Detection of Mitochondrial‐Derived Peptide Humanin in Semen and Reproductive Tract of Caprine Along With Its Relation to Seasonality

Chouksey S, Shukla SN, Dubey A, Soni Y, Mandal S, Chourasia A

Reproduction in Domestic Animals1 Aug 2025

PepBankPUB-90668108

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Summary

The text of this summary is not shown here, because the article license does not allow reuse.

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Natural levels measured5

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In Sirohi and Barbari bucks, immunofluorescence detected humanin in the elongated spermatids of the caput epididymis.
In plain wordsIn male goats, natural humanin was found in developing sperm cells in part of the epididymis.
Peptide
Humanin
Outcome
Not applicableHumanin in elongated spermatids of the caput epididymis
Study
goatn = 12
Sirohi and Barbari buck reproductive tract and semen
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Immunofluorescence examination of the male reproductive tract revealed the presence of green fluorescence, indicating humanin, in the elongated spermatids of the caput epididymis and the interstitial space of the testicles.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 12 bucks were selected, and an indirect immunofluorescence test was conducted to detect humanin using a commercially available anti‐humanin antibody.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-9066810801
02AnimalEndogenous levelAnimal study (in vivo)Other
In Sirohi and Barbari bucks, immunofluorescence detected humanin in the interstitial space of the testicles.
In plain wordsIn male goats, natural humanin was found in the tissue spaces between testicular structures.
Peptide
Humanin
Outcome
Not applicableHumanin in testicular interstitial space
Study
goatn = 12
Sirohi and Barbari buck reproductive tract and semen
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Immunofluorescence examination of the male reproductive tract revealed the presence of green fluorescence, indicating humanin, in the elongated spermatids of the caput epididymis and the interstitial space of the testicles.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 12 bucks were selected, and an indirect immunofluorescence test was conducted to detect humanin using a commercially available anti‐humanin antibody.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-9066810802
03AnimalEndogenous levelAnimal study (in vivo)Other
In ejaculated buck spermatozoa, humanin was localised in the neck and acrosomal regions.
In plain wordsIn male goats, natural humanin was found in the neck and tip regions of ejaculated sperm.
Peptide
Humanin
Outcome
Not applicableHumanin location in ejaculated spermatozoa (neck and acrosome)
Study
goatn = 12
Sirohi and Barbari buck reproductive tract and semen
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
In ejaculated spermatozoa, humanin was localised in the neck and acrosomal regions.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 12 bucks were selected, and an indirect immunofluorescence test was conducted to detect humanin using a commercially available anti‐humanin antibody.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-9066810803
04AnimalEndogenous levelAnimal study (in vivo)Other
In ejaculated buck spermatozoa, humanin was also observed in the upper middle region.
In plain wordsIn male goats, natural humanin was also seen in the upper middle part of ejaculated sperm.
Peptide
Humanin
Outcome
Not applicableHumanin location in ejaculated spermatozoa (upper middle region)
Study
goatn = 12
Sirohi and Barbari buck reproductive tract and semen
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Humanin was also observed in the upper middle region of ejaculated spermatozoa.
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 12 bucks were selected, and an indirect immunofluorescence test was conducted to detect humanin using a commercially available anti‐humanin antibody.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-9066810804
05AnimalEndogenous levelAnimal study (in vivo)Other
In bucks, humanin immunofluorescence was stronger in the rainy season than in summer and winter.
In plain wordsIn male goats, natural humanin signal was stronger in the rainy season than in summer and winter.
Peptide
Humanin
Outcome
IncreaseHumanin expression in the rainy season
Study
goatn = 12
Sirohi and Barbari buck reproductive tract and semen
Comparator
Summer and winter
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
during the rainy season, humanin expression was stronger or brighter
1 context sentence(design, population, supporting numbers)
Summary · context
A total of 12 bucks were selected, and an indirect immunofluorescence test was conducted to detect humanin using a commercially available anti‐humanin antibody.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-9066810805

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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