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Study · Animal study (in vivo) · 2021

7 results

Molecular Targeting of H/MDM-2 Oncoprotein in Human Colon Cancer Cells and Stem-like Colonic Epithelial-derived Progenitor Cells.

Thadi A, Morano WF, Khalili M, Babcock BD, Shaikh MF, Foster DS, Piazza Y, Gleeson EM, Goldstein E, Steele L, Campbell PM, Lin BO, Pincus MR, Bowne WB

Anticancer research1 Jan 2021Cited by 4 (Europe PMC)

PMID33419797 (opens in a new tab)DOI10.21873/anticanres.14749 (opens in a new tab)PepBankPUB-33419797
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In an in vivo colon cancer model, PNC‑27 caused tumor nodule necrosis.

    In plain wordsIn animals, PNC‑27 caused tumor nodule necrosis.

    Peptide
    PNC-27
    Outcome
    IncreaseTumor nodule necrosis
    Study

    n: not reported in source

    colon tumor nodules
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vivo, PNC-27 caused necrosis of tumor nodules but not of normal tissue.
  2. 02AnimalAnimal study (in vivo)Safety outcome

    In an in vivo colon cancer model, PNC‑27 did not cause normal tissue necrosis.

    In plain wordsIn animals, PNC‑27 did not cause normal tissue necrosis.

    Peptide
    PNC-27
    Outcome
    Not applicableNormal tissue necrosis
    Study

    n: not reported in source

    colon cancer model
    Outcome group
    Adverse events · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vivo, PNC-27 caused necrosis of tumor nodules but not of normal tissue.
  3. 03LabIn vitro (cells)Mechanism

    In colon cell lines, PNC‑27 colocalized with membrane HDM-2 only in cancer cells.

    In plain wordsIn cells, PNC‑27 associated with membrane HDM-2 only in cancer cells.

    Peptide
    PNC-27
    Outcome
    Not applicableMembrane HDM-2 colocalization
    Study

    humann: not reported in source

    colon cancer cell lines
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    PNC-27 co-localized with membrane HDM-2 only in the cancer cells and caused total cell death (tumor cell necrosis, high LDH release, negative annexin V and caspase 3).
    2 context sentences
    Summary · context
    Flow cytometry determined the CD44 and HDM-2 expression on six-colon cancer cell lines and one normal cell line (CCD-18Co).
    Summary · context
    MTT, LDH release, annexin V binding and caspase 3 assays were used to assess PNC-27-induced cell death.
  4. 04LabIn vitro (cells)Efficacy outcome

    In colon cancer cell lines, PNC‑27 caused total cell death through necrosis.

    In plain wordsIn cells, PNC‑27 killed all colon cancer cells through necrosis.

    Peptide
    PNC-27
    Outcome
    IncreaseCancer cell death
    Study

    humann: not reported in source

    colon cancer cell lines
    Outcome group
    Tumor growth · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    PNC-27 co-localized with membrane HDM-2 only in the cancer cells and caused total cell death (tumor cell necrosis, high LDH release, negative annexin V and caspase 3).
    2 context sentences
    Summary · context
    Flow cytometry determined the CD44 and HDM-2 expression on six-colon cancer cell lines and one normal cell line (CCD-18Co).
    Summary · context
    MTT, LDH release, annexin V binding and caspase 3 assays were used to assess PNC-27-induced cell death.
  5. 05LabIn vitro (cells)Mechanism

    In colon cancer cells, PNC‑27-induced necrosis involved high LDH release.

    In plain wordsIn cells, PNC‑27 treatment produced high LDH release.

    Peptide
    PNC-27
    Outcome
    IncreaseLDH release
    Study

    humann: not reported in source

    colon cancer cell lines
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    PNC-27 co-localized with membrane HDM-2 only in the cancer cells and caused total cell death (tumor cell necrosis, high LDH release, negative annexin V and caspase 3).
    2 context sentences
    Summary · context
    Flow cytometry determined the CD44 and HDM-2 expression on six-colon cancer cell lines and one normal cell line (CCD-18Co).
    Summary · context
    MTT, LDH release, annexin V binding and caspase 3 assays were used to assess PNC-27-induced cell death.
  6. 06LabIn vitro (cells)Mechanism

    In colon cancer cells, PNC‑27-induced death showed negative annexin V results.

    In plain wordsIn cells, annexin V results were negative during PNC‑27-induced cancer cell death.

    Peptide
    PNC-27
    Outcome
    Not applicableAnnexin V
    Study

    humann: not reported in source

    colon cancer cell lines
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    PNC-27 co-localized with membrane HDM-2 only in the cancer cells and caused total cell death (tumor cell necrosis, high LDH release, negative annexin V and caspase 3).
    2 context sentences
    Summary · context
    Flow cytometry determined the CD44 and HDM-2 expression on six-colon cancer cell lines and one normal cell line (CCD-18Co).
    Summary · context
    MTT, LDH release, annexin V binding and caspase 3 assays were used to assess PNC-27-induced cell death.
  7. 07LabIn vitro (cells)Mechanism

    In colon cancer cells, PNC‑27-induced death showed negative caspase 3 results.

    In plain wordsIn cells, caspase 3 results were negative during PNC‑27-induced cancer cell death.

    Peptide
    PNC-27
    Outcome
    Not applicableCaspase 3
    Study

    humann: not reported in source

    colon cancer cell lines
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    PNC-27 co-localized with membrane HDM-2 only in the cancer cells and caused total cell death (tumor cell necrosis, high LDH release, negative annexin V and caspase 3).
    2 context sentences
    Summary · context
    Flow cytometry determined the CD44 and HDM-2 expression on six-colon cancer cell lines and one normal cell line (CCD-18Co).
    Summary · context
    MTT, LDH release, annexin V binding and caspase 3 assays were used to assess PNC-27-induced cell death.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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