Iontophoretic skin permeation of peptides: an investigation into the influence of molecular properties, iontophoretic conditions and formulation parameters.

Krishnan G, Roberts MS, Grice J, Anissimov YG, Moghimi HR, Benson HA

Drug delivery and translational research1 Jun 2014Cited by 21 (Europe PMC)

PMID25786877DOI10.1007/s13346-013-0181-8PepBankPUB-25786877

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Argireline (acetyl hexapeptide-8).

01LabNot a test of Argireline (acetyl hexapeptide-8) aloneEx vivo tissuePharmacokinetics
In excised human skin, iontophoresis increased pooled peptide permeation, including Argireline, up to 30 times versus passive permeation.
In plain wordsIn lab tests on human skin, iontophoresis increased pooled peptide permeation, including Argireline, up to 30 times versus passive delivery.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreasePooled peptide permeation with iontophoresis
Study
humann: not reported in source
Excised human skin
Comparator
Passive permeation
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
Iontophoresis led up to a 30 times increase in peptide permeation relative to passive permeation for the peptides.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, the permeation of alanine-tryptophan dipeptide (MW 276 Da), alanine-alanine-proline-valine tetrapeptide (MW 355 Da), Argireline® (Acetyl hexapeptide-3, MW 889 Da) and Triptorelin acetate (decapeptide, MW 1311 Da) through excised human skin under passive or iontophoretic current of 0.4…
Summary · context
The effects of pH change (3.0-7.4, to provide different net negative, neutral, and positive charges) to the peptide, donor concentration (1-10 mg/ml), background electrolyte (34-137 mM NaCl and/or 5-20 mM HEPES) and current direction (anodal vs cathodal) were also studied.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-2578687701
02LabNot a test of Argireline (acetyl hexapeptide-8) aloneEx vivo tissueMechanism
In excised human skin, electroosmosis importantly determined total flux of high molecular weight charged peptides in the pool containing Argireline.
In plain wordsIn lab tests on human skin, electroosmosis contributed to high molecular weight charged peptide flux in the pool containing Argireline.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicableElectroosmosis contribution to high-molecular-weight peptide flux
Study
humann: not reported in source
Excised human skin
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
Electroosmosis was an important determinant of the total flux for the high molecular weight charged peptides.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, the permeation of alanine-tryptophan dipeptide (MW 276 Da), alanine-alanine-proline-valine tetrapeptide (MW 355 Da), Argireline® (Acetyl hexapeptide-3, MW 889 Da) and Triptorelin acetate (decapeptide, MW 1311 Da) through excised human skin under passive or iontophoretic current of 0.4…
Summary · context
The effects of pH change (3.0-7.4, to provide different net negative, neutral, and positive charges) to the peptide, donor concentration (1-10 mg/ml), background electrolyte (34-137 mM NaCl and/or 5-20 mM HEPES) and current direction (anodal vs cathodal) were also studied.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-2578687702
03LabNot a test of Argireline (acetyl hexapeptide-8) aloneEx vivo tissuePharmacokinetics
In excised human skin, lower pH decreased permeation of the peptide pool containing Argireline, possibly through decreased electroosmosis.
In plain wordsIn lab tests on human skin, lower pH decreased permeation of the peptide pool containing Argireline.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreasePooled peptide permeation at lower pH
Study
humann: not reported in source
Excised human skin
Comparator
Higher pH
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
Permeation was decreased at lower pH, possibly due to decreased electroosmosis.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, the permeation of alanine-tryptophan dipeptide (MW 276 Da), alanine-alanine-proline-valine tetrapeptide (MW 355 Da), Argireline® (Acetyl hexapeptide-3, MW 889 Da) and Triptorelin acetate (decapeptide, MW 1311 Da) through excised human skin under passive or iontophoretic current of 0.4…
Summary · context
The effects of pH change (3.0-7.4, to provide different net negative, neutral, and positive charges) to the peptide, donor concentration (1-10 mg/ml), background electrolyte (34-137 mM NaCl and/or 5-20 mM HEPES) and current direction (anodal vs cathodal) were also studied.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-2578687703
04LabNot a test of Argireline (acetyl hexapeptide-8) aloneEx vivo tissuePharmacokinetics
In excised human skin, a 10 times donor concentration increase raised permeation of pooled peptides, including Argireline, about 2-4 times.
In plain wordsIn lab tests on human skin, increased donor concentration raised permeation of pooled peptides, including Argireline, about 2-4 times.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
IncreasePooled peptide permeation with increased donor concentration
Study
humann: not reported in source
Excised human skin
Comparator
Lower donor peptide concentration
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
Results also showed that 10 times increase in donor peptide concentration increases permeation of peptides by about 2-4 times and decreases iontophoretic permeability coefficients by about 2.5-5 times.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, the permeation of alanine-tryptophan dipeptide (MW 276 Da), alanine-alanine-proline-valine tetrapeptide (MW 355 Da), Argireline® (Acetyl hexapeptide-3, MW 889 Da) and Triptorelin acetate (decapeptide, MW 1311 Da) through excised human skin under passive or iontophoretic current of 0.4…
Summary · context
The effects of pH change (3.0-7.4, to provide different net negative, neutral, and positive charges) to the peptide, donor concentration (1-10 mg/ml), background electrolyte (34-137 mM NaCl and/or 5-20 mM HEPES) and current direction (anodal vs cathodal) were also studied.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-2578687704
05LabNot a test of Argireline (acetyl hexapeptide-8) aloneEx vivo tissuePharmacokinetics
In excised human skin, a 10 times donor concentration increase reduced pooled peptide iontophoretic permeability coefficients about 2.5-5 times.
In plain wordsIn lab tests on human skin, increased concentration reduced iontophoretic permeability coefficients for pooled peptides, including Argireline, about 2.5-5 times.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreasePooled iontophoretic permeability coefficients with increased concentration
Study
humann: not reported in source
Excised human skin
Comparator
Lower donor peptide concentration
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
Results also showed that 10 times increase in donor peptide concentration increases permeation of peptides by about 2-4 times and decreases iontophoretic permeability coefficients by about 2.5-5 times.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, the permeation of alanine-tryptophan dipeptide (MW 276 Da), alanine-alanine-proline-valine tetrapeptide (MW 355 Da), Argireline® (Acetyl hexapeptide-3, MW 889 Da) and Triptorelin acetate (decapeptide, MW 1311 Da) through excised human skin under passive or iontophoretic current of 0.4…
Summary · context
The effects of pH change (3.0-7.4, to provide different net negative, neutral, and positive charges) to the peptide, donor concentration (1-10 mg/ml), background electrolyte (34-137 mM NaCl and/or 5-20 mM HEPES) and current direction (anodal vs cathodal) were also studied.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-2578687705
06LabNot a test of Argireline (acetyl hexapeptide-8) aloneEx vivo tissuePharmacokinetics
In excised human skin, extra background electrolyte reduced iontophoretic permeation coefficients of pooled peptides, including Argireline, by 2-60 times.
In plain wordsIn lab tests on human skin, extra electrolyte reduced iontophoretic permeation coefficients of pooled peptides, including Argireline, by 2-60 times.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreasePooled iontophoretic permeation coefficient with extra electrolyte
Study
humann: not reported in source
Excised human skin
Comparator
Without extra background electrolyte
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
The addition of extra background electrolyte decreased the iontophoretic permeation coefficient of peptides by 2-60 times.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, the permeation of alanine-tryptophan dipeptide (MW 276 Da), alanine-alanine-proline-valine tetrapeptide (MW 355 Da), Argireline® (Acetyl hexapeptide-3, MW 889 Da) and Triptorelin acetate (decapeptide, MW 1311 Da) through excised human skin under passive or iontophoretic current of 0.4…
Summary · context
The effects of pH change (3.0-7.4, to provide different net negative, neutral, and positive charges) to the peptide, donor concentration (1-10 mg/ml), background electrolyte (34-137 mM NaCl and/or 5-20 mM HEPES) and current direction (anodal vs cathodal) were also studied.
In this study, Argireline (acetyl hexapeptide-8) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Argireline (acetyl hexapeptide-8).
Report a problemFND-2578687706

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
22 Oct 2026, 02:25 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

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