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Study · In vitro (cells) · 2002

6 results

Natural (ghrelin) and synthetic (hexarelin) GH secretagogues stimulate H9c2 cardiomyocyte cell proliferation.

Pettersson I, Muccioli G, Granata R, Deghenghi R, Ghigo E, Ohlsson C, Isgaard J

The Journal of endocrinology1 Oct 2002Cited by 75 (Europe PMC)

PMID12379504 (opens in a new tab)DOI10.1677/joe.0.1750201 (opens in a new tab)PepBankPUB-12379504
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In H9c2 cardiomyocytes, hexarelin increased thymidine incorporation in a dose-dependent manner, with a significant response from 3 micro M.

    In plain wordsIn heart muscle cells in lab dishes, hexarelin increased a marker of cell growth as the dose went up.

    Peptide
    Hexarelin
    Outcome
    IncreaseThymidine incorporation (dose response)
    Study

    n: not reported in source

    H9c2 cardiomyocyte cell line
    Comparator
    Control
    Outcome group
    Cell proliferation and differentiation · Cardiovascular
    Studied dose
    3 micro M to about 30 micro MAs given in this study.

    Measurements

    Thymidine incorporation
    147 % of control
    3 micro M

    Source sentences

    Summary
    Hexarelin stimulated thymidine incorporation in a dose-dependent manner with significant responses at 3 micro M (147+/-3% of control, P<0.01) and elicited maximal effects at concentrations around 30 micro M.
    1 context sentence
    Summary · context
    The aim of the present study was to investigate if the natural ligand ghrelin and synthetic GHS peptide hexarelin and analogues have direct effects on the cardiomyocyte cell line, H9c2.
  2. 02LabIn vitro (cells)Efficacy outcome

    In H9c2 cardiomyocytes, hexarelin increased thymidine incorporation after 12 h, with a maximal effect after 18 h.

    In plain wordsIn heart muscle cells in lab dishes, hexarelin raised a marker of cell growth after 12 hours, most strongly after 18 hours.

    Peptide
    Hexarelin
    Outcome
    IncreaseThymidine incorporation (time course)
    Study

    n: not reported in source

    H9c2 cardiomyocyte cell line
    Comparator
    Control
    Outcome group
    Cell proliferation and differentiation · Cardiovascular
    Studied dose
    Dose not stated · 12 h to 18 hAs given in this study.

    Measurements

    Thymidine incorporation
    176 % of control
    18 h

    Source sentences

    Summary
    This activity was seen already after 12 h of incubation with a maximal effect after 18 h (176+/-9% of control, P<0.01).
    1 context sentence
    Summary · context
    The aim of the present study was to investigate if the natural ligand ghrelin and synthetic GHS peptide hexarelin and analogues have direct effects on the cardiomyocyte cell line, H9c2.
  3. 03LabIn vitro (cells)Mechanism

    In H9c2 cells, the stimulation by hexarelin was specific: truncated peptide EP51389 and MK-0677 had no stimulatory effect.

    In plain wordsIn heart muscle cells in lab dishes, hexarelin raised cell growth, while two related compounds did not.

    Peptide
    Hexarelin
    Outcome
    IncreaseSpecificity of thymidine incorporation
    Study

    n: not reported in source

    H9c2 cardiomyocyte cell line
    Comparator
    Control
    Outcome group
    Cell proliferation and differentiation · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The stimulatory effect on thymidine incorporation of hexarelin, Tyr-Ala-hexarelin, EP80317 and ghrelin was specific and no stimulatory effect was observed with the truncated GH-releasing peptide EP51389 or the non-peptidyl GHS MK-0677.
    1 context sentence
    Summary · context
    The aim of the present study was to investigate if the natural ligand ghrelin and synthetic GHS peptide hexarelin and analogues have direct effects on the cardiomyocyte cell line, H9c2.
  4. 04LabIn vitro (cells)Activity measurement

    In H9c2 membrane binding studies, hexarelin displaced the radioligand (125)I-Tyr-Ala-hexarelin, while MK-0677 and EP51389 displaced very little.

    In plain wordsIn lab tests on heart cell membranes, hexarelin competed for a binding site, while two other compounds hardly did.

    Peptide
    Hexarelin
    Outcome
    Not applicableDisplacement of radioligand binding
    Study

    n: not reported in source

    H9c2 cardiomyocyte cell line
    Comparator
    Control
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    competition curves showed displacement with hexarelin, Tyr-Ala-hexarelin, EP80317 and ghrelin
    1 context sentence
    Summary · context
    The aim of the present study was to investigate if the natural ligand ghrelin and synthetic GHS peptide hexarelin and analogues have direct effects on the cardiomyocyte cell line, H9c2.
  5. 05LabIn vitro (cells)Efficacy outcome

    In H9c2 cardiomyocytes, MK-0677 did not stimulate thymidine incorporation.

    In plain wordsIn cells, MK-0677 did not stimulate thymidine incorporation in H9c2 heart cells.

    Peptide
    MK-677 (ibutamoren)
    Outcome
    No significant changeThymidine incorporation
    Study

    n: not reported in source

    H9c2 cardiomyocyte cell line
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The stimulatory effect on thymidine incorporation of hexarelin, Tyr-Ala-hexarelin, EP80317 and ghrelin was specific and no stimulatory effect was observed with the truncated GH-releasing peptide EP51389 or the non-peptidyl GHS MK-0677.
    1 context sentence
    Summary · context
    The aim of the present study was to investigate if the natural ligand ghrelin and synthetic GHS peptide hexarelin and analogues have direct effects on the cardiomyocyte cell line, H9c2.
  6. 06LabIn vitro (cells)Activity measurement

    In H9c2 cardiomyocyte binding assays, MK-0677 produced very little displacement of radiolabeled Tyr-Ala-hexarelin at 1 micro M.

    In plain wordsIn cells, MK-0677 produced very little displacement of the binding tracer in H9c2 heart cells.

    Peptide
    MK-677 (ibutamoren)
    Outcome
    Not applicableRadioligand displacement
    Study

    n: not reported in source

    H9c2 cardiomyocyte cell line
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    1 micro MAs given in this study.

    Source sentences

    Summary
    In competitive binding studies, (125)I-labeled Tyr-Ala-hexarelin was used as radioligand and competition curves showed displacement with hexarelin, Tyr-Ala-hexarelin, EP80317 and ghrelin, whereas MK-0677 and EP51389 produced very little displacement at 1 micro M concentration,
    1 context sentence
    Summary · context
    The aim of the present study was to investigate if the natural ligand ghrelin and synthetic GHS peptide hexarelin and analogues have direct effects on the cardiomyocyte cell line, H9c2.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    14 Oct 2026, 01:46 UTC

    Study data updated · source update from Europe PMC

  2. Version 2

    13 Oct 2026, 21:44 UTC

    Study results updated

  3. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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