Peptides / Immune and antimicrobial

Conjugate · 32 amino acids · 4,032 Da

PNC-27

Not approvedBanned in sport

PNC-27 is a research peptide that joins a piece of the p53 protein to a sequence that enters cell membranes. It is not approved and is banned in sport as an unapproved substance, and it was studied only in cells and animals, mostly on cancer. PepBank shows 41 results on PNC-27 from animal and lab studies. No study in people has reported results yet. Data as of 2026-10-30.

Also known as PNC27

32 amino acids, from first to last1 changed amino acid

Evidence level: Animal and lab studies onlyNo study in people has results. ClinicalTrials.gov lists no trial.

Results in people

0% of 41 results

Results in animals

12% of 41 results

Results in lab cells

88% of 41 results

From computer models

0% of 41 results

Overview

PNC-27 at a glance

What it is	Conjugate, 32 amino acids
Approval	Not approved for any use
In sport	Banned in sport (WADA S0)
Studies	20 studies with results. 12 of them gave PNC-27, with 41 results. 5 in animals36 in lab cells
Clinical trials	None registered
Community use	Unproven self-treatment of cancer

The science

What is PNC-27?

A 32-residue research conjugate of a p53 segment and a membrane-penetrating sequence.

Amino acids in one chain

PNC-27 structure and chemistry

The key facts about the molecule. Long codes are in the closed section below.

Sequence, 32 amino acidsFrom the first amino acid to the last

P1PLSQ5−ETFS−D10LW+KLL15+K+KW+KM20+R+RNQF25WV+KVQ30+RG

Darker means more water-repelling+ and − show charge in the bodyOutlined: changed amino acid

Structure

PNC-27 is a conjugate of 32 amino acids, with no ring, 1 changed amino acid.

Formula

C₁₈₈H₂₉₃N₅₃O₄₄S

Molecular weight

4,032 g/mol

Target

MDM2 (HDM-2) (gene MDM2): proposed membrane bindingEvidence: curated database or regulator description

Development

Highest phase: preclinical.

Identifiers

PubChem 16201774, UniProt P04637

Full sequence, SMILES and InChIKey

InChIKey
MZCACYLMMMXSKC-KOCFKOPYSA-N

SMILES
C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC2=CNC3=CC=CC=C32)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC4=CNC5=CC=CC=C54)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC6=CC=CC=C6)C(=O)N[C@@H](CC7=CNC8=CC=CC=C87)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]9CCCN9C(=O)[C@@H]1CCCN1)O

See charge, composition and 3D structure on the Scientific page

The evidence

What PNC-27 studies show

Results grouped by what the studies measured. Each card shows where its results come from.

Animal and lab studies onlyNo study in people has results. ClinicalTrials.gov lists no trial.

Cancer

13 results · 3 animal · 10 lab

In mice with human or mouse leukemia, PNC‑27 killed bulk leukemia blasts in primary transplant experiments. Leukemia, 2020

Cells, genes and aging

1 result · 1 lab

In cells, mitochondria failed to retain the mitochondrial dye after PNC‑27 treatment. Annals of clinical and laboratory science, 2024

Key PNC-27 studies

Studies in people come first, then studies in animals and in the lab.

Animals
Targeting cell membrane HDM2: A novel therapeutic approach for acute myeloid leukemia
Wang et al. 2020 · Leukemia · cited by 14
9 results
Animals
Molecular Targeting of H/MDM-2 Oncoprotein in Human Colon Cancer Cells and Stem-like Colonic Epithelial-derived Progenitor Cells
Thadi et al. 2021 · Anticancer research · cited by 4
7 results
Lab cells
Chondroitin sulfate as a molecular portal that preferentially mediates the apoptotic killing of tumor cells by penetratin-directed mitochondria-disrupting peptides
Yang et al. 2010 · The Journal of biological chemistry · cited by 32
1 result
Lab cells
Anticancer peptide PNC-27 adopts an HDM-2-binding conformation and kills cancer cells by binding to HDM-2 in their membranes
Sarafraz-Yazdi et al. 2010 · Proceedings of the National Academy of Sciences of the United States of America · cited by 23
1 result
Lab cells
The anti-cancer peptide, PNC-27, induces tumor cell lysis as the intact peptide
Sookraj et al. 2010 · Cancer chemotherapy and pharmacology · cited by 15
3 results

All 20 PNC-27 studies

PNC-27 clinical trials

ClinicalTrials.gov lists no trial of PNC-27. A trial under another name may exist.

Status and safety

Is PNC-27 approved?

FDA

United States

Not approved

No approved medicine contains it.

EMA

European Union

Not approved

No approved medicine contains it.

WADA

Sport

Banned

On the 2026 Prohibited List, section S0.

No. Not approved for any use. Preclinical research only. No clinical trial phase found. The FDA, the EMA and the other sources we use show no approval (as of 21 Oct 2026). Banned in sport (WADA S0).

Laws and labels change, so check the regulator for the current status.

PNC-27 side effects and safety

PepBank has 6 safety results from studies of PNC-27. None of them come from studies in people, so they do not show what happens in people.

Safety results from studies (6)

In mice, PNC‑27 spared normal blood stem cell activity in primary and secondary bone marrow transplant experiments. (Leukemia, 2020)
In animals, PNC‑27 did not cause normal tissue necrosis. (Anticancer research, 2021)
In cells from a normal breast epithelial line, cells remained alive during PNC‑27 exposure, unlike cancer cells. (Cancer chemotherapy and pharmacology, 2010)
In cells used as normal lymphocyte controls, PNC‑27 did not kill cells. (Annals of clinical and laboratory science, 2014)
In cells, PNC‑27 showed no toxicity in non-transformed HUVEC. (Annals of clinical and laboratory science, 2020)
In cells, PNC‑27 did not kill normal human cervical cells. (Annals of clinical and laboratory science, 2025)

See every safety result on the Scientific page

From the community

How people use it

What people report online. It comes from forums and guides, not from studies.

Community-reported. Not tested in trials.

A community wiki describes extremely limited PNC-27 use against cancer. It reports no established dose or community protocol.

Reported routines and cautions

What people use it for

Unproven self-treatment of cancerS1

Reported regimens

Regimens that community sources report for PNC-27. Community-reported, not tested in trials.
Context	Route	Amount	How often	How long	Agreement	Sources
Exploratory cancer-use reports	subcutaneous injection	Not stated	Not stated	Not stated	Single source. The source carries seller rankings and seller links.	S1

Cautions

The source states that no human clinical trials have been conducted. This is not a proven cancer treatment.S1
The source warns that self-treatment can delay proven cancer care and describes a report of massive gastrointestinal hemorrhage.S1

Community sources

S1PNC-27 community reports and limitationswww.peptideprotocolwiki.comcarries seller links, not linked

Gaps

The wiki compiles unverified reports. No numeric dose is established.

Questions and more

Ask a question about PNC-27

The answer links to every study it uses.

What does the evidence say about PNC-27?Is PNC-27 tested in people?Compare with Thymosin alpha-1 Compare with LTX-315

Sources

Database: PepBank data for PNC-27 (data version 308560)
Regulator: FDA status record
Regulator: EMA status record
Regulator: WADA status record
Community: community sources (forums and guides), as summarized in the PepBank community file

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

PepBank is a research reference. Talk to a doctor before you use any peptide.

Ask your AI about PNC-27

Curo is a peptide shop. PepBank and Curo have the same owner. See the Curo catalog (opens in a new tab).

PNC-27

Not approvedBanned in sport

Also known as PNC27

What it is

Conjugate, 32 amino acids

Approval

Not approved for any use

In sport

Banned in sport (WADA S0)

Studies

20 studies with results. 12 of them gave PNC-27, with 41 results.
5 in animals36 in lab cells

Clinical trials

None registered

Community use

Unproven self-treatment of cancer

Context

Route

Amount

How often

How long

Agreement

Sources

Exploratory cancer-use reports

subcutaneous injection

Not stated

Single source. The source carries seller rankings and seller links.

PNC-27

PNC-27 at a glance

What is PNC-27?

PNC-27 structure and chemistry