Simplifying and expanding the screening for peptides <2 kDa by direct urine injection, liquid chromatography, and ion mobility mass spectrometry.

Thomas A, Görgens C, Guddat S, Thieme D, Dellanna F, Schänzer W, Thevis M

Journal of separation science15 Dec 2015Cited by 38 (Europe PMC)

PMID26578461DOI10.1002/jssc.201501060PepBankPUB-26578461

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study22

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone22

In this study, the peptide was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking the peptide.

01HumanNot a test of GHRP-6 aloneOther designOther
Proof of concept was shown by analysing elimination study urine samples collected from individuals who had administered GHRP‑6, GHRP-2 or LHRH.
In plain wordsThe urine method detected peptides in samples from people who had used GHRP‑6, GHRP-2 or LHRH.
Peptide
GHRP-6
Outcome
Not applicableDetection in elimination study urine after GHRP-6 administration
Study
n: not reported in source
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
Finally, proof-of-concept was shown by analysing elimination study urine samples collected from individuals having administered GHRP-6, GHRP-2, or LHRH.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-2657846115
02LabNot a test of GHRP-6 aloneIn vitro (cell-free)Other
A direct urine injection LC ion-mobility mass spectrometry method for a panel of peptides including GHRP‑6 reached limits of detection of 50 to 500 pg/mL.
In plain wordsIn lab tests, a urine test method for a panel of banned peptides, GHRP‑6 among them, detected very small amounts.
Peptide
GHRP-6
Outcome
Not applicableLimits of detection for peptide panel
Study
n: not reported in source
Urine, direct injection LC ion mobility MS
Outcome group
Pharmacokinetics · Pharmacology
Measurements
Limit of detection
50 to 500 pg/mL
peptide panel
Source sentences
Summary
The accomplished sensitivity allows for limits of detection between 50 and 500 pg/mL and thus covers the minimum required performance level of 2 ng/mL accordingly.
1 context sentence(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-2657846113
03LabNot a test of GHRP-6 aloneIn vitro (cell-free)Other
The stability of the panel peptides in urine was tested, and -20 degrees C was the appropriate storage temperature.
In plain wordsIn lab tests, urine samples with these peptides, GHRP‑6 among them, kept well when stored at -20 degrees.
Peptide
GHRP-6
Outcome
Not applicableStability of peptides in urine
Study
n: not reported in source
Urine storage stability
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
The stability of the peptides in urine was tested, and -20°C was found to be the appropriate storage temperature for sports drug testing.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-2657846114
04LabNot a test of AOD-9604 aloneOther designOther
Direct urine injection with LC-IM-MS reached limits of detection between 50 and 500 pg/mL for the panel that includes AOD‑9604.
In plain wordsIn lab tests, a urine test could detect AOD‑9604 at very low levels.
Peptide
AOD-9604
Outcome
Not applicableLimit of detection in urine
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Limit of detection
50 to 500 pg/mL
urine, LC-IM-MS
Source sentences
Summary
limits of detection between 50 and 500 pg/mL
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, AOD-9604 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AOD-9604.
Report a problemFND-2657846101
05LabNot a test of AOD-9604 aloneOther designOther
The urine method for the panel that includes AOD‑9604 was precise (imprecision below 20%) and linear between 0 and 10 ng/mL.
In plain wordsIn lab tests, the urine test for AOD‑9604 gave consistent results over a set range.
Peptide
AOD-9604
Outcome
Not applicablePrecision and linearity
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Imprecision
< 20%
urine
Linear range
0 to 10 ng/mL
urine
Source sentences
Summary
The method is precise (imprecision <20%) and linear in the estimated working range between 0 and 10 ng/mL.
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, AOD-9604 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AOD-9604.
Report a problemFND-2657846102
06LabNot a test of AOD-9604 aloneOther designOther
In urine, the panel that includes AOD‑9604 was stable at -20°C, which was the appropriate storage temperature.
In plain wordsIn lab tests, AOD‑9604 in urine kept well when stored frozen at -20°C.
Peptide
AOD-9604
Outcome
Not applicableStability in urine
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
The stability of the peptides in urine was tested, and -20°C was found to be the appropriate storage temperature for sports drug testing.
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, AOD-9604 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AOD-9604.
Report a problemFND-2657846103
07LabNot a test of Cibinetide (ARA-290) aloneOther designOther
Direct urine injection with LC-IM-MS reached limits of detection between 50 and 500 pg/mL for the panel that includes ARA‑290.
In plain wordsIn lab tests, a urine test could detect ARA‑290 at very low levels.
Peptide
Cibinetide (ARA-290)
Outcome
Not applicableLimit of detection in urine
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Limit of detection
50 to 500 pg/mL
urine, LC-IM-MS
Source sentences
Summary
limits of detection between 50 and 500 pg/mL
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, Cibinetide (ARA-290) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cibinetide (ARA-290).
Report a problemFND-2657846104
08LabNot a test of Cibinetide (ARA-290) aloneOther designOther
The urine method for the panel that includes ARA‑290 was precise (imprecision below 20%) and linear between 0 and 10 ng/mL.
In plain wordsIn lab tests, the urine test for ARA‑290 gave consistent results over a set range.
Peptide
Cibinetide (ARA-290)
Outcome
Not applicablePrecision and linearity
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Imprecision
< 20%
urine
Linear range
0 to 10 ng/mL
urine
Source sentences
Summary
The method is precise (imprecision <20%) and linear in the estimated working range between 0 and 10 ng/mL.
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, Cibinetide (ARA-290) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cibinetide (ARA-290).
Report a problemFND-2657846105
09LabNot a test of Cibinetide (ARA-290) aloneOther designOther
In urine, the panel that includes ARA‑290 was stable at -20°C, which was the appropriate storage temperature.
In plain wordsIn lab tests, ARA‑290 in urine kept well when stored frozen at -20°C.
Peptide
Cibinetide (ARA-290)
Outcome
Not applicableStability in urine
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
The stability of the peptides in urine was tested, and -20°C was found to be the appropriate storage temperature for sports drug testing.
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, Cibinetide (ARA-290) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cibinetide (ARA-290).
Report a problemFND-2657846106
10LabNot a test of Ipamorelin aloneOther designOther
Direct urine injection with LC-IM-MS reached limits of detection between 50 and 500 pg/mL for the panel that includes ipamorelin.
In plain wordsIn lab tests, a urine test could detect ipamorelin at very low levels.
Peptide
Ipamorelin
Outcome
Not applicableLimit of detection in urine
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Limit of detection
50 to 500 pg/mL
urine, LC-IM-MS
Source sentences
Summary
limits of detection between 50 and 500 pg/mL
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, Ipamorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Ipamorelin.
Report a problemFND-2657846107
11LabNot a test of Ipamorelin aloneOther designOther
The urine method for the panel that includes ipamorelin was precise (imprecision below 20%) and linear between 0 and 10 ng/mL.
In plain wordsIn lab tests, the urine test for ipamorelin gave consistent results over a set range.
Peptide
Ipamorelin
Outcome
Not applicablePrecision and linearity
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Imprecision
< 20%
urine
Linear range
0 to 10 ng/mL
urine
Source sentences
Summary
The method is precise (imprecision <20%) and linear in the estimated working range between 0 and 10 ng/mL.
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, Ipamorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Ipamorelin.
Report a problemFND-2657846108
12LabNot a test of Ipamorelin aloneOther designOther
In urine, the panel that includes ipamorelin was stable at -20°C, which was the appropriate storage temperature.
In plain wordsIn lab tests, ipamorelin in urine kept well when stored frozen at -20°C.
Peptide
Ipamorelin
Outcome
Not applicableStability in urine
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
The stability of the peptides in urine was tested, and -20°C was found to be the appropriate storage temperature for sports drug testing.
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, Ipamorelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Ipamorelin.
Report a problemFND-2657846109
13LabNot a test of TB-500 aloneOther designOther
Direct urine injection with LC-IM-MS reached limits of detection between 50 and 500 pg/mL for the panel that includes TB‑500.
In plain wordsIn lab tests, a urine test could detect TB‑500 at very low levels.
Peptide
TB-500
Outcome
Not applicableLimit of detection in urine
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Limit of detection
50 to 500 pg/mL
urine, LC-IM-MS
Source sentences
Summary
limits of detection between 50 and 500 pg/mL
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, TB-500 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking TB-500.
Report a problemFND-2657846110
14LabNot a test of TB-500 aloneOther designOther
The urine method for the panel that includes TB‑500 was precise (imprecision below 20%) and linear between 0 and 10 ng/mL.
In plain wordsIn lab tests, the urine test for TB‑500 gave consistent results over a set range.
Peptide
TB-500
Outcome
Not applicablePrecision and linearity
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Imprecision
< 20%
urine
Linear range
0 to 10 ng/mL
urine
Source sentences
Summary
The method is precise (imprecision <20%) and linear in the estimated working range between 0 and 10 ng/mL.
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, TB-500 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking TB-500.
Report a problemFND-2657846111
15LabNot a test of TB-500 aloneOther designOther
In urine, the panel that includes TB‑500 was stable at -20°C, which was the appropriate storage temperature.
In plain wordsIn lab tests, TB‑500 in urine kept well when stored frozen at -20°C.
Peptide
TB-500
Outcome
Not applicableStability in urine
Study
n: not reported in source
urine
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
The stability of the peptides in urine was tested, and -20°C was found to be the appropriate storage temperature for sports drug testing.
2 context sentences(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
Summary · context
With the presented method employing direct urine injection into a liquid chromatograph followed by ion-mobility time-of-flight mass spectrometry, a facile, specific and sensitive assay for the aforementioned peptidic compounds is provided.
In this study, TB-500 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking TB-500.
Report a problemFND-2657846112
16LabNot a test of GHRP-2 aloneOther designOther
A direct-urine LC-ion mobility MS method for peptides under 2 kDa, including GHRP‑2, had limits of detection between 50 and 500 pg/mL.
In plain wordsIn lab tests, a urine screening method could detect GHRP‑2 and similar peptides at very low levels.
Peptide
GHRP-2
Outcome
Not applicableLimits of detection of urine screening method
Study
n: not reported in source
direct urine injection LC-ion mobility MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Limit of detection
50 to 500 pg/mL
peptides <2 kDa
Source sentences
Summary
The accomplished sensitivity allows for limits of detection between 50 and 500 pg/mL and thus covers the minimum required performance level of 2 ng/mL accordingly.
1 context sentence(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-2657846116
17LabNot a test of Hexarelin aloneOther designOther
A direct-urine LC-ion mobility MS method for peptides under 2 kDa, including hexarelin, had limits of detection between 50 and 500 pg/mL.
In plain wordsIn lab tests, a urine screening method could detect hexarelin and similar peptides at very low levels.
Peptide
Hexarelin
Outcome
Not applicableLimits of detection of urine screening method
Study
n: not reported in source
direct urine injection LC-ion mobility MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Limit of detection
50 to 500 pg/mL
peptides <2 kDa
Source sentences
Summary
The accomplished sensitivity allows for limits of detection between 50 and 500 pg/mL and thus covers the minimum required performance level of 2 ng/mL accordingly.
1 context sentence(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.
Report a problemFND-2657846117
18LabNot a test of GHRP-2 aloneOther designOther
The urine screening method that covers GHRP‑2 was precise (imprecision <20%) and linear between 0 and 10 ng/mL.
In plain wordsIn lab tests, the urine screening method that covers GHRP‑2 gave consistent results across a working range.
Peptide
GHRP-2
Outcome
Not applicablePrecision and linearity of urine screening method
Study
n: not reported in source
direct urine injection LC-ion mobility MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Imprecision
< 20%
method
Linear range
0 to 10 ng/mL
method
Source sentences
Summary
The method is precise (imprecision <20%) and linear in the estimated working range between 0 and 10 ng/mL.
1 context sentence(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-2657846118
19LabNot a test of Hexarelin aloneOther designOther
The urine screening method that covers hexarelin was precise (imprecision <20%) and linear between 0 and 10 ng/mL.
In plain wordsIn lab tests, the urine screening method that covers hexarelin gave consistent results across a working range.
Peptide
Hexarelin
Outcome
Not applicablePrecision and linearity of urine screening method
Study
n: not reported in source
direct urine injection LC-ion mobility MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Imprecision
< 20%
method
Linear range
0 to 10 ng/mL
method
Source sentences
Summary
The method is precise (imprecision <20%) and linear in the estimated working range between 0 and 10 ng/mL.
1 context sentence(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.
Report a problemFND-2657846119
20LabNot a test of GHRP-2 aloneOther designOther
In stability tests of the peptides in urine, -20°C was the appropriate storage temperature for sports drug testing.
In plain wordsIn lab tests, urine samples with GHRP‑2 and similar peptides were stable when stored frozen at -20°C.
Peptide
GHRP-2
Outcome
Not applicableStability of peptides in urine
Study
n: not reported in source
direct urine injection LC-ion mobility MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Storage temperature
−20°C
urine
Source sentences
Summary
The stability of the peptides in urine was tested, and -20°C was found to be the appropriate storage temperature for sports drug testing.
1 context sentence(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-2657846120
21LabNot a test of Hexarelin aloneOther designOther
In stability tests of the peptides in urine, -20°C was the appropriate storage temperature for sports drug testing.
In plain wordsIn lab tests, urine samples with hexarelin and similar peptides were stable when stored frozen at -20°C.
Peptide
Hexarelin
Outcome
Not applicableStability of peptides in urine
Study
n: not reported in source
direct urine injection LC-ion mobility MS assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Storage temperature
−20°C
urine
Source sentences
Summary
The stability of the peptides in urine was tested, and -20°C was found to be the appropriate storage temperature for sports drug testing.
1 context sentence(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.
Report a problemFND-2657846121
22LabNot a test of GHRP-2 aloneOther designOther
The method was applied as proof of concept to elimination study urine samples from individuals who had administered GHRP‑2.
In plain wordsIn lab tests, the urine screening method was tried as a proof of concept on elimination study samples after GHRP‑2 use.
Peptide
GHRP-2
Outcome
Not applicableProof of concept in elimination study urine
Study
humann: not reported in source
elimination study urine samples
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
Finally, proof-of-concept was shown by analysing elimination study urine samples collected from individuals having administered GHRP-6, GHRP-2, or LHRH.
1 context sentence(design, population, supporting numbers)
Summary · context
Several peptides <2 kDa with performance-enhancing properties are covered by the list of prohibited substances of the World Anti-Doping Agency including Desmopressin, LH-RH, Buserelin, Triptorelin, Leuprolide, GHRP-1, GHRP-2, GHRP-3, GHRP-4, GHRP-5,GHRP-6, Alexamorelin, Ipamorelin, Hexarelin, ARA-29…
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-2657846122

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Versions 43 to 51Current
28 Oct 2026, 02:02 UTC
The same notice was added 9 times, first on 22 Oct 2026, 02:25 UTC. The status did not change between them.
Study results updated
Version 42
21 Oct 2026, 18:59 UTC
Study data updated · source update from Europe PMC
Versions 27 to 41
21 Oct 2026, 17:05 UTC
The same notice was added 15 times, first on 13 Oct 2026, 21:43 UTC. The status did not change between them.
Study results updated
Versions 25 to 26
13 Oct 2026, 16:31 UTC
The same notice was added 2 times, first on 13 Oct 2026, 16:31 UTC. The status did not change between them.
Study data updated · source update from Europe PMC
Versions 7 to 24
13 Oct 2026, 14:22 UTC
The same notice was added 18 times, first on 9 Oct 2026, 18:17 UTC. The status did not change between them.
Study results updated
Versions 2 to 6
9 Oct 2026, 03:13 UTC
The same notice was added 5 times, first on 9 Oct 2026, 03:02 UTC. The status did not change between them.
Study data updated · source update from Europe PMC
Version 1
9 Oct 2026, 02:47 UTC
First added · source update from Europe PMC