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Study · In vitro (cells) · 2025

4 results

HDM-2-Targeting Peptide PNC-27 Kills Cervical Cancer Cells but not Normal Cervical Cells.

Krzesaj PK, Seydafkan S, Miller AI, Chen HT, Premsrirut P, Shim A, Mcglinchey S, Yazdi E, Brandt-Rauf P, Silberstein M, Jahari G, Feinman RD, Pincus MR

Annals of clinical and laboratory science1 May 2025

PMID40750238 (opens in a new tab)PepBankPUB-40750238
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In human HTB-35 cervical cancer cells, PNC‑27 was cytotoxic with an IC50 of 12.4 μM.

    In plain wordsIn cells, PNC‑27 killed human cervical cancer cells.

    Peptide
    PNC-27
    Outcome
    IncreaseCervical cancer cell cytotoxicity
    Study

    humann: not reported in source

    HTB-35 and PCS-480 cervical cells
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Measurements

    IC50
    12.4 μM
    HTB-35 cervical cancer cells

    Source sentences

    Summary
    We found that PNC-27 is cytotoxic even at low doses (IC50=12.4 μM) to the human HTB-35 cervical cancer squamous epithelial cell line but not to a counterpart normal human PCS-480 cell line.
    1 context sentence
    Summary · context
    We determined dose response curves for incubation of PNC-27 with the human squamous cervical cancer cell line HTB-35 (also called SiHa cells) and with the untransformed human squamous cervical cell line, PCS-480.
  2. 02LabIn vitro (cells)Safety outcome

    In normal human PCS-480 cervical cells, PNC‑27 was not cytotoxic.

    In plain wordsIn cells, PNC‑27 did not kill normal human cervical cells.

    Peptide
    PNC-27
    Outcome
    No significant changeNormal cervical cell cytotoxicity
    Study

    humann: not reported in source

    HTB-35 and PCS-480 cervical cells
    Outcome group
    Adverse events · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We found that PNC-27 is cytotoxic even at low doses (IC50=12.4 μM) to the human HTB-35 cervical cancer squamous epithelial cell line but not to a counterpart normal human PCS-480 cell line.
    1 context sentence
    Summary · context
    We determined dose response curves for incubation of PNC-27 with the human squamous cervical cancer cell line HTB-35 (also called SiHa cells) and with the untransformed human squamous cervical cell line, PCS-480.

Studies that did not test the peptide alone2

In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking PNC-27.

  1. 03LabNot a test of PNC-27 aloneIn vitro (cells)Mechanism

    In cell culture and alcoholic preservatives, HTB-35 cancer cells expressed high membrane HDM-2 levels, whereas normal PCS-480 cells did not.

    In plain wordsIn cells, the proposed PNC‑27 target HDM-2 appeared on cervical cancer cell membranes but not normal cervical cell membranes.

    Peptide
    PNC-27
    Outcome
    IncreaseMembrane HDM-2 expression
    Study

    humann: not reported in source

    HTB-35 and PCS-480 cervical cells
    Comparator
    Normal PCS-480 cells
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    We found that HTB-35 cells express high levels of HDM-2 proteins in their membranes both in cell culture and in alcoholic preservative solutions but that the normal PCS-480 cells do not.
    1 context sentence
    Summary · context
    We determined dose response curves for incubation of PNC-27 with the human squamous cervical cancer cell line HTB-35 (also called SiHa cells) and with the untransformed human squamous cervical cell line, PCS-480.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

  2. 04LabNot a test of PNC-27 aloneIn vitro (cells)Mechanism

    In HTB-35 cervical cancer cells, membrane HDM-2 expression remained detectable in cell culture and alcoholic preservative solutions.

    In plain wordsIn lab tests, cervical cancer cells retained detectable membrane HDM-2 in culture and alcoholic preservative solutions.

    Peptide
    PNC-27
    Outcome
    Not applicableMembrane HDM-2 preservation stability
    Study

    humann: not reported in source

    HTB-35 cervical cancer cells in culture and alcoholic preservative solutions
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    We found that HTB-35 cells express high levels of HDM-2 proteins in their membranes both in cell culture and in alcoholic preservative solutions but that the normal PCS-480 cells do not.
    1 context sentence
    Summary · context
    Our results further suggest that the expression of membrane-bound HDM-2 on cervical cancer cells is stable both in cell culture media and in alcoholic preservative fluid.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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