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Study · In vitro (cells) · 2020

4 results

Anti-Cancer Tumor Cell Necrosis of Epithelial Ovarian Cancer Cell Lines Depends on High Expression of HDM-2 Protein in Their Membranes.

Thadi A, Gleeson EM, Khalili M, Shaikh MF, Goldstein E, Morano WF, Daniels LM, Grandhi N, Glatthorn H, Richard SD, Campbell PM, Sarafraz-Yazdi E, Pincus MR, Bowne WB

Annals of clinical and laboratory science1 Sept 2020Cited by 1 (Europe PMC)

PMID33067207 (opens in a new tab)PepBankPUB-33067207
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In ovarian cancer cells, PNC‑27 colocalized with membrane HDM-2.

    In plain wordsIn cells, PNC‑27 associated with membrane HDM-2 in both ovarian cancer cell lines.

    Peptide
    PNC-27
    Outcome
    Not applicableMembrane HDM-2 colocalization
    Study

    humann: not reported in source

    SKOV-3 and OVCAR-3 ovarian cancer cells
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Notably, PNC-27 colocalized with HDM-2 in the membranes of both cancer cell lines that resulted in rapid cellular necrosis.
    2 context sentences
    Summary · context
    Herein, we measured HDM-2 membrane expression in two ovarian cancer cell lines (SKOV-3 and OVCAR-3) and a non-transformed control cell line (HUVEC) by flow cytometric and western blot analysis.
    Summary · context
    Treatment effects with PNC-27 and control peptide were assessed using a MTT cell proliferation assay while direct cytotoxicity was measured by lactate dehydrogenase (LDH) release and induction of apoptotic markers; annexin V and caspase-3.
  2. 02LabIn vitro (cells)Efficacy outcome

    In ovarian cancer cells, PNC‑27 colocalization with membrane HDM-2 resulted in rapid necrosis.

    In plain wordsIn cells, PNC‑27 binding to membrane HDM-2 resulted in rapid ovarian cancer cell death.

    Peptide
    PNC-27
    Outcome
    IncreaseRapid cellular necrosis
    Study

    humann: not reported in source

    SKOV-3 and OVCAR-3 ovarian cancer cells
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Notably, PNC-27 colocalized with HDM-2 in the membranes of both cancer cell lines that resulted in rapid cellular necrosis.
    2 context sentences
    Summary · context
    Herein, we measured HDM-2 membrane expression in two ovarian cancer cell lines (SKOV-3 and OVCAR-3) and a non-transformed control cell line (HUVEC) by flow cytometric and western blot analysis.
    Summary · context
    Treatment effects with PNC-27 and control peptide were assessed using a MTT cell proliferation assay while direct cytotoxicity was measured by lactate dehydrogenase (LDH) release and induction of apoptotic markers; annexin V and caspase-3.
  3. 03LabIn vitro (cells)Mechanism

    In non-transformed HUVEC, no PNC‑27 colocalization was observed.

    In plain wordsIn cells, PNC‑27 did not show colocalization in non-transformed HUVEC.

    Peptide
    PNC-27
    Outcome
    Not applicablePNC-27 colocalization
    Study

    humann: not reported in source

    non-transformed HUVEC
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In contrast, no PNC-27 colocalization and cytotoxicity was observed with non-transformed HUVEC demonstrating minimal expression of membrane HDM-2.
    2 context sentences
    Summary · context
    Herein, we measured HDM-2 membrane expression in two ovarian cancer cell lines (SKOV-3 and OVCAR-3) and a non-transformed control cell line (HUVEC) by flow cytometric and western blot analysis.
    Summary · context
    Treatment effects with PNC-27 and control peptide were assessed using a MTT cell proliferation assay while direct cytotoxicity was measured by lactate dehydrogenase (LDH) release and induction of apoptotic markers; annexin V and caspase-3.
  4. 04LabIn vitro (cells)Safety outcome

    In non-transformed HUVEC, no PNC‑27 cytotoxicity was observed.

    In plain wordsIn cells, PNC‑27 showed no toxicity in non-transformed HUVEC.

    Peptide
    PNC-27
    Outcome
    Not applicableCytotoxicity
    Study

    humann: not reported in source

    non-transformed HUVEC
    Outcome group
    Adverse events · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In contrast, no PNC-27 colocalization and cytotoxicity was observed with non-transformed HUVEC demonstrating minimal expression of membrane HDM-2.
    2 context sentences
    Summary · context
    Herein, we measured HDM-2 membrane expression in two ovarian cancer cell lines (SKOV-3 and OVCAR-3) and a non-transformed control cell line (HUVEC) by flow cytometric and western blot analysis.
    Summary · context
    Treatment effects with PNC-27 and control peptide were assessed using a MTT cell proliferation assay while direct cytotoxicity was measured by lactate dehydrogenase (LDH) release and induction of apoptotic markers; annexin V and caspase-3.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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