Effects of BPC-157 and TB-500 on Achilles tendon healing in rats: A histopathological and biomechanical study.

Biçer O, Adanir O, Güleryüz Y, Balci EC, Dinçel YM, Yenigün MY, Aydin C, Bayrak BY

Joint diseases and related surgery23 Jul 2026

PMID42542926DOI10.52312/jdrs.2026.2951PepBankPUB-42542926

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC. Results still quote short attributed sentences.

Results from this study17

11 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, TB‑500 increased maximum load to failure compared with controls (p < 0.05).
In plain wordsIn rats with repaired Achilles tendons, TB‑500 made the tendon stronger than in untreated controls.
Peptide
TB-500
Outcome
IncreaseMaximum load to failure
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
60 µg/kg/day · intraperitoneal · 4 weeksAs given in this study (rat).
Source sentences
Summary
Biomechanical testing revealed higher maximum load to failure values in the BPC-157 and TB-500 groups compared to controls, reaching statistical significance in the TB-500 group (p < 0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
Report a problemFND-4254292601
02AnimalAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, TB‑500 lowered the total Bonar histopathology score compared with controls (p = 0.016).
In plain wordsIn rats with repaired Achilles tendons, TB‑500 gave better tendon tissue scores than no treatment.
Peptide
TB-500
Outcome
DecreaseTotal Bonar score
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
60 µg/kg/day · intraperitoneal · 4 weeksAs given in this study (rat).
Source sentences
Summary
Histopathological evaluation demonstrated significantly lower total Bonar scores in the TB-500 group (p = 0.016)
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
Report a problemFND-4254292602
03AnimalAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, TB‑500 lowered the total Movin histopathology score compared with controls (p = 0.017).
In plain wordsIn rats with repaired Achilles tendons, TB‑500 gave better tendon tissue scores than no treatment.
Peptide
TB-500
Outcome
DecreaseTotal Movin score
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
60 µg/kg/day · intraperitoneal · 4 weeksAs given in this study (rat).
Source sentences
Summary
significantly lower total Movin scores in the TB-500 and BPC + TB groups (p = 0.017 and p = 0.040, respectively)
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
Report a problemFND-4254292603
04AnimalAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, Sirius red birefringence showed increased type I collagen organization in treatment groups, particularly with TB‑500.
In plain wordsIn rats with repaired Achilles tendons, treated tendons, mostly with TB‑500, showed more organized type I collagen.
Peptide
TB-500
Outcome
IncreaseType I collagen organization (Sirius red)
Study
ratn: not reported in source
Achilles tendon transection and repair
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
60 µg/kg/day · intraperitoneal · 4 weeksAs given in this study (rat).
Source sentences
Summary
Sirius red birefringence analysis showed increased type I collagen organization and altered type III collagen distribution in treatment groups, particularly in the TB-500 group, suggesting progression toward matrix maturation.
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
Report a problemFND-4254292605
05AnimalAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, Sirius red birefringence showed altered type III collagen distribution in treatment groups, particularly with TB‑500.
In plain wordsIn rats with repaired Achilles tendons, treated tendons, mostly with TB‑500, showed a changed spread of type III collagen.
Peptide
TB-500
Outcome
Direction not reportedType III collagen distribution (Sirius red)
Study
ratn: not reported in source
Achilles tendon transection and repair
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
60 µg/kg/day · intraperitoneal · 4 weeksAs given in this study (rat).
Source sentences
Summary
Sirius red birefringence analysis showed increased type I collagen organization and altered type III collagen distribution in treatment groups, particularly in the TB-500 group, suggesting progression toward matrix maturation.
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
Report a problemFND-4254292606
06AnimalAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, collagen type I expression did not differ significantly among groups.
In plain wordsIn rats with repaired Achilles tendons, TB‑500 did not change the amount of type I collagen compared with other groups.
Peptide
TB-500
Outcome
No significant changeCollagen type I expression (immunohistochemistry)
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Other groups
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
60 µg/kg/day · intraperitoneal · 4 weeksAs given in this study (rat).
Source sentences
Summary
Immunohistochemical analysis revealed no significant differences in collagen type I expression among groups
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
Report a problemFND-4254292607
07AnimalAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, collagen type III expression differed significantly among groups; the direction for TB‑500 is not stated.
In plain wordsIn rats with repaired Achilles tendons, type III collagen amounts differed between groups. The abstract does not say how TB‑500 changed it.
Peptide
TB-500
Outcome
Direction not reportedCollagen type III expression (immunohistochemistry)
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Other groups
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
60 µg/kg/day · intraperitoneal · 4 weeksAs given in this study (rat).
Source sentences
Summary
collagen type III expression differed significantly, consistent with histochemical findings.
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
Report a problemFND-4254292608
08AnimalAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, combined BPC-157 and TB‑500 gave no additional benefit compared with either agent alone.
In plain wordsIn rats with repaired Achilles tendons, using TB‑500 with BPC-157 gave no extra benefit compared with either one alone.
Peptide
TB-500
Outcome
No significant changeAdded benefit of TB-500 plus BPC-157 over single agents
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
TB-500 alone or BPC-157 alone
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
TB-500 60 µg/kg/day with BPC-157 10 µg/kg/day · intraperitoneal · 4 weeksAs given in this study (rat).
Source sentences
Summary
Combined BPC-157 and TB-500 treatment did not confer additional benefits compared to either agent alone.
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
Report a problemFND-4254292609
09AnimalAnimal study (in vivo)Efficacy outcome
In rats after Achilles tendon repair, BPC‑157 gave numerically higher maximum load to failure than control, but the difference was not statistically significant.
In plain wordsIn rats with repaired Achilles tendons, BPC‑157 did not significantly raise the load the tendon could bear compared with control.
Peptide
BPC-157
Outcome
No significant changeMaximum load to failure
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
10 µg/kg/day · intraperitoneal · four weeksAs given in this study (rat).
Source sentences
Summary
Biomechanical testing revealed higher maximum load to failure values in the BPC-157 and TB-500 groups compared to controls, reaching statistical significance in the TB-500 group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Report a problemFND-4254292610
10AnimalAnimal study (in vivo)Efficacy outcome
In rats after Achilles tendon repair, BPC‑157 gave numerically lower total Bonar scores than control, without statistical significance.
In plain wordsIn rats with repaired Achilles tendons, BPC‑157 did not significantly improve tendon tissue scores compared with control.
Peptide
BPC-157
Outcome
No significant changeTotal Bonar score
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
10 µg/kg/day · intraperitoneal · four weeksAs given in this study (rat).
Source sentences
Summary
The BPC-157 group showed numerically lower scores without reaching statistical significance for total scores.
2 context sentences(design, population, supporting numbers)
Summary · context
Histopathological evaluation demonstrated significantly lower total Bonar scores in the TB-500 group (p = 0.016)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Report a problemFND-4254292611
11AnimalAnimal study (in vivo)Efficacy outcome
In rats after Achilles tendon repair, BPC‑157 gave numerically lower total Movin scores than control, without statistical significance.
In plain wordsIn rats with repaired Achilles tendons, BPC‑157 did not significantly improve tendon tissue scores compared with control.
Peptide
BPC-157
Outcome
No significant changeTotal Movin score
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
10 µg/kg/day · intraperitoneal · four weeksAs given in this study (rat).
Source sentences
Summary
The BPC-157 group showed numerically lower scores without reaching statistical significance for total scores.
2 context sentences(design, population, supporting numbers)
Summary · context
and significantly lower total Movin scores in the TB-500 and BPC + TB groups (p = 0.017 and p = 0.040, respectively) relative to controls
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Report a problemFND-4254292612

Studies that did not test the peptide alone6

In this study, the peptide was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking the peptide.

12AnimalNot a test of TB-500 aloneAnimal study (in vivo)Efficacy outcome
In rats with repaired Achilles tendons, TB‑500 combined with BPC-157 lowered the total Movin histopathology score compared with controls (p = 0.040).
In plain wordsIn rats with repaired Achilles tendons, TB‑500 plus BPC-157 gave better tendon tissue scores than no treatment.
Peptide
TB-500
Outcome
DecreaseTotal Movin score (combined with BPC-157)
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Source sentences
Summary
significantly lower total Movin scores in the TB-500 and BPC + TB groups (p = 0.017 and p = 0.040, respectively)
2 context sentences(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
Summary · context
Treatments were administered intraperitoneally for four weeks postoperatively.
In this study, TB-500 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking TB-500.
Report a problemFND-4254292604
13AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Efficacy outcome
In rats after Achilles tendon repair, the combination of BPC‑157 and TB-500 gave significantly lower total Movin scores than control.
In plain wordsIn rats with repaired Achilles tendons, BPC‑157 given together with TB-500 improved tendon tissue scores compared with control.
Peptide
BPC-157
Outcome
DecreaseTotal Movin score with BPC-157 plus TB-500
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Source sentences
Summary
significantly lower total Movin scores in the TB-500 and BPC + TB groups (p = 0.017 and p = 0.040, respectively) relative to controls
1 context sentence(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4254292613
14AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Efficacy outcome
In rats after Achilles tendon repair, BPC‑157 plus TB-500 gave no additional benefit compared with either agent alone.
In plain wordsIn rats with repaired Achilles tendons, giving BPC‑157 with TB-500 was no better than giving either one alone.
Peptide
BPC-157
Outcome
No significant changeCombination versus single agents
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
BPC-157 alone or TB-500 alone
Outcome group
Tendon and ligament healing · Musculoskeletal
Source sentences
Summary
Combined BPC-157 and TB-500 treatment did not confer additional benefits compared to either agent alone.
1 context sentence(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4254292614
15AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Efficacy outcome
In rats after Achilles tendon repair, treatment groups (BPC‑157, TB-500 and combination) showed increased type I collagen organization and altered type III collagen distribution.
In plain wordsIn rats with repaired Achilles tendons, treated groups, mainly the TB-500 group, showed better collagen organization; the result is not specific to BPC‑157.
Peptide
BPC-157
Outcome
MixedSirius red collagen organization
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Control
Outcome group
Tendon and ligament healing · Musculoskeletal
Source sentences
Summary
Sirius red birefringence analysis showed increased type I collagen organization and altered type III collagen distribution in treatment groups, particularly in the TB-500 group, suggesting progression toward matrix maturation.
1 context sentence(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4254292615
16AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Efficacy outcome
In rats after Achilles tendon repair, collagen type I expression did not differ significantly among the groups.
In plain wordsIn rats with repaired Achilles tendons, the amount of collagen type I did not differ between the groups.
Peptide
BPC-157
Outcome
No significant changeCollagen type I expression (immunohistochemistry)
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Other groups
Outcome group
Tendon and ligament healing · Musculoskeletal
Source sentences
Summary
Immunohistochemical analysis revealed no significant differences in collagen type I expression among groups
1 context sentence(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4254292616
17AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Efficacy outcome
In rats after Achilles tendon repair, collagen type III expression differed significantly among the groups.
In plain wordsIn rats with repaired Achilles tendons, the amount of collagen type III differed between the groups.
Peptide
BPC-157
Outcome
Direction not reportedCollagen type III expression (immunohistochemistry)
Study
ratn: not reported in source
Achilles tendon transection and repair
Comparator
Other groups
Outcome group
Tendon and ligament healing · Musculoskeletal
Source sentences
Summary
whereas collagen type III expression differed significantly, consistent with histochemical findings.
1 context sentence(design, population, supporting numbers)
Summary · context
Thirty-two male Sprague-Dawley rats, each aged 12 weeks and weighing approximately 330 g, underwent standardized Achilles tendon transection and repair and were randomly assigned to four groups, with eight rats in each group: control, BPC-157 (10 µg/kg/day), TB-500 (60 µg/kg/day), and combined BPC-1…
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4254292617

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Versions 16 to 41Current
28 Oct 2026, 02:03 UTC
The same notice was added 26 times, first on 13 Oct 2026, 01:52 UTC. The status did not change between them.
Study results updated
Version 15
12 Oct 2026, 18:17 UTC
Study data updated · source update from Europe PMC
Versions 5 to 14
12 Oct 2026, 17:30 UTC
The same notice was added 10 times, first on 9 Oct 2026, 18:17 UTC. The status did not change between them.
Study results updated
Versions 2 to 4
9 Oct 2026, 03:03 UTC
The same notice was added 3 times, first on 9 Oct 2026, 03:02 UTC. The status did not change between them.
Study data updated · source update from Europe PMC
Version 1
9 Oct 2026, 02:56 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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