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Study · In vitro (cells) · 2026

6 results

Humanin improved the rotenone-induced reactive oxygen species formation in PC12 cells by modulating the SIRT3/Nrf2/HO-1 signaling pathway.

Shan Y, Liu X, Ge W, Zhang Q, Yan S

Toxicology and industrial health27 Apr 2026

PMID42041115 (opens in a new tab)DOI10.1177/07482337261445494 (opens in a new tab)PepBankPUB-42041115
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In PC12 cells exposed to rotenone, HN pretreatment significantly increased cell survival.

    In plain wordsIn cells exposed to rotenone, humanin pretreatment increased cell survival.

    Peptide
    Humanin
    Outcome
    IncreasePC12 cell survival
    Study

    n: not reported in source

    PC12 cells with rotenone-induced neurotoxicity
    Comparator
    Rotenone without HN
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HN pretreatment significantly increased PC12 cell survival, reduced ROS formation, and increased the NAD+/NADH ratio.
    1 context sentence
    Summary · context
    PC12 cells were preincubated with HN, which has a protective effect.
  2. 02LabIn vitro (cells)Activity measurement

    In PC12 cells exposed to rotenone, HN pretreatment reduced ROS formation.

    In plain wordsIn cells exposed to rotenone, humanin pretreatment reduced the formation of reactive oxygen species.

    Peptide
    Humanin
    Outcome
    DecreaseReactive oxygen species formation
    Study

    n: not reported in source

    PC12 cells with rotenone-induced neurotoxicity
    Comparator
    Rotenone without HN
    Outcome group
    Oxidative stress · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HN pretreatment significantly increased PC12 cell survival, reduced ROS formation, and increased the NAD+/NADH ratio.
    1 context sentence
    Summary · context
    PC12 cells were preincubated with HN, which has a protective effect.
  3. 03LabIn vitro (cells)Mechanism

    In PC12 cells exposed to rotenone, HN pretreatment increased the NAD+/NADH ratio.

    In plain wordsIn cells exposed to rotenone, humanin pretreatment raised the NAD+/NADH ratio.

    Peptide
    Humanin
    Outcome
    IncreaseNAD+/NADH ratio
    Study

    n: not reported in source

    PC12 cells with rotenone-induced neurotoxicity
    Comparator
    Rotenone without HN
    Outcome group
    Mitochondrial function · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HN pretreatment significantly increased PC12 cell survival, reduced ROS formation, and increased the NAD+/NADH ratio.
    1 context sentence
    Summary · context
    PC12 cells were preincubated with HN, which has a protective effect.
  4. 04LabIn vitro (cells)Mechanism

    In PC12 cells under rotenone exposure, HN increased the expression of SIRT3, Nrf2, HO-1 and NQO1 proteins.

    In plain wordsIn cells under rotenone exposure, humanin raised the levels of the SIRT3, Nrf2, HO-1 and NQO1 proteins.

    Peptide
    Humanin
    Outcome
    IncreaseSIRT3, Nrf2, HO-1 and NQO1 protein expression
    Study

    n: not reported in source

    PC12 cells with rotenone-induced neurotoxicity
    Comparator
    Rotenone without HN
    Outcome group
    Oxidative stress · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It also increased the expression of SIRT3, Nrf2, HO-1, and NQO1 proteins and decreased the expression of Ac-SOD protein under rotenone exposure.
    1 context sentence
    Summary · context
    PC12 cells were preincubated with HN, which has a protective effect.
  5. 05LabIn vitro (cells)Mechanism

    In PC12 cells under rotenone exposure, HN decreased the expression of Ac-SOD protein.

    In plain wordsIn cells under rotenone exposure, humanin lowered the level of Ac-SOD protein.

    Peptide
    Humanin
    Outcome
    DecreaseAc-SOD protein expression
    Study

    n: not reported in source

    PC12 cells with rotenone-induced neurotoxicity
    Comparator
    Rotenone without HN
    Outcome group
    Oxidative stress · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It also increased the expression of SIRT3, Nrf2, HO-1, and NQO1 proteins and decreased the expression of Ac-SOD protein under rotenone exposure.
    1 context sentence
    Summary · context
    PC12 cells were preincubated with HN, which has a protective effect.
  6. 06LabIn vitro (cells)Mechanism

    In PC12 cells, HN activated the Nrf2/HO-1 signaling pathway, which depends on HN-mediated SIRT3 activation.

    In plain wordsIn cells, humanin switched on the Nrf2/HO-1 pathway, and this depended on SIRT3 activation by humanin.

    Peptide
    Humanin
    Outcome
    IncreaseNrf2/HO-1 pathway activation via SIRT3
    Study

    n: not reported in source

    PC12 cells with rotenone-induced neurotoxicity
    Comparator
    Rotenone without HN
    Outcome group
    Oxidative stress · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    it also activated the Nrf2/HO-1 signaling pathway, which depends on HN-mediated SIRT3 activation
    1 context sentence
    Summary · context
    PC12 cells were preincubated with HN, which has a protective effect.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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