Relationship between kisspeptin-10, neurokinin B and dynorphin A in the course of normal and delayed puberty in ewes.

Szysiak N, Kosior-Korzecka U, Greguła-Kania M, Patkowski K, Fila M, Junkuszew A

Journal of veterinary research2 Mar 2026

PMID41953732PMCIDPMC13054753DOI10.2478/jvetres-2026-0010PepBankPUB-41953732

Summary

IntroductionKisspeptin (KiSS), neurokinin B (NKB) and dynorphin A (Dyn A) participate in the neuroendocrine regulation of reproductive system development and functioning; however, their roles in the onset of sexual maturity and pathomechanism of delayed puberty have not yet been fully elucidated. The aim of the study was to determine changes in the kisspeptin-10 (KiSS-10), NKB and Dyn A concentration in blood plasma collected from ewe lambs during puberty in relation to the first ovulation time, and to examine the relationship between these neuropeptides.Material and methodsTwenty-four ewe lambs were divided into two groups: from single (S) pregnancies and from twin (T) pregnancies. To determine the KiSS-10, NKB and Dyn A concentration with ELISA, blood was collected from all sheep at 4, 5, 6, 7, 8, 9 and 10 months of age. Singletons and twins had their first ovulations at 8 and 10 months of age, respectively.ResultsChanges in the KiSS-10 and NKB concentrations were positively correlated with age in both S (r = 0.86) and T ewes (r = 0.89). A negative correlation was found between KiSS-10 and Dyn A (r = -0.55 and -0.98, for S and T, respectively) and between NKB and Dyn A (r = -0.89 and -0.94, for S and T, respectively).ConclusionThe initiation of ovarian activity is associated with concurrent increases in plasma KiSS-10 and NKB concentrations with age and reduced Dyn A concentrations in both groups. Our results show that these neuropeptides may regulate the timing of reproductive activity onset in sheep.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In ewe lambs, plasma KiSS-10 concentration was positively correlated with age during puberty in singletons and twins.
In plain wordsIn young ewes, the body's own kisspeptin‑10 level rose with age as puberty approached.
Peptide
Kisspeptin-10
Outcome
IncreasePlasma KiSS-10 correlation with age
Study
sheepn = 24
Ewe lambs, singleton and twin
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Correlation coefficient r
0.86 r
singletons
Correlation coefficient r
0.89 r
twins
Source sentences
Summary
Changes in the KiSS-10 and NKB concentrations were positively correlated with age in both S (r = 0.86) and T ewes (r = 0.89).
2 context sentences(design, population, supporting numbers)
Summary · context
Twenty-four ewe lambs were divided into two groups: from single (S) pregnancies and from twin (T) pregnancies.
Summary · context
To determine the KiSS-10, NKB and Dyn A concentration with ELISA, blood was collected from all sheep at 4, 5, 6, 7, 8, 9 and 10 months of age.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-4195373201
02AnimalEndogenous levelAnimal study (in vivo)Other
In ewe lambs, plasma KiSS-10 concentration was negatively correlated with dynorphin A concentration in singletons and twins.
In plain wordsIn young ewes, the body's own kisspeptin‑10 level was lower when dynorphin A was higher.
Peptide
Kisspeptin-10
Outcome
DecreasePlasma KiSS-10 correlation with dynorphin A
Study
sheepn = 24
Ewe lambs, singleton and twin
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Correlation coefficient r
−0.55 r
singletons
Correlation coefficient r
−0.98 r
twins
Source sentences
Summary
A negative correlation was found between KiSS-10 and Dyn A (r = -0.55 and -0.98, for S and T, respectively) and between NKB and Dyn A (r = -0.89 and -0.94, for S and T, respectively).
2 context sentences(design, population, supporting numbers)
Summary · context
Twenty-four ewe lambs were divided into two groups: from single (S) pregnancies and from twin (T) pregnancies.
Summary · context
To determine the KiSS-10, NKB and Dyn A concentration with ELISA, blood was collected from all sheep at 4, 5, 6, 7, 8, 9 and 10 months of age.
This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-4195373202

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
10 Oct 2026, 09:43 UTC
Study results updated
Version 1
9 Oct 2026, 03:04 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Keywords

PepBank is a research reference. Talk to a doctor before you use any peptide.