Efficient Expression of Small Molecule Bioactive Peptides in Bacillus licheniformis.

Zhang H, Shao L, Feng Q, Guo Q, Wang H, Zhang H

Applied biochemistry and biotechnology29 Aug 2026

PMID42667535DOI10.1007/s12010-026-05885-6PepBankPUB-42667535

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone8

In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking BPC-157.

01AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Efficacy outcome
In rats with ethanol-induced gastric ulcer, the BPC157 fusion proteins given as protection showed ulcer inhibition rates of 75.3% to 82.9%.
In plain wordsIn rats, BPC157 fusion proteins given before ethanol reduced stomach ulcers.
Peptide
BPC-157
Outcome
IncreaseUlcer inhibition, protective regime
Study
ratn: not reported in source
Rat ethanol-induced acute gastric ulcer
Outcome group
Gastrointestinal protection · Gastrointestinal
Measurements
Ulcer inhibition rate
75.3 to 82.9%
protective regime
Source sentences
Summary
Both proteins showed gastroprotective and therapeutic activity, with ulcer inhibition rates ranging from 75.3% to 82.9% protective regime
1 context sentence(design, population, supporting numbers)
Summary · context
Biological activity was assessed in a rat model of ethanol-induced acute gastric ulcer.
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4266753503
02AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Efficacy outcome
In rats with ethanol-induced gastric ulcer, the BPC157 fusion proteins given as treatment showed ulcer inhibition rates of 80.4% to 84.6%.
In plain wordsIn rats, BPC157 fusion proteins given after ethanol reduced stomach ulcers.
Peptide
BPC-157
Outcome
IncreaseUlcer inhibition, therapeutic regime
Study
ratn: not reported in source
Rat ethanol-induced acute gastric ulcer
Outcome group
Gastrointestinal protection · Gastrointestinal
Measurements
Ulcer inhibition rate
80.4 to 84.6%
therapeutic regime
Source sentences
Summary
Both proteins showed gastroprotective and therapeutic activity, with ulcer inhibition rates ranging from 75.3% to 82.9% protective regime and 80.4% to 84.6% therapeutic regime.
1 context sentence(design, population, supporting numbers)
Summary · context
Biological activity was assessed in a rat model of ethanol-induced acute gastric ulcer.
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4266753504
03AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Efficacy outcome
In rats with ethanol-induced gastric ulcer, treatment with the BPC157 fusion proteins reduced mucosal inflammation and stimulated glandular repair on histology.
In plain wordsIn rats, treatment with BPC157 fusion proteins lessened stomach lining inflammation and helped repair.
Peptide
BPC-157
Outcome
DecreaseMucosal inflammation and glandular repair
Study
ratn: not reported in source
Rat ethanol-induced acute gastric ulcer
Outcome group
Gastrointestinal protection · Gastrointestinal
Source sentences
Summary
Histological analysis showed that the treatment reduced mucosal inflammation and stimulated glandular repair.
1 context sentence(design, population, supporting numbers)
Summary · context
Biological activity was assessed in a rat model of ethanol-induced acute gastric ulcer.
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4266753505
04AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Mechanism
In rats with ethanol-induced gastric ulcer, the BPC157 fusion proteins significantly downregulated TNF-α in gastric tissue.
In plain wordsIn rats, BPC157 fusion proteins lowered the inflammation marker TNF-α in the stomach.
Peptide
BPC-157
Outcome
DecreaseGastric TNF-α
Study
ratn: not reported in source
Rat ethanol-induced acute gastric ulcer
Outcome group
Inflammation markers · Gastrointestinal
Source sentences
Summary
ELISA showed significant downregulation of proinflammatory cytokines TNF-α, IL-1β, and IL-6 in gastric tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Biological activity was assessed in a rat model of ethanol-induced acute gastric ulcer.
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4266753506
05AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Mechanism
In rats with ethanol-induced gastric ulcer, the BPC157 fusion proteins significantly downregulated IL-1β in gastric tissue.
In plain wordsIn rats, BPC157 fusion proteins lowered the inflammation marker IL-1β in the stomach.
Peptide
BPC-157
Outcome
DecreaseGastric IL-1β
Study
ratn: not reported in source
Rat ethanol-induced acute gastric ulcer
Outcome group
Inflammation markers · Gastrointestinal
Source sentences
Summary
ELISA showed significant downregulation of proinflammatory cytokines TNF-α, IL-1β, and IL-6 in gastric tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Biological activity was assessed in a rat model of ethanol-induced acute gastric ulcer.
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4266753507
06AnimalNot a test of BPC-157 aloneAnimal study (in vivo)Mechanism
In rats with ethanol-induced gastric ulcer, the BPC157 fusion proteins significantly downregulated IL-6 in gastric tissue.
In plain wordsIn rats, BPC157 fusion proteins lowered the inflammation marker IL-6 in the stomach.
Peptide
BPC-157
Outcome
DecreaseGastric IL-6
Study
ratn: not reported in source
Rat ethanol-induced acute gastric ulcer
Outcome group
Inflammation markers · Gastrointestinal
Source sentences
Summary
ELISA showed significant downregulation of proinflammatory cytokines TNF-α, IL-1β, and IL-6 in gastric tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Biological activity was assessed in a rat model of ethanol-induced acute gastric ulcer.
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4266753508
07LabNot a test of BPC-157 aloneIn vitro (cells)Other
In Bacillus licheniformis, reporter-based expression signals of BPC157 fusion proteins rose about threefold under optimal conditions compared with the basal medium.
In plain wordsIn bacteria, the best growth conditions raised production of the BPC157 fusion proteins about threefold compared with basic medium.
Peptide
BPC-157
Outcome
IncreaseReporter expression signal under optimized fermentation
Study
Bacillus licheniformisn: not reported in source
Bacillus licheniformis strain 2709 expressing fusion proteins
Comparator
Basal medium
Outcome group
Synthesis and manufacturing · Pharmacology
Source sentences
Summary
Under optimal conditions (2% inoculum, 40 g/L soybean peptone, and 80 g/L glucose), reporter-based expression signals increased approximately threefold compared to the basal medium.
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4266753501
08LabNot a test of BPC-157 aloneIn vitro (cells)Other
The fusion proteins were purified by fractional ammonium sulfate precipitation, with optimal saturations of 50% and 60%.
In plain wordsIn the lab, the BPC157 fusion proteins were purified best at 50% and 60% salt saturation.
Peptide
BPC-157
Outcome
Not applicableOptimal ammonium sulfate saturation for purification
Study
Bacillus licheniformisn: not reported in source
Bacillus licheniformis strain 2709 expressing fusion proteins
Outcome group
Synthesis and manufacturing · Pharmacology
Measurements
Optimal ammonium sulfate saturation
50 to 60%
fusion proteins
Source sentences
Summary
The fusion proteins were purified by fractional ammonium sulfate precipitation; optimal saturations were 50% and 60%, respectively.
In this study, BPC-157 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking BPC-157.
Report a problemFND-4266753502

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 3Current
10 Oct 2026, 08:19 UTC
Study results updated
Version 2
9 Oct 2026, 03:03 UTC
Study data updated · source update from Europe PMC
Version 1
9 Oct 2026, 02:56 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

Keywords

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