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Study · Ex vivo tissue · rat · 2004

6 results

Ghrelin and des-acyl ghrelin both inhibit isoproterenol-induced lipolysis in rat adipocytes via a non-type 1a growth hormone secretagogue receptor.

Muccioli G, Pons N, Ghè C, Catapano F, Granata R, Ghigo E

European journal of pharmacology1 Sept 2004Cited by 139 (Europe PMC)

PMID15363972 (opens in a new tab)DOI10.1016/j.ejphar.2004.07.066 (opens in a new tab)PepBankPUB-15363972
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In rat epididymal adipocytes in vitro, hexarelin (1 to 1000 nM) significantly reduced isoproterenol-stimulated glycerol release.

    In plain wordsIn rat fat cells, hexarelin reduced the fat breakdown that isoproterenol triggers.

    Peptide
    Hexarelin
    Outcome
    DecreaseIsoproterenol-stimulated glycerol release (lipolysis)
    Study

    ratn: not reported in source

    Rat epididymal adipocytes and adipose membranes
    Comparator
    Isoproterenol alone
    Outcome group
    Fat metabolism and adipogenesis · Metabolic and weight
    Studied dose
    1-1000 nMAs given in this study (rat).

    Source sentences

    Summary
    Here we show that des-acyl ghrelin, like ghrelin and some synthetic GHS (hexarelin and MK0677) and carboxy-terminally ghrelin fragments such as ghrelin-(1-5) and ghrelin-(1-10), all significantly reduced, over concentrations ranging from 1 to 1000 nM, the stimulation of glycerol release caused in ra…
  2. 02LabEx vivo tissueActivity measurement

    In rat adipocytes, hexarelin was equal in potency to MK0677 and less potent than ghrelin and des-acyl ghrelin on stimulated lipolysis.

    In plain wordsIn rat fat cells, hexarelin worked as strongly as MK0677 and less strongly than ghrelin.

    Peptide
    Hexarelin
    Outcome
    Not applicablePotency on stimulated lipolysis relative to other ligands
    Study

    ratn: not reported in source

    Rat epididymal adipocytes and adipose membranes
    Outcome group
    Receptor activity and mechanism · Metabolic and weight
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The order of potency on stimulated-lipolysis was: des-acyl ghrelin=ghrelin>MK0677=hexarelin>ghrelin-(1-5)=ghrelin-(1-10).
    1 context sentence
    Summary · context
    Here we show that des-acyl ghrelin, like ghrelin and some synthetic GHS (hexarelin and MK0677) and carboxy-terminally ghrelin fragments such as ghrelin-(1-5) and ghrelin-(1-10), all significantly reduced, over concentrations ranging from 1 to 1000 nM, the stimulation of glycerol release caused in ra…
  3. 03LabEx vivo tissueMechanism

    In rat adipose tissue membranes and adipocytes that did not express GHS-R1a mRNA, hexarelin was recognized by a common high-affinity binding site.

    In plain wordsIn rat fat tissue, hexarelin attached to a binding site that is not the usual growth hormone secretagogue receptor.

    Peptide
    Hexarelin
    Outcome
    Not applicableBinding site on adipose tissue membranes and adipocytes
    Study

    ratn: not reported in source

    Rat epididymal adipocytes and adipose membranes
    Outcome group
    Receptor activity and mechanism · Metabolic and weight
    Studied dose
    Dose not stated

    Source sentences

    Summary
    A common high-affinity binding site was recognized in these cells by both acylated and des-acylated ghrelin and also by hexarelin, MK0677, ghrelin-(1-5) and ghrelin-(1-10).
    2 context sentences
    Summary · context
    binding experiments performed on membranes of epididymal adipose tissue or isolated adipocytes that did not express mRNA for GHS-R1a
    Summary · context
    Here we show that des-acyl ghrelin, like ghrelin and some synthetic GHS (hexarelin and MK0677) and carboxy-terminally ghrelin fragments such as ghrelin-(1-5) and ghrelin-(1-10), all significantly reduced, over concentrations ranging from 1 to 1000 nM, the stimulation of glycerol release caused in ra…
  4. 04LabIn vitro (cells)Efficacy outcome

    In rat epididymal adipocytes, MK0677 significantly reduced isoproterenol-stimulated glycerol release over concentrations from 1 to 1000 nM.

    In plain wordsIn cells from rats, MK0677 reduced glycerol release compared with isoproterenol stimulation.

    Peptide
    MK-677 (ibutamoren)
    Outcome
    DecreaseIsoproterenol-stimulated glycerol release
    Study

    ratn: not reported in source

    Rat epididymal adipocytes
    Comparator
    Isoproterenol stimulation
    Outcome group
    Lipids · Metabolic and weight
    Studied dose
    1 to 1000 nMAs given in this study (rat).

    Source sentences

    Summary
    Here we show that des-acyl ghrelin, like ghrelin and some synthetic GHS (hexarelin and MK0677) and carboxy-terminally ghrelin fragments such as ghrelin-(1-5) and ghrelin-(1-10), all significantly reduced, over concentrations ranging from 1 to 1000 nM, the stimulation of glycerol release caused in ra…
  5. 05LabIn vitro (cells)Activity measurement

    In rat adipocytes, MK0677 matched hexarelin in antilipolytic potency, below ghrelin and des-acyl ghrelin and above the tested ghrelin fragments.

    In plain wordsIn cells from rats, MK0677 matched hexarelin, was less potent than ghrelin forms, and was more potent than tested ghrelin fragments.

    Peptide
    MK-677 (ibutamoren)
    Outcome
    MixedAntilipolytic potency ranking
    Study

    ratn: not reported in source

    Rat epididymal adipocytes
    Comparator
    Des-acyl ghrelin, ghrelin, hexarelin, and ghrelin fragments
    Outcome group
    Lipids · Metabolic and weight
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The order of potency on stimulated-lipolysis was: des-acyl ghrelin=ghrelin>MK0677=hexarelin>ghrelin-(1-5)=ghrelin-(1-10).
  6. 06LabIn vitro (cells)Mechanism

    In rat adipocytes, MK0677 recognized a common high-affinity binding site also recognized by the tested ghrelin forms and analogues.

    In plain wordsIn cells from rats, MK0677 recognized a high-affinity binding site shared with tested ghrelin forms and analogues.

    Peptide
    MK-677 (ibutamoren)
    Outcome
    Not applicableCommon high-affinity binding site recognition
    Study

    ratn: not reported in source

    Rat epididymal adipocytes
    Outcome group
    Receptor activity and mechanism · Metabolic and weight
    Studied dose
    Dose not stated

    Source sentences

    Summary
    A common high-affinity binding site was recognized in these cells by both acylated and des-acylated ghrelin and also by hexarelin, MK0677, ghrelin-(1-5) and ghrelin-(1-10).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    14 Oct 2026, 01:46 UTC

    Study data updated · source update from Europe PMC

  2. Version 2

    13 Oct 2026, 21:44 UTC

    Study results updated

  3. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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