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Study · Animal study (in vivo) · mouse · 2025

6 results

Systemic nicotinamide mononucleotide administration to mitigate post-cardiac arrest brain injury in mice.

Kaito D, Tamura T, Suzuki S, Onishi R, Tanaka KF, Yoshino J, Matsuoka T, Maeshima K, Sasaki J, Homma K

PloS one21 Oct 2025

PMID41118347 (opens in a new tab)PMCIDPMC12539731 (opens in a new tab)DOI10.1371/journal.pone.0334608 (opens in a new tab)PepBankPUB-41118347
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Summary

Post-cardiac arrest brain injury (PCABI) is the leading cause of death and disability following cardiac arrest (CA). Nicotinamide adenine dinucleotide (NAD+) depletion after CA contributes to neuronal injury, while nicotinamide mononucleotide (NMN) replenishes NAD+ and may provide neuroprotection via sirtuin activation. This study aimed to investigate the effects of systemic NMN administration on neurological function, survival, and sirtuin-3 (SIRT3) levels in the brain post-CA. In adult male mice (C57BL/6NCrSlc, 10-15 weeks old), 10-min CA was induced by intravenous potassium chloride injection followed by cardiopulmonary resuscitation. NMN (60 mg/kg body weight) or normal saline (control) was randomly administered by intraperitoneal injection immediately after the return of spontaneous circulation (ROSC) and 24 and 48 h post-CA. Brain NAD+ and adenosine triphosphate (ATP) levels, neurological function score (NFS), survival, histological neuronal injury, and brain gene expression and protein levels were measured. Brain NAD+ levels decreased at 2 h post-ROSC and NMN significantly increased brain NAD+ and ATP levels. At 48 h post-CA, surviving mice in the NMN group exhibited significantly higher NFS (control: 8 [IQR: 4-12] vs. NMN: 12 [IQR: 9-12], p = 0.03) and less severe hippocampal neuronal injury compared with controls. Moreover, the NMN group showed significantly higher 7-day survival rate (control: 22.2% [4/18] vs. NMN: 61.1% [11/18], p = 0.03) and brain SIRT3 levels (control: 17.7 ± 3.6 vs. NMN: 34.5 ± 4.4 pg/mg protein, p = 0.01). In conclusion, systemic NMN administration after ROSC attenuates PCABI. The increased brain ATP levels and SIRT3 upregulation may suggest the usefulness of NMN for improving mitochondrial function and contributing to neuroprotection. NAD+ supplementation with NMN is a promising therapeutic approach against PCABI.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In post-cardiac arrest mice, NMN increased 7-day survival to 61.1% versus 22.2% with saline.

    In plain wordsIn mice after cardiac arrest, NMN increased survival compared with saline.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    Increase7-day survival
    Study

    mousen = 36

    post-cardiac arrest C57BL/6NCrSlc mice
    Comparator
    normal saline
    Outcome group
    Mortality · Whole body
    Studied dose
    60 mg/kg body weight · intraperitonealAs given in this study (mouse).

    Measurements

    7-day survival
    61.1%
    NMN
    7-day survival
    22.2%
    control

    Source sentences

    Summary
    Moreover, the NMN group showed significantly higher 7-day survival rate (control: 22.2% [4/18] vs. NMN: 61.1% [11/18], p = 0.03) and brain SIRT3 levels (control: 17.7 ± 3.6 vs. NMN: 34.5 ± 4.4 pg/mg protein, p = 0.01).
    2 context sentences
    Summary · context
    In adult male mice (C57BL/6NCrSlc, 10-15 weeks old), 10-min CA was induced by intravenous potassium chloride injection followed by cardiopulmonary resuscitation.
    Summary · context
    NMN (60 mg/kg body weight) or normal saline (control) was randomly administered by intraperitoneal injection immediately after the return of spontaneous circulation (ROSC) and 24 and 48 h post-CA.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In post-cardiac arrest mice, NMN increased brain NAD+ levels.

    In plain wordsIn mice after cardiac arrest, NMN increased brain NAD+ compared with saline.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseBrain NAD+
    Study

    mousen: not reported in source

    post-cardiac arrest C57BL/6NCrSlc mice
    Comparator
    normal saline
    Outcome group
    Mitochondrial function · Nervous system and cognition
    Studied dose
    60 mg/kg body weight · intraperitonealAs given in this study (mouse).

    Source sentences

    Summary
    Brain NAD+ levels decreased at 2 h post-ROSC and NMN significantly increased brain NAD+ and ATP levels.
    2 context sentences
    Summary · context
    In adult male mice (C57BL/6NCrSlc, 10-15 weeks old), 10-min CA was induced by intravenous potassium chloride injection followed by cardiopulmonary resuscitation.
    Summary · context
    NMN (60 mg/kg body weight) or normal saline (control) was randomly administered by intraperitoneal injection immediately after the return of spontaneous circulation (ROSC) and 24 and 48 h post-CA.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In post-cardiac arrest mice, NMN increased brain ATP levels.

    In plain wordsIn mice after cardiac arrest, NMN increased brain ATP compared with saline.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseBrain ATP
    Study

    mousen: not reported in source

    post-cardiac arrest C57BL/6NCrSlc mice
    Comparator
    normal saline
    Outcome group
    Mitochondrial function · Nervous system and cognition
    Studied dose
    60 mg/kg body weight · intraperitonealAs given in this study (mouse).

    Source sentences

    Summary
    Brain NAD+ levels decreased at 2 h post-ROSC and NMN significantly increased brain NAD+ and ATP levels.
    2 context sentences
    Summary · context
    In adult male mice (C57BL/6NCrSlc, 10-15 weeks old), 10-min CA was induced by intravenous potassium chloride injection followed by cardiopulmonary resuscitation.
    Summary · context
    NMN (60 mg/kg body weight) or normal saline (control) was randomly administered by intraperitoneal injection immediately after the return of spontaneous circulation (ROSC) and 24 and 48 h post-CA.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In surviving mice at 48 h after cardiac arrest, NMN increased neurological function scores versus saline.

    In plain wordsIn surviving mice after cardiac arrest, NMN improved neurological scores compared with saline.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseNeurological function score
    Study

    mousen: not reported in source

    post-cardiac arrest C57BL/6NCrSlc mice
    Comparator
    normal saline
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg body weight · intraperitonealAs given in this study (mouse).

    Measurements

    NFS
    12 score
    NMN, median at 48 h
    NFS
    8 score
    control, median at 48 h

    Source sentences

    Summary
    At 48 h post-CA, surviving mice in the NMN group exhibited significantly higher NFS (control: 8 [IQR: 4-12] vs. NMN: 12 [IQR: 9-12], p = 0.03) and less severe hippocampal neuronal injury compared with controls.
    2 context sentences
    Summary · context
    In adult male mice (C57BL/6NCrSlc, 10-15 weeks old), 10-min CA was induced by intravenous potassium chloride injection followed by cardiopulmonary resuscitation.
    Summary · context
    NMN (60 mg/kg body weight) or normal saline (control) was randomly administered by intraperitoneal injection immediately after the return of spontaneous circulation (ROSC) and 24 and 48 h post-CA.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In surviving mice at 48 h after cardiac arrest, NMN reduced hippocampal neuronal injury versus saline.

    In plain wordsIn surviving mice after cardiac arrest, NMN reduced hippocampal nerve cell injury compared with saline.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseHippocampal neuronal injury
    Study

    mousen: not reported in source

    post-cardiac arrest C57BL/6NCrSlc mice
    Comparator
    normal saline
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    60 mg/kg body weight · intraperitonealAs given in this study (mouse).

    Source sentences

    Summary
    At 48 h post-CA, surviving mice in the NMN group exhibited significantly higher NFS (control: 8 [IQR: 4-12] vs. NMN: 12 [IQR: 9-12], p = 0.03) and less severe hippocampal neuronal injury compared with controls.
    2 context sentences
    Summary · context
    In adult male mice (C57BL/6NCrSlc, 10-15 weeks old), 10-min CA was induced by intravenous potassium chloride injection followed by cardiopulmonary resuscitation.
    Summary · context
    NMN (60 mg/kg body weight) or normal saline (control) was randomly administered by intraperitoneal injection immediately after the return of spontaneous circulation (ROSC) and 24 and 48 h post-CA.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In post-cardiac arrest mice, NMN increased brain SIRT3 levels versus saline.

    In plain wordsIn mice after cardiac arrest, NMN increased brain SIRT3 compared with saline.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseBrain SIRT3
    Study

    mousen: not reported in source

    post-cardiac arrest C57BL/6NCrSlc mice
    Comparator
    normal saline
    Outcome group
    Mitochondrial function · Nervous system and cognition
    Studied dose
    60 mg/kg body weight · intraperitonealAs given in this study (mouse).

    Measurements

    SIRT3
    34.5 pg/mg protein
    NMN
    SIRT3
    17.7 pg/mg protein
    control

    Source sentences

    Summary
    Moreover, the NMN group showed significantly higher 7-day survival rate (control: 22.2% [4/18] vs. NMN: 61.1% [11/18], p = 0.03) and brain SIRT3 levels (control: 17.7 ± 3.6 vs. NMN: 34.5 ± 4.4 pg/mg protein, p = 0.01).
    2 context sentences
    Summary · context
    In adult male mice (C57BL/6NCrSlc, 10-15 weeks old), 10-min CA was induced by intravenous potassium chloride injection followed by cardiopulmonary resuscitation.
    Summary · context
    NMN (60 mg/kg body weight) or normal saline (control) was randomly administered by intraperitoneal injection immediately after the return of spontaneous circulation (ROSC) and 24 and 48 h post-CA.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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