Effect of Adrenocorticotropic Hormone on Vascular Endothelial Growth Factor Release in Children Study

Status

Completed

Phase

Early phase 1

Enrollment

10 participants (actual)

Sponsor

Nationwide Children's Hospital · Other

Design

Allocation not applicable · Open label (no masking)

Countries

1 country

United States

Start

1 Oct 2017

Primary completion

31 Oct 2018

Completion

31 Jan 2019

First posted

17 Oct 2018

Last update posted

21 Feb 2019

Conditions

Adrenal Insufficiency · Osteopenia, Osteoporosis · Steroid Suppression of ACTH Secretion

Bone disease and adrenal suppression are two of the many side effects of steroid use in pediatrics. Evidence has shown that adrenocorticotropic hormone (ACTH) protects against the adverse bone effects of steroids in animals and in vitro models, but this has not yet been evaluated in humans. The proposed mechanism in these studies is that ACTH stimulates osteoblasts in bone to release Vascular Endothelial Growth Factor (VEGF), which increases the vascularity in high risk areas of bone. This can potentially be protective against osteonecrosis and osteopenia, which can lead to bone fractures if not prevented. The VEGF release can also be used to demonstrate that an administration of exogenous ACTH occurred. This could be important in diagnosing adrenal insufficiency (AI). One of the tests to assess central AI is the low-dose ACTH stimulation test (LDAST). This test has a high rate of false positive results due to technical limitations. However, if an ACTH-stimulated VEGF level can be measured during the test as a marker of the test being done properly, it will allow for proper interpretation of the results (and identification of a false positive), which will reduce the number of patients being incorrectly diagnosed with central AI. This study will recruit ten healthy children and adolescents, ages 9-18, to assess the effects of ACTH on VEGF levels. The investigators will measure the response of VEGF and cortisol to an administration of a low dose and high dose of cosyntropin (the synthetic ACTH analog used in this test). The hypothesis of this study is that VEGF and cortisol will both increase after administration of cosyntropin. At this time, no other studies have demonstrated that VEGF is responsive to ACTH in humans. If the hypothesis is correct, the results will have two main implications. VEGF can be used as a marker of ACTH administration during the LDAST to identify false positive tests. Secondly, this will help further research into whether ACTH can be used to protect against bone disease in high-dose steroid-treated patients. Further studies can be done to assess whether this effect will be the same in patients with AI or steroid-induced adrenal suppression.

• Experimental

  ACTH stim test arm

  Cosyntropin 1 mcg IV (low dose) will be given to subjects at t=0 minutes, and Cosyntropin 250 mcg (high dose) IV will be given to subjects at t=60 minutes. (All subjects were in the same arm and had the same protocol).

1. Difference between VEGF levels at baseline and the peak VEGF level after low dose cosyntropin administration in healthy children and adolescents

   Time frame · From t=0 minutes to t=60 minutes (1 hour total)

   Registry description

   Prior to administering Cosyntropin, a VEGF level will be obtained. 1 mcg Cosyntropin will be administered at t=0, then VEGF will be measured at 30 and 60 minutes. The investigators will run a comparison on the peak plasma VEGF level (the higher of the 30 and 60 minute level) after low dose stimulation compared to baseline VEGF levels.

No study cites this trial.