In vitro skin penetration of acetyl hexapeptide-8 from a cosmetic formulation.

Kraeling ME, Zhou W, Wang P, Ogunsola OA

Cutaneous and ocular toxicology22 Apr 2014Cited by 25 (Europe PMC)

PMID24754410DOI10.3109/15569527.2014.894521PepBankPUB-24754410

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissuePharmacokinetics
In excised guinea pig and human skin, most applied acetyl hexapeptide‑8 was washed from the surface.
In plain wordsIn lab tests on guinea pig and human skin, most applied acetyl hexapeptide‑8 remained on the surface.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicablePeptide remaining on skin surface
Study
guinea pig and humann: not reported in source
Hairless guinea pig and human cadaver skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (guinea pig and human).
Source sentences
Summary
The results (percent of applied dose) showed that the majority of the Ac-EEMQRR-amide was washed from the surface of both HGP and human skin.
3 context sentences(design, population, supporting numbers)
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441001
02LabEx vivo tissuePharmacokinetics
In excised guinea pig skin, the penetrated acetyl hexapeptide‑8 remained mostly in the stratum corneum, containing 0.54% of applied dose.
In plain wordsIn lab tests on guinea pig skin, the peptide that penetrated the skin remained mostly in the outer skin layer.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicableStratum corneum retention
Study
guinea pign: not reported in source
Hairless guinea pig skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (guinea pig).
Measurements
Stratum corneum peptide
0.54 % of applied dose
HGP
Source sentences
Summary
Ac-EEMQRR-amide that penetrated skin remained mostly in the stratum corneum of HGP (0.54%) and human (0.22%) with the peptide levels decreasing as each layer was removed by tape stripping.
4 context sentences(design, population, supporting numbers)
Summary · context
The results (percent of applied dose) showed that the majority of the Ac-EEMQRR-amide was washed from the surface of both HGP and human skin.
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441002
03LabEx vivo tissuePharmacokinetics
In human cadaver skin, the penetrated acetyl hexapeptide‑8 remained mostly in the stratum corneum, containing 0.22% of applied dose.
In plain wordsIn lab tests on human skin, the peptide that penetrated the skin remained mostly in the outer skin layer.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicableStratum corneum retention
Study
humann: not reported in source
Human cadaver skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (human).
Measurements
Stratum corneum peptide
0.22 % of applied dose
human
Source sentences
Summary
Ac-EEMQRR-amide that penetrated skin remained mostly in the stratum corneum of HGP (0.54%) and human (0.22%) with the peptide levels decreasing as each layer was removed by tape stripping.
4 context sentences(design, population, supporting numbers)
Summary · context
The results (percent of applied dose) showed that the majority of the Ac-EEMQRR-amide was washed from the surface of both HGP and human skin.
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441003
04LabEx vivo tissuePharmacokinetics
In excised guinea pig and human skin, acetyl hexapeptide‑8 levels decreased with successive stratum corneum tape stripping.
In plain wordsIn lab tests on guinea pig and human skin, acetyl hexapeptide‑8 levels decreased with successive outer skin layers.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
DecreasePeptide levels across stratum corneum layers
Study
guinea pig and humann: not reported in source
Hairless guinea pig and human cadaver skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (guinea pig and human).
Source sentences
Summary
Ac-EEMQRR-amide that penetrated skin remained mostly in the stratum corneum of HGP (0.54%) and human (0.22%) with the peptide levels decreasing as each layer was removed by tape stripping.
3 context sentences(design, population, supporting numbers)
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441004
05LabEx vivo tissuePharmacokinetics
In excised guinea pig skin, epidermal acetyl hexapeptide‑8 recovery was 0.01% of applied dose, similar to human skin.
In plain wordsIn lab tests, guinea pig epidermal acetyl hexapeptide‑8 recovery was 0.01% of applied dose, similar to human skin.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicableEpidermal peptide recovery
Study
guinea pign: not reported in source
Hairless guinea pig skin
Comparator
Human cadaver skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (guinea pig).
Measurements
Epidermal peptide
0.01 % of applied dose
HGP
Source sentences
Summary
Total Ac-EEMQRR-amide found in the epidermis of HGP and human skin was similar at 0.01%.
4 context sentences(design, population, supporting numbers)
Summary · context
The results (percent of applied dose) showed that the majority of the Ac-EEMQRR-amide was washed from the surface of both HGP and human skin.
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441005
06LabEx vivo tissuePharmacokinetics
In human cadaver skin, epidermal acetyl hexapeptide‑8 recovery was 0.01% of applied dose, similar to guinea pig skin.
In plain wordsIn lab tests, human epidermal acetyl hexapeptide‑8 recovery was 0.01% of applied dose, similar to guinea pig skin.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicableEpidermal peptide recovery
Study
humann: not reported in source
Human cadaver skin
Comparator
Hairless guinea pig skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (human).
Measurements
Epidermal peptide
0.01 % of applied dose
human
Source sentences
Summary
Total Ac-EEMQRR-amide found in the epidermis of HGP and human skin was similar at 0.01%.
4 context sentences(design, population, supporting numbers)
Summary · context
The results (percent of applied dose) showed that the majority of the Ac-EEMQRR-amide was washed from the surface of both HGP and human skin.
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441006
07LabEx vivo tissuePharmacokinetics
No acetyl hexapeptide‑8 was detected in the dermis of excised human or guinea pig skin.
In plain wordsIn lab tests, no acetyl hexapeptide‑8 was detected in the dermis of human or guinea pig skin.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicableDermal peptide detection
Study
guinea pig and humann: not reported in source
Hairless guinea pig and human cadaver skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (guinea pig and human).
Source sentences
Summary
No peptide was detected in the dermis or buffer collected underneath the skin for both human and HGP.
3 context sentences(design, population, supporting numbers)
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441007
08LabEx vivo tissuePharmacokinetics
No acetyl hexapeptide‑8 was detected in buffer beneath excised human or guinea pig skin.
In plain wordsIn lab tests, no acetyl hexapeptide‑8 was detected in buffer beneath human or guinea pig skin.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicablePeptide detection in receptor buffer
Study
guinea pig and humann: not reported in source
Hairless guinea pig and human cadaver skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (guinea pig and human).
Source sentences
Summary
No peptide was detected in the dermis or buffer collected underneath the skin for both human and HGP.
3 context sentences(design, population, supporting numbers)
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441008
09LabEx vivo tissuePharmacokinetics
No hexapeptide metabolite was detected in guinea pig or human skin layers or buffer after acetyl hexapeptide‑8 exposure.
In plain wordsIn lab tests, no hexapeptide metabolite was detected in guinea pig or human skin layers or buffer.
Peptide
Argireline (acetyl hexapeptide-8)
Outcome
Not applicableHexapeptide metabolite detection
Study
guinea pig and humann: not reported in source
Hairless guinea pig and human cadaver skin
Outcome group
Pharmacokinetics · Pharmacology
Studied dose
10% at 2 mg/cm(2) · topical · 24 hAs given in this study (guinea pig and human).
Source sentences
Summary
There was no hexapeptide metabolite (H2N-EEMQRR-amide) detected in any layers of HGP skin, human skin or buffer collected underneath the skin.
3 context sentences(design, population, supporting numbers)
Summary · context
Therefore, the skin penetration of commercially available Ac-EEMQRR-amide from a cosmetic formulation (oil-in-water (O/W) emulsion) was determined in hairless guinea pig (HGP) and human cadaver skin assembled into in vitro diffusion cells.
Summary · context
An O/W emulsion containing 10% Ac-EEMQRR-amide was applied to skin at a dose of 2 mg/cm(2).
Summary · context
After a 24-h exposure, the skin surface was washed to remove unabsorbed peptide.
Report a problemFND-2475441009

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
22 Oct 2026, 02:25 UTC
Study results updated
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

Keywords

PepBank is a research reference. Talk to a doctor before you use any peptide.