptide-8 have favorable biological activities and have promising applications in the cosmetic industry. However, the evaluation of the biological activity of both alone or in combination has not been reported.AimsThe biological activity of both alone or in combination was evaluated based on the zebrafish model.MethodsZebrafish embryos were induced to form models using different methods. Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.ResultsBAK and ARG were able to reduce water loss from the caudal fin of zebrafish induced by 0.9% NaCl solution. They were able to alleviate the UVB-induced decrease in the expression level of the skin tightness-related gene (ELN/COL1a1b) in zebrafish embryos, and BAK or ARG effectively reversed the increase in β-galactosidase activity induced by exposure to H₂O₂ solution and restored telomerase activity in zebrafish embryos. In addition, both were able to counteract oxidative stress and mitochondrial damage in zebrafish embryos as a result of LPS treatment. Finally, BAK and ARG were also effective in suppressing the increase in neutrophil counts and inflammatory cytokine levels in zebrafish embryos due to LPS exposure. Notably, BAK and ARG were more effective when used in combination.ConclusionsAcetyl hexapeptide-8 promotes the bioactivity of BAK in zebrafish embryos (Danio rerio). BAK and ARG have moisturizing, anti-inflammatory, antioxidant, and antioxidant bioactivities in the zebrafish model.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

9 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish, ARG reduced caudal fin water loss induced by NaCl.
In plain wordsIn zebrafish, ARG reduced salt-induced water loss.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreaseCaudal fin water loss
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated
Source sentences
Summary
BAK and ARG were able to reduce water loss from the caudal fin of zebrafish induced by 0.9% NaCl solution.
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635101
02AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, ARG alleviated the UVB-induced reduction in ELN expression.
In plain wordsIn zebrafish, ARG reduced the loss of ELN gene expression after UVB exposure.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreaseELN expression
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated
Source sentences
Summary
They were able to alleviate the UVB-induced decrease in the expression level of the skin tightness-related gene (ELN/COL1a1b) in zebrafish embryos,
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635102
03AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, ARG reversed the hydrogen peroxide-induced increase in β-galactosidase activity.
In plain wordsIn zebrafish, ARG reversed the increase in an aging marker after hydrogen peroxide exposure.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Decreaseβ-galactosidase activity
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Aging markers · Aging and longevity
Studied dose
Dose not stated
Source sentences
Summary
and BAK or ARG effectively reversed the increase in β-galactosidase activity induced by exposure to H₂O₂ solution and restored telomerase activity in zebrafish embryos.
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635103
04AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, ARG restored telomerase activity after hydrogen peroxide exposure.
In plain wordsIn zebrafish, ARG restored telomerase activity after hydrogen peroxide exposure.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreaseTelomerase activity
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Aging markers · Aging and longevity
Studied dose
Dose not stated
Source sentences
Summary
and BAK or ARG effectively reversed the increase in β-galactosidase activity induced by exposure to H₂O₂ solution and restored telomerase activity in zebrafish embryos.
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635104
05AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, ARG counteracted LPS-induced oxidative stress.
In plain wordsIn zebrafish, ARG counteracted oxidative stress after LPS exposure.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreaseOxidative stress
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Aging markers · Whole body
Studied dose
Dose not stated
Source sentences
Summary
In addition, both were able to counteract oxidative stress and mitochondrial damage in zebrafish embryos as a result of LPS treatment.
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635105
06AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, ARG counteracted LPS-induced mitochondrial damage.
In plain wordsIn zebrafish, ARG counteracted mitochondrial damage after LPS exposure.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreaseMitochondrial damage
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Mitochondrial function · Whole body
Studied dose
Dose not stated
Source sentences
Summary
In addition, both were able to counteract oxidative stress and mitochondrial damage in zebrafish embryos as a result of LPS treatment.
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635106
07AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, ARG suppressed the LPS-induced increase in neutrophil counts.
In plain wordsIn zebrafish, ARG suppressed the increase in neutrophils after LPS exposure.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreaseNeutrophil counts
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Immune response · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Finally, BAK and ARG were also effective in suppressing the increase in neutrophil counts and inflammatory cytokine levels in zebrafish embryos due to LPS exposure.
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635107
08AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, ARG suppressed the LPS-induced increase in inflammatory cytokine levels.
In plain wordsIn zebrafish, ARG suppressed the increase in inflammatory cytokines after LPS exposure.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreaseInflammatory cytokine levels
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Finally, BAK and ARG were also effective in suppressing the increase in neutrophil counts and inflammatory cytokine levels in zebrafish embryos due to LPS exposure.
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635108
09AnimalAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, ARG alleviated the UVB-induced reduction in COL1a1b expression.
In plain wordsIn zebrafish, ARG reduced the loss of COL1a1b gene expression after UVB exposure.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreaseCOL1a1b expression
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
induced damage
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated
Source sentences
Summary
They were able to alleviate the UVB-induced decrease in the expression level of the skin tightness-related gene (ELN/COL1a1b) in zebrafish embryos,
1 context sentence(design, population, supporting numbers)
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
Show in the study text Report a problemFND-4017635110

Studies that did not test the peptide alone1

In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Argireline (acetyl hexapeptide-8).

10AnimalNot a test of Argireline (acetyl hexapeptide-8) aloneAnimal study (in vivo)Efficacy outcome
In zebrafish embryos, combined BAK and ARG showed greater effectiveness than either agent alone, without outcome-specific estimates.
In plain wordsIn zebrafish, BAK and ARG together were more effective than either alone, without separate outcome estimates.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreaseCombined biological activity
Study
Danio rerion: not reported in source
zebrafish embryos
Comparator
BAK or ARG alone
Outcome group
Skin and hair · Skin and wound
Source sentences
Summary
Notably, BAK and ARG were more effective when used in combination.
2 context sentences(design, population, supporting numbers)
Summary · context
The biological activity of both alone or in combination was evaluated based on the zebrafish model.
Summary · context
Add BAK and Argireline YOUth peptide oil solution MB (ARG, containing 0.125% acetyl hexapeptide-8) to treat zebrafish embryos, and they were evaluated for their restorative effects on preexisting damage in zebrafish embryos.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Show in the study text Report a problemFND-4017635109

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
22 Oct 2026, 02:25 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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