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Study · Animal study (in vivo) · mouse · 2026

10 results

S14G-humanin (HNG) ameliorates diabetic nephropathy tubular injury by inhibiting Z-DNA/ZBP1-mediated necroptosis.

Liu Y, Hu C, Luo S, Han Y, Li L, Wei L, Chen W, Yang M

European journal of pharmacology23 Jul 2026Cited by 1 (Europe PMC)

PMID42492881 (opens in a new tab)DOI10.1016/j.ejphar.2026.179175 (opens in a new tab)PepBankPUB-42492881
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice with diabetic nephropathy, S14G-humanin dose-dependently alleviated renal injury.

    In plain wordsIn mice with diabetic nephropathy, S14G-humanin reduced kidney injury, and a higher dose worked better.

    Peptide
    Humanin
    Outcome
    DecreaseRenal injury (dose-dependent)
    Study

    mousen: not reported in source

    Diabetic nephropathy mice (high-fat diet plus streptozotocin)
    Comparator
    Untreated DN mice
    Outcome group
    Kidney function · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG treatment dose-dependently alleviated renal injury in DN mice.
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In mice with diabetic nephropathy, high-dose S14G-humanin reduced the urinary albumin-to-creatinine ratio by 49.5% versus untreated DN mice.

    In plain wordsIn mice with diabetic nephropathy, high-dose S14G-humanin lowered urine albumin-to-creatinine ratio compared with untreated mice.

    Peptide
    Humanin
    Outcome
    DecreaseUrinary albumin-to-creatinine ratio
    Study

    mousen: not reported in source

    Diabetic nephropathy mice (high-fat diet plus streptozotocin)
    Comparator
    Untreated DN mice
    Outcome group
    Kidney function · Kidney and urinary
    Studied dose
    High doseAs given in this study (mouse).

    Measurements

    UACR reduction
    49.5%
    high-dose HNG

    Source sentences

    Summary
    Specifically, high-dose-HNG significantly reduced the urinary albumin-to-creatinine ratio (UACR) by 49.5% (from 103.91 ± 7.68 to 52.50 ± 4.02 μg/mg, P < 0.001) and alleviated renal pathological injury compared to untreated DN mice.
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In mice with diabetic nephropathy, high-dose S14G-humanin alleviated renal pathological injury versus untreated DN mice.

    In plain wordsIn mice with diabetic nephropathy, high-dose S14G-humanin reduced kidney tissue damage compared with untreated mice.

    Peptide
    Humanin
    Outcome
    DecreaseRenal pathological injury
    Study

    mousen: not reported in source

    Diabetic nephropathy mice (high-fat diet plus streptozotocin)
    Comparator
    Untreated DN mice
    Outcome group
    Kidney function · Kidney and urinary
    Studied dose
    High doseAs given in this study (mouse).

    Source sentences

    Summary
    alleviated renal pathological injury compared to untreated DN mice.
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In mice with diabetic nephropathy, S14G-humanin inhibited tubular necroptosis, with a 52.2% reduction in p-MLKL expression.

    In plain wordsIn mice with diabetic nephropathy, S14G-humanin reduced a marker of programmed kidney cell death.

    Peptide
    Humanin
    Outcome
    DecreaseTubular necroptosis (p-MLKL expression)
    Study

    mousen: not reported in source

    Diabetic nephropathy mice (high-fat diet plus streptozotocin)
    Comparator
    Untreated DN mice
    Outcome group
    Receptor activity and mechanism · Kidney and urinary
    Studied dose
    Dose not stated

    Measurements

    p-MLKL expression reduction
    52.2%
    HNG

    Source sentences

    Summary
    Mechanistically, transcriptomics and immunoblotting revealed that HNG markedly inhibited tubular necroptosis, as evidenced by a 52.2% reduction in p-MLKL expression (P < 0.001).
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In mice with diabetic nephropathy, S14G-humanin suppressed mitochondrial BAX translocation.

    In plain wordsIn mice with diabetic nephropathy, S14G-humanin reduced movement of the BAX protein to mitochondria.

    Peptide
    Humanin
    Outcome
    DecreaseMitochondrial BAX translocation
    Study

    mousen: not reported in source

    Diabetic nephropathy mice (high-fat diet plus streptozotocin)
    Comparator
    Untreated DN mice
    Outcome group
    Mitochondrial function · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG also suppressed mitochondrial BAX translocation, reduced mitochondrial ROS production, and blocked mitochondrial Z-DNA leakage, which consequently abrogated ZBP-1 upregulation and ZBP1-mediated necroptosis.
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In mice with diabetic nephropathy, S14G-humanin reduced mitochondrial ROS production.

    In plain wordsIn mice with diabetic nephropathy, S14G-humanin lowered reactive oxygen species made by mitochondria.

    Peptide
    Humanin
    Outcome
    DecreaseMitochondrial ROS production
    Study

    mousen: not reported in source

    Diabetic nephropathy mice (high-fat diet plus streptozotocin)
    Comparator
    Untreated DN mice
    Outcome group
    Mitochondrial function · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG also suppressed mitochondrial BAX translocation, reduced mitochondrial ROS production, and blocked mitochondrial Z-DNA leakage, which consequently abrogated ZBP-1 upregulation and ZBP1-mediated necroptosis.
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In mice with diabetic nephropathy, S14G-humanin blocked mitochondrial Z-DNA leakage.

    In plain wordsIn mice with diabetic nephropathy, S14G-humanin blocked leakage of Z-DNA from mitochondria.

    Peptide
    Humanin
    Outcome
    DecreaseMitochondrial Z-DNA leakage
    Study

    mousen: not reported in source

    Diabetic nephropathy mice (high-fat diet plus streptozotocin)
    Comparator
    Untreated DN mice
    Outcome group
    Mitochondrial function · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG also suppressed mitochondrial BAX translocation, reduced mitochondrial ROS production, and blocked mitochondrial Z-DNA leakage, which consequently abrogated ZBP-1 upregulation and ZBP1-mediated necroptosis.
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In mice with diabetic nephropathy, S14G-humanin abrogated ZBP-1 upregulation and ZBP1-mediated necroptosis.

    In plain wordsIn mice with diabetic nephropathy, S14G-humanin prevented a rise in ZBP-1 and the cell death it drives.

    Peptide
    Humanin
    Outcome
    DecreaseZBP1 upregulation and ZBP1-mediated necroptosis
    Study

    mousen: not reported in source

    Diabetic nephropathy mice (high-fat diet plus streptozotocin)
    Comparator
    Untreated DN mice
    Outcome group
    Receptor activity and mechanism · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG also suppressed mitochondrial BAX translocation, reduced mitochondrial ROS production, and blocked mitochondrial Z-DNA leakage, which consequently abrogated ZBP-1 upregulation and ZBP1-mediated necroptosis.
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  9. 09AnimalAnimal study (in vivo)Efficacy outcome

    In db/db diabetic mice, the protective effects of S14G-humanin on the kidney were corroborated.

    In plain wordsIn db/db diabetic mice, the kidney-protective effects of S14G-humanin seen in the first model were confirmed.

    Peptide
    Humanin
    Outcome
    Not applicableRenal protection in db/db diabetic mice
    Study

    mousen: not reported in source

    db/db diabetic mice
    Outcome group
    Kidney function · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    These protective effects were corroborated in db/db diabetic mice and HG + PA-challenged HK-2 tubular cells.
    1 context sentence
    Summary · context
    DN was induced in C57BL/6 mice via high-fat diet and streptozotocin (STZ), with or without HNG treatment.
  10. 10LabIn vitro (cells)Efficacy outcome

    In HK-2 tubular cells challenged with high glucose and palmitate, the protective effects of S14G-humanin were corroborated.

    In plain wordsIn kidney tubular cells under high glucose and palmitate stress, S14G-humanin protective effects seen in mice were confirmed.

    Peptide
    Humanin
    Outcome
    Not applicableProtection of HG + PA-challenged HK-2 tubular cells
    Study

    humann: not reported in source

    HK-2 tubular cells challenged with high glucose and palmitate
    Outcome group
    Kidney function · Kidney and urinary
    Studied dose
    Dose not stated

    Source sentences

    Summary
    These protective effects were corroborated in db/db diabetic mice and HG + PA-challenged HK-2 tubular cells.
    1 context sentence
    Summary · context
    key findings were validated in db/db mice and high glucose/palmitate (HG + PA)-treated HK-2 cells.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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