Skip to content

Study · In vitro (cells) · 2010

3 results

Anticancer peptide PNC-27 adopts an HDM-2-binding conformation and kills cancer cells by binding to HDM-2 in their membranes.

Sarafraz-Yazdi E, Bowne WB, Adler V, Sookraj KA, Wu V, Shteyler V, Patel H, Oxbury W, Brandt-Rauf P, Zenilman ME, Michl J, Pincus MR

Proceedings of the National Academy of Sciences of the United States of America11 Jan 2010Cited by 23 (Europe PMC)

PMID20080680 (opens in a new tab)PMCIDPMC2836618 (opens in a new tab)DOI10.1073/pnas.0909364107 (opens in a new tab)PepBankPUB-20080680
Source linkPubMedAsk about this study
Jump to 3 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In cancer cell colocalization experiments, PNC‑27 bound membrane-associated HDM-2.

    In plain wordsIn cells, PNC‑27 bound HDM-2 in cell membranes.

    Peptide
    PNC-27
    Outcome
    Not applicableMembrane HDM-2 binding
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In colocalization experiments, we find that PNC-27 binds to cell membrane-bound HDM-2.

Studies that did not test the peptide alone2

In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking PNC-27.

  1. 02LabNot a test of PNC-27 aloneIn vitro (cells)Mechanism

    In MCF-10-2A cells, expression of membrane-targeted full-length HDM-2 conferred susceptibility to PNC‑27.

    In plain wordsIn cells, engineered membrane HDM-2 made previously resistant MCF-10-2A cells susceptible to PNC‑27.

    Peptide
    PNC-27
    Outcome
    IncreasePNC-27 susceptibility after membrane HDM-2 expression
    Study

    humann: not reported in source

    Comparator
    Untransformed MCF-10-2A cells without the membrane-targeted HDM-2 construct
    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    We further transfected a plasmid expressing full-length HDM-2 with a membrane-localization signal into untransformed MCF-10-2A cells not susceptible to PNC-27 and found that these cells expressing full-length HDM-2 on their cell surface became susceptible to PNC-27.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

  2. 03LabNot a test of PNC-27 aloneIn vitro (cell-free)Mechanism

    In structural comparisons, the p53 region of PNC‑27 was directly superimposable on the corresponding HDM-2-bound residues.

    In plain wordsIn lab tests, the p53 portion of PNC‑27 matched the structure of the same residues bound to HDM-2.

    Peptide
    PNC-27
    Outcome
    Not applicableHDM-2-binding structural alignment
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Cancer

    Source sentences

    Summary
    We find that our previously determined 3D structure of the p53 residues of PNC-27 is directly superimposable on the structure for the same residues bound to HDM-2, suggesting that the peptide may target HDM-2 in the membranes of cancer cells.

    In this study, PNC-27 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking PNC-27.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.