Kisspeptin-10 regulates glycosaminoglycan and decorin content in human cardiac fibroblast cultures.

Radwańska P, Olkiewicz M, Piera L, Drobnik J

Pharmacological reports : PR20 May 2026

PMID42159865PMCIDPMC13437659DOI10.1007/s43440-026-00870-6PepBankPUB-42159865

Summary

BackgroundCardiac fibroblasts play a key role in extracellular matrix (ECM) remodelling through the synthesis of glycosaminoglycans and proteoglycans (PGs), such as decorin; however, the effects of kisspeptin-10 (KiSS-10) on these components in the heart remain unknown. The aim of this study was to determine the influence of KiSS-10 on glycosaminoglycan and decorin content in human cardiac fibroblast cultures and identify its mechanism.MethodsThe effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor. Glycosaminoglycan levels were determined by the Farndale method, decorin secretion by enzyme-linked immunosorbent assay (ELISA), and decorin expression by quantitative polymerase chain reaction (qPCR).ResultsKiSS-10 treatment caused a dose-dependent increase in glycosaminoglycan content in both cells and medium. This stimulatory effect was abolished by blocking G-protein-coupled receptor 54 (GPR54) with peptide 234 or focal adhesion kinase (FAK) with FAK inhibitor 14 (FAKi). Combining KiSS-10 with phospholipase C inhibitor (D609) did not alter the glycosaminoglycan level compared to KiSS-10 alone. KiSS-10 increased decorin secretion but not expression. Combining peptide 234 with KiSS-10 did not alter media decorin level compared to KiSS-10 alone.ConclusionsKiSS-10 significantly elevated glycosaminoglycan and decorin accumulation in human cardiac fibroblast cultures. This regulatory effect on glycosaminoglycan level appears to be dependent on GPR54 activation and FAK signalling, but not on phospholipase C function. Hence, KiSS-10 may be involved in ECM remodelling in the heart.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In human cardiac fibroblasts, KiSS-10 caused a dose-dependent increase in glycosaminoglycan content in cells and medium.
In plain wordsIn human heart cells in the lab, KiSS-10 increased glycosaminoglycan content, and more so at higher doses.
Peptide
Kisspeptin-10
Outcome
IncreaseGlycosaminoglycan content in cells and medium
Study
humann: not reported in source
Human cardiac fibroblast cell line
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
KiSS-10 treatment caused a dose-dependent increase in glycosaminoglycan content in both cells and medium.
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor.
Show in the study text Report a problemFND-4215986501
02LabIn vitro (cells)Mechanism
In human cardiac fibroblasts, blocking GPR54 with peptide 234 abolished the KiSS-10-induced increase in glycosaminoglycan content.
In plain wordsIn human heart cells in the lab, blocking the GPR54 receptor removed the glycosaminoglycan increase caused by KiSS-10.
Peptide
Kisspeptin-10
Outcome
DecreaseGlycosaminoglycan increase with GPR54 blockade
Study
humann: not reported in source
Human cardiac fibroblast cell line
Comparator
KiSS-10 alone
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
This stimulatory effect was abolished by blocking G-protein-coupled receptor 54 (GPR54) with peptide 234
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor.
Show in the study text Report a problemFND-4215986502
03LabIn vitro (cells)Mechanism
In human cardiac fibroblasts, FAK inhibitor 14 abolished the KiSS-10-induced increase in glycosaminoglycan content.
In plain wordsIn human heart cells in the lab, blocking the FAK enzyme removed the glycosaminoglycan increase caused by KiSS-10.
Peptide
Kisspeptin-10
Outcome
DecreaseGlycosaminoglycan increase with FAK inhibition
Study
humann: not reported in source
Human cardiac fibroblast cell line
Comparator
KiSS-10 alone
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
This stimulatory effect was abolished by blocking G-protein-coupled receptor 54 (GPR54) with peptide 234 or focal adhesion kinase (FAK) with FAK inhibitor 14 (FAKi)
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor.
Show in the study text Report a problemFND-4215986503
04LabIn vitro (cells)Mechanism
In human cardiac fibroblasts, adding the phospholipase C inhibitor D609 to KiSS-10 did not change glycosaminoglycan level versus KiSS-10 alone.
In plain wordsIn human heart cells in the lab, blocking phospholipase C did not change the KiSS-10 effect on glycosaminoglycan.
Peptide
Kisspeptin-10
Outcome
No significant changeGlycosaminoglycan level with phospholipase C inhibitor
Study
humann: not reported in source
Human cardiac fibroblast cell line
Comparator
KiSS-10 alone
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Combining KiSS-10 with phospholipase C inhibitor (D609) did not alter the glycosaminoglycan level compared to KiSS-10 alone.
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor.
Show in the study text Report a problemFND-4215986504
05LabIn vitro (cells)Efficacy outcome
In human cardiac fibroblasts, KiSS-10 increased decorin secretion.
In plain wordsIn human heart cells in the lab, KiSS-10 increased release of the matrix protein decorin.
Peptide
Kisspeptin-10
Outcome
IncreaseDecorin secretion
Study
humann: not reported in source
Human cardiac fibroblast cell line
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
KiSS-10 increased decorin secretion
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor.
Show in the study text Report a problemFND-4215986505
06LabIn vitro (cells)Mechanism
In human cardiac fibroblasts, KiSS-10 did not increase decorin gene expression.
In plain wordsIn human heart cells in the lab, KiSS-10 did not raise decorin gene activity.
Peptide
Kisspeptin-10
Outcome
No significant changeDecorin expression
Study
humann: not reported in source
Human cardiac fibroblast cell line
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
but not expression.
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor.
Show in the study text Report a problemFND-4215986506
07LabIn vitro (cells)Mechanism
In human cardiac fibroblasts, adding peptide 234 to KiSS-10 did not change media decorin level versus KiSS-10 alone.
In plain wordsIn human heart cells in the lab, blocking the GPR54 receptor did not change decorin release caused by KiSS-10.
Peptide
Kisspeptin-10
Outcome
No significant changeMedia decorin with GPR54 blockade
Study
humann: not reported in source
Human cardiac fibroblast cell line
Comparator
KiSS-10 alone
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
Combining peptide 234 with KiSS-10 did not alter media decorin level compared to KiSS-10 alone.
1 context sentence(design, population, supporting numbers)
Summary · context
The effects of KiSS-10 on glycosaminoglycans and decorin were assessed in human cardiac fibroblast cell line, with or without signalling inhibitor.
Show in the study text Report a problemFND-4215986507

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

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