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Study · Not a test of the peptide alone · 2026

2 results

Network Topology and Interactomic Analysis Reveal the Regulatory Framework of the Humanin Protein Family (MTRNR2Lx Class).

Shahzaib M, Aprile D, Laporta G, Galderisi U, Colonna G

Biomolecules3 Jul 2026

PMID42509775 (opens in a new tab)PMCIDPMC13406609 (opens in a new tab)DOI10.3390/biom16070981 (opens in a new tab)PepBankPUB-42509775
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Summary

This study presents an in-depth analysis of an interactome comprising approximately 1033 nodes, focusing on its topology, reliability, and functional implications, with particular attention to the small mitochondrial proteins of the Humanin family and their nuclear-encoded MTRNR2Lx paralogs. The analysis, conducted through stringent high-reliability filters and experimentally supported interaction data, produced a curated network model in which approximately 70% of the retained interactions were supported by experimental evidence, providing a solid basis for network-based functional interpretation. The topology of the interactome showed scale-free and modular network characteristics, with hub and bottleneck nodes defining highly connected stress-response and regulatory modules. Humanin-related proteins, positioned at the periphery of the interactome, emerged as candidate modulatory nodes linking peripheral signaling interfaces to broader functional modules. Mitochondrial Humanin may contribute to early cytoprotective responses, including pathways associated with BAX-dependent apoptosis regulation, whereas nuclear MTRNR2Lx proteins appear to be connected to more sustained regulatory networks involving neuroprotection- and apoptosis-associated modules under chronic stress conditions. In particular, the MTRNR2Lx-FPR2/G-protein module, including GNB1, emerged as a candidate signaling interface that may contribute to the downstream organization of Humanin-related responses. This network-based distinction supports the view that Humanin-family peptides may operate as modulators of stress-response networks rather than as isolated effectors of intrinsic mitochondrial functions. Overall, the methodological approach, results, and proposed model provide new insights into the systems-level organization of Humanin biology and identify prioritized molecular candidates for future in vitro and in vivo validation in the context of neurodegeneration, apoptosis, and cellular stress.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone2

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 01Computer modelNot a test of Humanin aloneIn silicoMechanism

    In a predicted interactome of about 1033 nodes, Humanin-related proteins sat at the periphery and emerged as candidate modulatory nodes.

    In plain wordsA computer model of protein networks placed Humanin-related proteins at the edge and flagged them as possible modulators.

    Peptide
    Humanin
    Outcome
    Not applicablePosition of Humanin-related proteins in the interactome
    Study

    n: not reported in source

    Protein interaction network (about 1033 nodes)
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Humanin-related proteins, positioned at the periphery of the interactome, emerged as candidate modulatory nodes linking peripheral signaling interfaces to broader functional modules.
    1 context sentence
    Summary · context
    This study presents an in-depth analysis of an interactome comprising approximately 1033 nodes

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 02Computer modelNot a test of Humanin aloneIn silicoMechanism

    In the predicted interactome, the MTRNR2Lx-FPR2/G-protein module, including GNB1, emerged as a candidate signaling interface for Humanin-related responses.

    In plain wordsA computer model flagged a protein module including GNB1 as a possible signaling link for Humanin-related responses.

    Peptide
    Humanin
    Outcome
    Not applicableMTRNR2Lx-FPR2/G-protein module
    Study

    n: not reported in source

    Protein interaction network (about 1033 nodes)
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    In particular, the MTRNR2Lx-FPR2/G-protein module, including GNB1, emerged as a candidate signaling interface that may contribute to the downstream organization of Humanin-related responses.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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