Nicotinamide Mononucleotide Improves High-Fat Diet-Induced Myocardial Damage of Aging Mice through the Sirt3/PINK1/Parkin Signaling Pathway.

Wen X, Chen YN, Han YC, Wang JP, Dong WH, Wang ZP, Wang T, Xiong ZE

The Journal of nutrition26 Feb 2026

PMID41763569DOI10.1016/j.tjnut.2026.101435PepBankPUB-41763569

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study24

22 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In aging mice, NMN reduced heart index compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart index compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseHeart index
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Heart function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Compared with the HFD group, NMN treatment significantly reduced the heart index, ameliorated myocardial fibrosis
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356901
02AnimalAnimal study (in vivo)Efficacy outcome
In aging mice, NMN ameliorated myocardial fibrosis compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart scarring compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseMyocardial fibrosis
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Heart function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Compared with the HFD group, NMN treatment significantly reduced the heart index, ameliorated myocardial fibrosis
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356902
03AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac Serpine1 expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart Serpine1 expression compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseSerpine1 expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Aging markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
and decreased the expression concentrations of senescence-associated secretory phenotype markers (Serpine1, MMP3, CXCL-1, CXCL-10, and P16; P < 0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356903
04AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac MMP3 expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart MMP3 expression compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseMMP3 expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Aging markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
and decreased the expression concentrations of senescence-associated secretory phenotype markers (Serpine1, MMP3, CXCL-1, CXCL-10, and P16; P < 0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356904
05AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac CXCL-1 expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart CXCL-1 expression compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseCXCL-1 expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Aging markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
and decreased the expression concentrations of senescence-associated secretory phenotype markers (Serpine1, MMP3, CXCL-1, CXCL-10, and P16; P < 0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356905
06AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac CXCL-10 expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart CXCL-10 expression compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseCXCL-10 expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Aging markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
and decreased the expression concentrations of senescence-associated secretory phenotype markers (Serpine1, MMP3, CXCL-1, CXCL-10, and P16; P < 0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356906
07AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac P16 expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart P16 expression compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseP16 expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Aging markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
and decreased the expression concentrations of senescence-associated secretory phenotype markers (Serpine1, MMP3, CXCL-1, CXCL-10, and P16; P < 0.05)
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356907
08AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac P21 expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart P21 expression compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseP21 expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Aging markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
senescence markers (P21 and β-galactosidase (β-gal); P < 0.01)
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356908
09AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac β-galactosidase expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart β-galactosidase expression compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Decreaseβ-galactosidase expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Aging markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
senescence markers (P21 and β-galactosidase (β-gal); P < 0.01)
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356909
10AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac IL-1β expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart IL-1β expression compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseIL-1β expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Inflammation markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
proinflammatory cytokines [interleukin (IL)-1β and tumor necrosis factor α; P < 0.05]
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356910
11AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac tumor necrosis factor α expression compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart tumor necrosis factor α compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseTNF-α expression
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Inflammation markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
proinflammatory cytokines [interleukin (IL)-1β and tumor necrosis factor α; P < 0.05]
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356911
12AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased the cardiac Bax/Bcl-2 ratio compared with the HFD group.
In plain wordsIn aging mice, NMN reduced the heart Bax/Bcl-2 ratio compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseBax/Bcl-2 ratio
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Heart function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
and apoptotic indicators (Bax/Bcl-2 ratio and cleaved caspase-3; P < 0.01).
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356912
13AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac cleaved caspase-3 compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart cleaved caspase-3 compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseCleaved caspase-3
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Heart function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
and apoptotic indicators (Bax/Bcl-2 ratio and cleaved caspase-3; P < 0.01).
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356913
14AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN increased cardiac IL-10 concentration compared with the HFD group.
In plain wordsIn aging mice, NMN increased heart IL-10 compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseIL-10 concentration
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Inflammation markers · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Conversely, NMN treatment upregulated the concentrations of anti-inflammatory factor IL-10 (P < 0.01) in cardiac tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356914
15AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN increased cardiac Sirt3 protein concentration compared with the HFD group.
In plain wordsIn aging mice, NMN increased heart Sirt3 protein compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseSirt3 concentration
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Mitochondrial function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Furthermore, NMN treatment increased protein concentrations of Sirt3, PINK1, and Parkin (P < 0.01), and enhanced autophagy-lysosomal markers, including LC3-II/LC3-I ratio and transcription factor EB (TFEB) (P < 0.05), and decreased p62 concentrations (P < 0.01) in cardiac tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356915
16AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN increased cardiac PINK1 protein concentration compared with the HFD group.
In plain wordsIn aging mice, NMN increased heart PINK1 protein compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreasePINK1 concentration
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Mitochondrial function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Furthermore, NMN treatment increased protein concentrations of Sirt3, PINK1, and Parkin (P < 0.01), and enhanced autophagy-lysosomal markers, including LC3-II/LC3-I ratio and transcription factor EB (TFEB) (P < 0.05), and decreased p62 concentrations (P < 0.01) in cardiac tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356916
17AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN increased cardiac Parkin protein concentration compared with the HFD group.
In plain wordsIn aging mice, NMN increased heart Parkin protein compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseParkin concentration
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Mitochondrial function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Furthermore, NMN treatment increased protein concentrations of Sirt3, PINK1, and Parkin (P < 0.01), and enhanced autophagy-lysosomal markers, including LC3-II/LC3-I ratio and transcription factor EB (TFEB) (P < 0.05), and decreased p62 concentrations (P < 0.01) in cardiac tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356917
18AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN increased the cardiac LC3-II/LC3-I ratio compared with the HFD group.
In plain wordsIn aging mice, NMN increased the heart LC3-II/LC3-I ratio compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseLC3-II/LC3-I ratio
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Mitochondrial function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Furthermore, NMN treatment increased protein concentrations of Sirt3, PINK1, and Parkin (P < 0.01), and enhanced autophagy-lysosomal markers, including LC3-II/LC3-I ratio and transcription factor EB (TFEB) (P < 0.05), and decreased p62 concentrations (P < 0.01) in cardiac tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356918
19AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN increased cardiac TFEB concentration compared with the HFD group.
In plain wordsIn aging mice, NMN increased heart TFEB compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseTFEB concentration
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Mitochondrial function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Furthermore, NMN treatment increased protein concentrations of Sirt3, PINK1, and Parkin (P < 0.01), and enhanced autophagy-lysosomal markers, including LC3-II/LC3-I ratio and transcription factor EB (TFEB) (P < 0.05), and decreased p62 concentrations (P < 0.01) in cardiac tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356919
20AnimalAnimal study (in vivo)Mechanism
In aging mice, NMN decreased cardiac p62 concentration compared with the HFD group.
In plain wordsIn aging mice, NMN reduced heart p62 compared with the high-fat-diet group.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
Decreasep62 concentration
Study
mousen: not reported in source
aging male C57BL/6J mice receiving high-fat diet
Comparator
HFD group
Outcome group
Mitochondrial function · Cardiovascular
Studied dose
400 mg/kg · drinking water · 7 moAs given in this study (mouse).
Source sentences
Summary
Furthermore, NMN treatment increased protein concentrations of Sirt3, PINK1, and Parkin (P < 0.01), and enhanced autophagy-lysosomal markers, including LC3-II/LC3-I ratio and transcription factor EB (TFEB) (P < 0.05), and decreased p62 concentrations (P < 0.01) in cardiac tissue.
1 context sentence(design, population, supporting numbers)
Summary · context
Male C57BL/6J mice (14-month-old) were assigned to 3 groups: normal diet, HFD, and HFD + NMN. NMN was added to their drinking water at a dose of 400 mg/kg for 7 mo.
Report a problemFND-4176356920
21LabIn vitro (cells)Efficacy outcome
In H9c2 cardiomyocytes, NMN attenuated palmitic-acid-induced cytotoxicity.
In plain wordsIn heart cells, NMN reduced toxicity compared with palmitic acid exposure.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseCytotoxicity
Study
n: not reported in source
palmitic-acid-exposed H9c2 cardiomyocytes
Comparator
palmitic acid exposure
Outcome group
Heart function · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
In H9c2 cardiomyocytes, NMN treatment significantly attenuated PA-induced cytotoxicity (P < 0.01) and enhanced PA-induced impairment of autophagy flux.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro, H9c2 cardiomyocytes were exposed to palmitic acid (PA) to establish a lipotoxicity model, and the effects of NMN on cell viability and autophagy flux were assessed by the Methyl Thiazol Tetrazolium (MTT) assay and mRFP-GFP-LC3 adenoviral transfection.
Report a problemFND-4176356921
22LabIn vitro (cells)Mechanism
In H9c2 cardiomyocytes, NMN reportedly enhanced palmitic-acid-induced impairment of autophagy flux, despite the abstract's opposing protective interpretation.
In plain wordsIn heart cells, the result sentence reports greater autophagy impairment with NMN than with palmitic acid exposure.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
IncreaseAutophagy flux impairment
Study
n: not reported in source
palmitic-acid-exposed H9c2 cardiomyocytes
Comparator
palmitic acid exposure
Outcome group
Mitochondrial function · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
In H9c2 cardiomyocytes, NMN treatment significantly attenuated PA-induced cytotoxicity (P < 0.01) and enhanced PA-induced impairment of autophagy flux.
1 context sentence(design, population, supporting numbers)
Summary · context
In vitro, H9c2 cardiomyocytes were exposed to palmitic acid (PA) to establish a lipotoxicity model, and the effects of NMN on cell viability and autophagy flux were assessed by the Methyl Thiazol Tetrazolium (MTT) assay and mRFP-GFP-LC3 adenoviral transfection.
Report a problemFND-4176356922

Studies that did not test the peptide alone2

In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NMN (nicotinamide mononucleotide).

23LabNot a test of NMN (nicotinamide mononucleotide) aloneIn vitro (cells)Mechanism
In H9c2 cardiomyocytes, cotreatment with the Sirt3 inhibitor 3-TYP abolished NMN-associated cytoprotection.
In plain wordsIn heart cells, adding 3-TYP abolished cell protection compared with NMN alone.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
DecreaseSirt3 dependence of cytoprotection
Study
n: not reported in source
palmitic-acid-exposed H9c2 cardiomyocytes
Comparator
NMN without 3-TYP
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Notably, these protective effects were abolished by cotreatment with 3-TYP, a selective Sirt3 inhibitor.
2 context sentences(design, population, supporting numbers)
Summary · context
In H9c2 cardiomyocytes, NMN treatment significantly attenuated PA-induced cytotoxicity (P < 0.01) and enhanced PA-induced impairment of autophagy flux.
Summary · context
In vitro, H9c2 cardiomyocytes were exposed to palmitic acid (PA) to establish a lipotoxicity model, and the effects of NMN on cell viability and autophagy flux were assessed by the Methyl Thiazol Tetrazolium (MTT) assay and mRFP-GFP-LC3 adenoviral transfection.
In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).
Report a problemFND-4176356923
24LabNot a test of NMN (nicotinamide mononucleotide) aloneIn vitro (cells)Mechanism
In H9c2 cardiomyocytes, 3-TYP abolished the autophagy-related effect described as protective for NMN.
In plain wordsIn heart cells, adding 3-TYP abolished the reported autophagy-related protection compared with NMN alone.
Peptide
NMN (nicotinamide mononucleotide)
Outcome
MixedSirt3 dependence of autophagy effect
Study
n: not reported in source
palmitic-acid-exposed H9c2 cardiomyocytes
Comparator
NMN without 3-TYP
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Notably, these protective effects were abolished by cotreatment with 3-TYP, a selective Sirt3 inhibitor.
2 context sentences(design, population, supporting numbers)
Summary · context
In H9c2 cardiomyocytes, NMN treatment significantly attenuated PA-induced cytotoxicity (P < 0.01) and enhanced PA-induced impairment of autophagy flux.
Summary · context
In vitro, H9c2 cardiomyocytes were exposed to palmitic acid (PA) to establish a lipotoxicity model, and the effects of NMN on cell viability and autophagy flux were assessed by the Methyl Thiazol Tetrazolium (MTT) assay and mRFP-GFP-LC3 adenoviral transfection.
In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).
Report a problemFND-4176356924

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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