Engineered Tandem Thymosin Peptide Promotes Corneal Wound Healing.

Nguyen J, Verma S, Vuong VT, Queener H, Coulson-Thomas VJ, Gesteira TF

Investigative ophthalmology & visual science1 Nov 2025

PMID41235866PMCIDPMC12636994DOI10.1167/iovs.66.14.31PepBankPUB-41235866

Read at source PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

01AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In a mouse alkali-induced corneal injury model, tTB4 promoted corneal wound healing with greater efficacy than TB4.
In plain wordsIn mice with a chemical eye burn, the engineered double thymosin beta‑4 helped the cornea heal better than TB4 did.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseCorneal wound healing
Study
mousen: not reported in source
murine alkali-induced corneal injury
Comparator
TB4
Outcome group
Wound healing · Eye
Source sentences
Summary
In vivo, tTB4 promoted corneal wound healing and reduced scarring with greater efficacy than TB4.
2 context sentences(design, population, supporting numbers)
Summary · context
Binding activity was investigated using structural, in vitro, and in vivo studies. tTB4 and TB4 effects on cell viability and migration were assessed in human telomerase-immortalized corneal epithelial (hTCEpi) cells.
Summary · context
Their efficacy in promoting corneal wound healing was tested in a murine alkali-induced corneal injury model.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-4123586604
02AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Efficacy outcome
In a mouse alkali-induced corneal injury model, tTB4 reduced scarring with greater efficacy than TB4.
In plain wordsIn mice with a chemical eye burn, the engineered double thymosin beta‑4 reduced scarring more than TB4 did.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseCorneal scarring
Study
mousen: not reported in source
murine alkali-induced corneal injury
Comparator
TB4
Outcome group
Wound healing · Eye
Source sentences
Summary
In vivo, tTB4 promoted corneal wound healing and reduced scarring with greater efficacy than TB4.
2 context sentences(design, population, supporting numbers)
Summary · context
Binding activity was investigated using structural, in vitro, and in vivo studies. tTB4 and TB4 effects on cell viability and migration were assessed in human telomerase-immortalized corneal epithelial (hTCEpi) cells.
Summary · context
Their efficacy in promoting corneal wound healing was tested in a murine alkali-induced corneal injury model.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-4123586605
03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome
In human corneal epithelial (hTCEpi) cells, tTB4 increased cell viability compared with TB4.
In plain wordsIn cells, the engineered double thymosin beta‑4 kept more corneal cells alive than TB4 did.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseCorneal epithelial cell viability
Study
humann: not reported in source
hTCEpi corneal epithelial cells
Comparator
TB4
Outcome group
Wound healing · Eye
Source sentences
Summary
In vitro, tTB4 promoted increased hTCEpi viability and migration compared to TB4.
2 context sentences(design, population, supporting numbers)
Summary · context
Binding activity was investigated using structural, in vitro, and in vivo studies. tTB4 and TB4 effects on cell viability and migration were assessed in human telomerase-immortalized corneal epithelial (hTCEpi) cells.
Summary · context
Their efficacy in promoting corneal wound healing was tested in a murine alkali-induced corneal injury model.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-4123586602
04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome
In human corneal epithelial (hTCEpi) cells, tTB4 increased cell migration compared with TB4.
In plain wordsIn cells, the engineered double thymosin beta‑4 made corneal cells move more than TB4 did.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseCorneal epithelial cell migration
Study
humann: not reported in source
hTCEpi corneal epithelial cells
Comparator
TB4
Outcome group
Wound healing · Eye
Source sentences
Summary
In vitro, tTB4 promoted increased hTCEpi viability and migration compared to TB4.
2 context sentences(design, population, supporting numbers)
Summary · context
Binding activity was investigated using structural, in vitro, and in vivo studies. tTB4 and TB4 effects on cell viability and migration were assessed in human telomerase-immortalized corneal epithelial (hTCEpi) cells.
Summary · context
Their efficacy in promoting corneal wound healing was tested in a murine alkali-induced corneal injury model.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-4123586603
05Computer modelNot a test of Thymosin beta-4 (human) aloneIn silicoMechanism
Structural modeling indicated that tandem thymosin beta‑4 (tTB4) has a mechanistic advantage over TB4 in enhancing actin polymerization, because it can bind two G-actin molecules at once.
In plain wordsA computer model suggests the engineered double thymosin beta‑4 may help build actin filaments better than TB4.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseActin polymerization mechanism (structural modeling)
Study
n: not reported in source
structural model
Comparator
TB4
Outcome group
Receptor activity and mechanism · Eye
Source sentences
Summary
Structural modeling revealed tTB4 has a mechanistic advantage in enhancing actin polymerization over TB4, as it can simultaneously bind and sequester two G-actin molecules, facilitating filament formation.
2 context sentences(design, population, supporting numbers)
Summary · context
Binding activity was investigated using structural, in vitro, and in vivo studies. tTB4 and TB4 effects on cell viability and migration were assessed in human telomerase-immortalized corneal epithelial (hTCEpi) cells.
Summary · context
Their efficacy in promoting corneal wound healing was tested in a murine alkali-induced corneal injury model.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-4123586601

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

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