Effects of Botulinum Toxin Type A and Argireline on Dermal Collagen Remodeling and Skin Biology in a Flap Model.

Acuner B, Görgülü T, Işıktekin E, Kaymaz Morkoç E, Kargı AE

Aesthetic plastic surgery31 Aug 2026

PMID42675285DOI10.1007/s00266-026-06225-zPepBankPUB-42675285

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

2 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In rats, flap survival did not differ significantly among Argireline, saline, BoNT-A and combination groups.
In plain wordsIn rats, flap survival did not differ significantly among Argireline and the other groups.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
No significant changeFlap survival
Study
ratn = 40
female Wistar rat McFarlane flap
Comparator
Saline, BoNT-A and combination groups
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated · subdermalAs given in this study (rat).
Source sentences
Summary
Flap survival did not differ significantly among groups (p = 0.327).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
Report a problemFND-4267528501
02AnimalAnimal study (in vivo)Efficacy outcome
In rats, Argireline alone produced a collagen profile indistinguishable from saline control.
In plain wordsIn rats, Argireline alone did not change the collagen profile versus saline control.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
No significant changeCollagen profile
Study
ratn = 20
female Wistar rat McFarlane flap
Comparator
Saline control
Outcome group
Skin and hair · Skin and wound
Studied dose
Dose not stated · subdermalAs given in this study (rat).
Source sentences
Summary
Argireline alone produced a collagen profile indistinguishable from control.
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
Report a problemFND-4267528503

Studies that did not test the peptide alone7

In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Argireline (acetyl hexapeptide-8).

03AnimalNot a test of Argireline (acetyl hexapeptide-8) aloneAnimal study (in vivo)Efficacy outcome
In rats, flap survival did not differ significantly among the BoNT-A plus Argireline group and the other groups.
In plain wordsIn rats, flap survival did not differ significantly between the combined treatment and the other groups.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
No significant changeFlap survival with combination
Study
ratn = 40
female Wistar rat McFarlane flap
Comparator
Saline, BoNT-A and Argireline groups
Outcome group
Skin and hair · Skin and wound
Source sentences
Summary
Flap survival did not differ significantly among groups (p = 0.327).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-4267528502
04AnimalNot a test of Argireline (acetyl hexapeptide-8) aloneAnimal study (in vivo)Efficacy outcome
In rats, BoNT-A-containing groups, including BoNT-A plus Argireline, had lower type I collagen than saline control.
In plain wordsIn rats, groups with BoNT-A, including the combination, had less type I collagen than saline control.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreaseType I collagen
Study
ratn = 30
female Wistar rat McFarlane flap
Comparator
Saline control
Outcome group
Skin and hair · Skin and wound
Source sentences
Summary
Collagen composition differed significantly: BoNT-A-containing groups demonstrated lower type I and higher type III collagen compared with control and Argireline groups (p < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-4267528504
05AnimalNot a test of Argireline (acetyl hexapeptide-8) aloneAnimal study (in vivo)Efficacy outcome
In rats, BoNT-A-containing groups, including the combination, had lower type I collagen than Argireline alone.
In plain wordsIn rats, groups with BoNT-A, including the combination, had less type I collagen than Argireline alone.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreaseType I collagen
Study
ratn = 30
female Wistar rat McFarlane flap
Comparator
Argireline alone
Outcome group
Skin and hair · Skin and wound
Source sentences
Summary
Collagen composition differed significantly: BoNT-A-containing groups demonstrated lower type I and higher type III collagen compared with control and Argireline groups (p < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-4267528505
06AnimalNot a test of Argireline (acetyl hexapeptide-8) aloneAnimal study (in vivo)Efficacy outcome
In rats, BoNT-A-containing groups, including the combination, had higher type III collagen than saline control.
In plain wordsIn rats, groups with BoNT-A, including the combination, had more type III collagen than saline control.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreaseType III collagen
Study
ratn = 30
female Wistar rat McFarlane flap
Comparator
Saline control
Outcome group
Skin and hair · Skin and wound
Source sentences
Summary
Collagen composition differed significantly: BoNT-A-containing groups demonstrated lower type I and higher type III collagen compared with control and Argireline groups (p < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-4267528506
07AnimalNot a test of Argireline (acetyl hexapeptide-8) aloneAnimal study (in vivo)Efficacy outcome
In rats, BoNT-A-containing groups, including the combination, had higher type III collagen than Argireline alone.
In plain wordsIn rats, groups with BoNT-A, including the combination, had more type III collagen than Argireline alone.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreaseType III collagen
Study
ratn = 30
female Wistar rat McFarlane flap
Comparator
Argireline alone
Outcome group
Skin and hair · Skin and wound
Source sentences
Summary
Collagen composition differed significantly: BoNT-A-containing groups demonstrated lower type I and higher type III collagen compared with control and Argireline groups (p < 0.001).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-4267528507
08AnimalNot a test of Argireline (acetyl hexapeptide-8) aloneAnimal study (in vivo)Mechanism
In rats, BoNT-A plus Argireline increased Substance P expression versus saline control.
In plain wordsIn rats, BoNT-A plus Argireline increased Substance P expression versus saline control.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreaseSubstance P expression
Study
ratn = 20
female Wistar rat McFarlane flap
Comparator
Saline control
Outcome group
Skin and hair · Skin and wound
Source sentences
Summary
SP expression was significantly elevated in the combination group compared with control and Argireline (p = 0.002).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-4267528508
09AnimalNot a test of Argireline (acetyl hexapeptide-8) aloneAnimal study (in vivo)Mechanism
In rats, BoNT-A plus Argireline increased Substance P expression versus Argireline alone.
In plain wordsIn rats, BoNT-A plus Argireline increased Substance P expression versus Argireline alone.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreaseSubstance P expression
Study
ratn = 20
female Wistar rat McFarlane flap
Comparator
Argireline alone
Outcome group
Skin and hair · Skin and wound
Source sentences
Summary
SP expression was significantly elevated in the combination group compared with control and Argireline (p = 0.002).
2 context sentences(design, population, supporting numbers)
Summary · context
Forty female Wistar rats were allocated to four groups (n = 10): saline control, BoNT-A, Argireline, and BoNT-A + Argireline.
Summary · context
Subdermal injections were administered at six points one week before elevation of a 9 × 3 cm caudally based McFarlane flap.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-4267528509

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
22 Oct 2026, 02:25 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

Keywords

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