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Study · Ex vivo tissue · rat · 1999

11 results

Identification and characterization of a new growth hormone-releasing peptide receptor in the heart.

Bodart V, Bouchard JF, McNicoll N, Escher E, Carrière P, Ghigo E, Sejlitz T, Sirois MG, Lamontagne D, Ong H

Circulation research1 Oct 1999Cited by 74 (Europe PMC)

PMID10532947 (opens in a new tab)DOI10.1161/01.res.85.9.796 (opens in a new tab)PepBankPUB-10532947
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In perfused rat hearts, hexarelin induced a dose-dependent increase in coronary perfusion pressure.

    In plain wordsIn isolated rat hearts, hexarelin raised the pressure needed to push fluid through the heart's blood vessels, more at higher doses.

    Peptide
    Hexarelin
    Outcome
    IncreaseCoronary perfusion pressure
    Study

    ratn: not reported in source

    Langendorff perfused rat heart
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated · perfusionAs given in this study (rat).

    Source sentences

    Summary
    In the Langendorff rat heart system, hexarelin induced a dose-dependent increase in coronary perfusion pressure.
    1 context sentence
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.
  2. 02LabEx vivo tissueActivity measurement

    In rat cardiac membranes, MK-0677 did not displace the photoactivatable hexarelin derivative.

    In plain wordsIn lab tests, MK-0677 did not displace the labeled hexarelin derivative from rat heart membranes.

    Peptide
    MK-677 (ibutamoren)
    Outcome
    No significant changePhotoactivatable radioligand displacement
    Study

    ratn: not reported in source

    rat cardiac membranes
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    MK-0677 and EP51389, both potent GH secretagogues, did not displace the binding of the photoactivatable derivative from rat cardiac membranes.
  3. 03LabEx vivo tissueEfficacy outcome

    In isolated rat hearts, MK-0677 showed no vasoconstrictive activity.

    In plain wordsIn lab tests, MK-0677 did not constrict blood vessels in isolated rat hearts.

    Peptide
    MK-677 (ibutamoren)
    Outcome
    No significant changeVasoconstrictive activity
    Study

    ratn: not reported in source

    Langendorff rat hearts
    Outcome group
    Heart function · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, both compounds were devoid of any vasoconstrictive activity.
    2 context sentences
    Summary · context
    MK-0677 and EP51389, both potent GH secretagogues, did not displace the binding of the photoactivatable derivative from rat cardiac membranes.
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.
  4. 04LabIn vitro (cell-free)Activity measurement

    In rat cardiac membranes, hexarelin competed for binding of the photoactivatable derivative with an IC50 of 2.9 micromol/L.

    In plain wordsIn lab tests on rat heart membranes, hexarelin competed with a labeled probe for binding with an IC50 of 2.9 micromol/L.

    Peptide
    Hexarelin
    Outcome
    Not applicableHexarelin competition binding in cardiac membranes
    Study

    ratn: not reported in source

    Rat cardiac membranes
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Measurements

    IC50
    2.9 micromol/L
    rat cardiac membranes

    Source sentences

    Summary
    Competition binding studies gave an IC(50) of 2.9 micromol/L for hexarelin
    2 context sentences
    Summary · context
    To characterize the hexarelin binding sites in the rat heart, [(125)I]Tyr-Bpa-Ala-hexarelin was used as photoactivatable radioligand in saturation and competitive binding studies.
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.

Studies that did not test the peptide alone7

In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Hexarelin.

  1. 05LabNot a test of Hexarelin aloneEx vivo tissueMechanism

    In perfused rat hearts, nifedipine partially inhibited the vasoconstriction induced by hexarelin, which suggests a role for L-type Ca2+ channels.

    In plain wordsIn isolated rat hearts, nifedipine partly blocked the blood vessel narrowing caused by hexarelin.

    Peptide
    Hexarelin
    Outcome
    DecreaseNifedipine effect on hexarelin vasoconstriction
    Study

    ratn: not reported in source

    Langendorff perfused rat heart
    Outcome group
    Receptor activity and mechanism · Cardiovascular

    Source sentences

    Summary
    Nifedipine, chelerythrine, and bisindolylmaleimide partially inhibited the vasoconstriction induced by hexarelin, suggesting that this effect was mediated at least in part by L-type Ca(2+) channels and protein kinase C.
    1 context sentence
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.

    In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.

  2. 06LabNot a test of Hexarelin aloneEx vivo tissueMechanism

    In perfused rat hearts, chelerythrine partially inhibited the vasoconstriction induced by hexarelin, which suggests a role for protein kinase C.

    In plain wordsIn isolated rat hearts, chelerythrine partly blocked the blood vessel narrowing caused by hexarelin.

    Peptide
    Hexarelin
    Outcome
    DecreaseChelerythrine effect on hexarelin vasoconstriction
    Study

    ratn: not reported in source

    Langendorff perfused rat heart
    Outcome group
    Receptor activity and mechanism · Cardiovascular

    Source sentences

    Summary
    Nifedipine, chelerythrine, and bisindolylmaleimide partially inhibited the vasoconstriction induced by hexarelin, suggesting that this effect was mediated at least in part by L-type Ca(2+) channels and protein kinase C.
    1 context sentence
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.

    In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.

  3. 07LabNot a test of Hexarelin aloneEx vivo tissueMechanism

    In perfused rat hearts, bisindolylmaleimide partially inhibited the vasoconstriction induced by hexarelin, which suggests a role for protein kinase C.

    In plain wordsIn isolated rat hearts, bisindolylmaleimide partly blocked the blood vessel narrowing caused by hexarelin.

    Peptide
    Hexarelin
    Outcome
    DecreaseBisindolylmaleimide effect on hexarelin vasoconstriction
    Study

    ratn: not reported in source

    Langendorff perfused rat heart
    Outcome group
    Receptor activity and mechanism · Cardiovascular

    Source sentences

    Summary
    Nifedipine, chelerythrine, and bisindolylmaleimide partially inhibited the vasoconstriction induced by hexarelin, suggesting that this effect was mediated at least in part by L-type Ca(2+) channels and protein kinase C.
    1 context sentence
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.

    In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.

  4. 08LabNot a test of Hexarelin aloneEx vivo tissueMechanism

    In perfused rat hearts, diclofenac did not change the coronary vasoconstriction induced by hexarelin.

    In plain wordsIn isolated rat hearts, diclofenac had no effect on the blood vessel narrowing caused by hexarelin.

    Peptide
    Hexarelin
    Outcome
    No significant changeDiclofenac effect on hexarelin vasoconstriction
    Study

    ratn: not reported in source

    Langendorff perfused rat heart
    Outcome group
    Receptor activity and mechanism · Cardiovascular

    Source sentences

    Summary
    In contrast, diclofenac and 1-(7-carboxyheptyl)imidazole were without effect, suggesting that prostaglandins and thromboxanes were not involved in the coronary vasoconstriction induced by hexarelin.
    1 context sentence
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.

    In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.

  5. 09LabNot a test of Hexarelin aloneEx vivo tissueMechanism

    In perfused rat hearts, 1-(7-carboxyheptyl)imidazole did not change the coronary vasoconstriction induced by hexarelin.

    In plain wordsIn isolated rat hearts, 1-(7-carboxyheptyl)imidazole had no effect on the blood vessel narrowing caused by hexarelin.

    Peptide
    Hexarelin
    Outcome
    No significant change1-(7-carboxyheptyl)imidazole effect on hexarelin vasoconstriction
    Study

    ratn: not reported in source

    Langendorff perfused rat heart
    Outcome group
    Receptor activity and mechanism · Cardiovascular

    Source sentences

    Summary
    In contrast, diclofenac and 1-(7-carboxyheptyl)imidazole were without effect, suggesting that prostaglandins and thromboxanes were not involved in the coronary vasoconstriction induced by hexarelin.
    1 context sentence
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.

    In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.

  6. 10LabNot a test of Hexarelin aloneIn vitro (cell-free)Mechanism

    In rat cardiac membranes, a photoactivatable hexarelin derivative specifically labeled a hexarelin receptor with a molecular weight of 84 000.

    In plain wordsIn lab tests on rat heart membranes, a hexarelin-like probe labeled a receptor with a size of 84 000.

    Peptide
    Hexarelin
    Outcome
    Not applicableHexarelin receptor labelled in cardiac membranes
    Study

    ratn: not reported in source

    Rat cardiac membranes
    Outcome group
    Receptor activity and mechanism · Cardiovascular

    Source sentences

    Summary
    We specifically labeled a hexarelin receptor with an M(r) of 84 000 in rat cardiac membranes.
    2 context sentences
    Summary · context
    To characterize the hexarelin binding sites in the rat heart, [(125)I]Tyr-Bpa-Ala-hexarelin was used as photoactivatable radioligand in saturation and competitive binding studies.
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.

    In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.

  7. 11LabNot a test of Hexarelin aloneIn vitro (cell-free)Mechanism

    In rat cardiac membranes, saturation binding of the hexarelin derivative showed a single class of binding sites with a Kd of 14.5 nmol/L and a density of 91 fmol/mg of protein.

    In plain wordsIn lab tests on rat heart membranes, a hexarelin-like probe bound to one type of site with a Kd of 14.5 nmol/L.

    Peptide
    Hexarelin
    Outcome
    Not applicableRadioligand saturation binding in cardiac membranes
    Study

    ratn: not reported in source

    Rat cardiac membranes
    Outcome group
    Receptor activity and mechanism · Cardiovascular

    Measurements

    Kd
    14.5 nmol/L
    rat cardiac membranes
    Binding site density
    91 fmol/mg protein
    rat cardiac membranes

    Source sentences

    Summary
    Saturation binding curves revealed a single class of binding sites with a K(d) of 14.5 nmol/L and a density of 91 fmol/mg of protein.
    2 context sentences
    Summary · context
    To characterize the hexarelin binding sites in the rat heart, [(125)I]Tyr-Bpa-Ala-hexarelin was used as photoactivatable radioligand in saturation and competitive binding studies.
    Summary · context
    To document whether hexarelin action is mediated through specific cardiac receptors, perfusion of Langendorff rat hearts with hexarelin and binding studies were carried out.

    In this study, Hexarelin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Hexarelin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    14 Oct 2026, 01:46 UTC

    Study data updated · source update from Europe PMC

  2. Version 2

    13 Oct 2026, 21:44 UTC

    Study results updated

  3. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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