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Study · Ex vivo tissue · rat · 2026

8 results

Mitochondrial-derived peptide Humanin protects granulosa cells under oxidative conditions.

Conte JG, Reynoso S, Gosso MS, Sánchez Rojas IA, Romero AC, Magnani ND, Jaita G

Reproduction (Cambridge, England)1 Jul 2026

PMID42397223 (opens in a new tab)DOI10.1093/reprod/xaag082 (opens in a new tab)PepBankPUB-42397223
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In cultured rat ovaries under oxidative conditions, HN protected granulosa cells of antral follicles.

    In plain wordsIn cultured rat ovaries under oxidative stress, humanin protected granulosa cells of antral follicles.

    Peptide
    Humanin
    Outcome
    IncreaseProtection of granulosa cells of antral follicles
    Study

    ratn: not reported in source

    In vitro rat ovary culture
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Consistently, in rat ovaries, HN protected granulosa cells of antral follicles under oxidative conditions.
    1 context sentence
    Summary · context
    The present study aimed to evaluate the cytoprotective effects of HN under oxidative conditions in granulosa cells, using a human granulosa cell line (KGN) and an in-vitro rat ovary culture to assess its action within the ovarian microenvironment.
  2. 02LabIn vitro (cells)Mechanism

    In KGN cells under oxidative insult, exogenous HN significantly increased catalase activity.

    In plain wordsIn human granulosa cells under oxidative stress, added humanin raised catalase activity.

    Peptide
    Humanin
    Outcome
    IncreaseCatalase activity
    Study

    humann: not reported in source

    KGN human granulosa cell line
    Comparator
    H2O2 without HN
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    exogenous HN enhanced cellular antioxidant capacity by significantly increasing catalase activity levels
  3. 03LabIn vitro (cells)Mechanism

    In KGN cells under oxidative insult, exogenous HN did not modify superoxide dismutase expression or activity, or redox status.

    In plain wordsIn human granulosa cells under oxidative stress, added humanin did not change superoxide dismutase or redox status.

    Peptide
    Humanin
    Outcome
    No significant changeSuperoxide dismutase expression or activity and redox status
    Study

    humann: not reported in source

    KGN human granulosa cell line
    Comparator
    H2O2 without HN
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    without modifying superoxide dismutase expression or activity, or redox status in KGN cells
  4. 04LabIn vitro (cells)Efficacy outcome

    In KGN cells, HN significantly decreased H2O2-induced apoptosis in a TUNEL assay.

    In plain wordsIn human granulosa cells, humanin reduced cell death caused by hydrogen peroxide.

    Peptide
    Humanin
    Outcome
    DecreaseH2O2-induced granulosa cell apoptosis
    Study

    humann: not reported in source

    KGN human granulosa cell line
    Comparator
    H2O2 without HN
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Importantly, HN significantly decreased H2O2-induced granulosa cell apoptosis in KGN cells, as determined by the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.
  5. 05LabIn vitro (cells)Mechanism

    In KGN cells under oxidative insult, HN reduced expression of BAX.

    In plain wordsIn human granulosa cells under oxidative stress, humanin lowered the activity of the cell-death gene BAX.

    Peptide
    Humanin
    Outcome
    DecreaseBAX expression
    Study

    humann: not reported in source

    KGN human granulosa cell line
    Comparator
    H2O2 without HN
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    This protective effect was associated with modulation of key apoptosis-related genes, including reduced expression of BAX and the caspase-3 precursor (CASP3), while BCL2 expression remained unchanged.
  6. 06LabIn vitro (cells)Mechanism

    In KGN cells under oxidative insult, HN reduced expression of the caspase-3 precursor.

    In plain wordsIn human granulosa cells under oxidative stress, humanin lowered the activity of the caspase-3 precursor gene.

    Peptide
    Humanin
    Outcome
    DecreaseCaspase-3 precursor (CASP3) expression
    Study

    humann: not reported in source

    KGN human granulosa cell line
    Comparator
    H2O2 without HN
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    This protective effect was associated with modulation of key apoptosis-related genes, including reduced expression of BAX and the caspase-3 precursor (CASP3), while BCL2 expression remained unchanged.
  7. 07LabIn vitro (cells)Mechanism

    In KGN cells under oxidative insult, BCL2 expression remained unchanged with HN.

    In plain wordsIn human granulosa cells under oxidative stress, humanin did not change the BCL2 gene.

    Peptide
    Humanin
    Outcome
    No significant changeBCL2 expression
    Study

    humann: not reported in source

    KGN human granulosa cell line
    Comparator
    H2O2 without HN
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    This protective effect was associated with modulation of key apoptosis-related genes, including reduced expression of BAX and the caspase-3 precursor (CASP3), while BCL2 expression remained unchanged.

Natural levels measured1

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 08LabEndogenous levelIn vitro (cells)Other

    In KGN granulosa cells, H2O2-induced oxidative conditions significantly increased endogenous HN mRNA expression.

    In plain wordsIn human granulosa cells, oxidative stress raised the cells' own humanin gene activity.

    Peptide
    Humanin
    Outcome
    IncreaseEndogenous HN mRNA expression under H2O2
    Study

    humann: not reported in source

    KGN human granulosa cell line
    Comparator
    Cells without H2O2
    Outcome group
    Mitochondrial function · Reproductive and sexual health

    Source sentences

    Summary
    KGN cells showed a significant increase in endogenous HN mRNA expression in response to oxidative conditions induced by H2O2.

    This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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