- Average mass
- 1,799.10 Da
- Sum of ExPASy residue masses + water + 1 disulfide · ExPASy Compute pI/Mw; FindMod
- Monoisotopic mass
- 1,797.8654 Da
- Sum of ExPASy residue masses + water + 1 disulfide · ExPASy FindMod
- Isoelectric point (pI)
- 10.40
- pH of zero net charge, EMBOSS pKa set · EMBOSS iep, Epk.dat
- Net charge at pH 7.4
- 0.94
- Henderson-Hasselbalch, EMBOSS pKa set · EMBOSS iep, Epk.dat
- GRAVY (hydropathy)
- 0.556
- Mean Kyte-Doolittle hydropathy · Kyte and Doolittle 1982
- Positive: hydrophobic on average.
- Extinction coefficient, 280 nm (Cys reduced)
- 0 M⁻¹ cm⁻¹
- 5500 x Trp + 1490 x Tyr · Pace et al. 1995 (ExPASy ProtParam)
- No Trp or Tyr: absorbance at 280 nm cannot measure this peptide.
- Extinction coefficient, 280 nm (all Cys as cystines)
- 125 M⁻¹ cm⁻¹
- 5500 x Trp + 1490 x Tyr + 125 x cystine · Pace et al. 1995 (ExPASy ProtParam)
- Instability index
- 59.23
- Dipeptide instability weights (DIWV) · Guruprasad et al. 1990
- Above 40: classed as possibly unstable (ExPASy).
- Aliphatic index
- 85.0
- Mole % A + 2.9 V + 3.9 (I + L) · Ikai 1980
- Boman index
- 1.29 kcal/mol
- Mean solubility value (negated) · Boman 2003
- 2.48 or below: lower protein-binding potential (Boman).
- Hydrophobic moment (μH)
- 0.246
- Eisenberg scale, 100° per residue, best 11-residue window · Eisenberg et al. 1982, 1984
- pI with each pKa set
- EMBOSS 10.40Bjellqvist (ExPASy) 9.60IPC peptide 10.88IPC protein 10.31