Exercise Improves Cardiac Dysfunction in D-Galactose-Treated Rats by Regulation of IGF-1-Humanin Pathway.

Askarimoghadam H, Rostamzadeh F, Mirtajaddini Goki M, Jafari E, Yeganeh-Hajahmadi M

Journal of aging research27 Sept 2025

PMID41050739PMCIDPMC12496137DOI10.1155/jare/9949234PepBankPUB-41050739

Summary

Introduction: Humanin, a mitochondrial-derived peptide, decreases in the elderly. This study evaluated the effects of concurrent moderate-intensity endurance training (MIET) or high-intensity interval training (HIIT) with D-galactose injection on cardiac function, and the serum and heart levels of humanin and IGF-1 in Wistar male rats. Methods: Left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), ±maxdp/dt, contractility index (CI) and, Tau were monitored by PowerLab system in CTL, CTL + MIET, CTL + HIIT, D-gal, D-gal + MIET, and D-gal + HIIT groups. The histopathological score, fibrosis, and humanin and IGF-1 levels were measured with hematoxylin & eosin, Masson's trichrome, and enzyme-linked immunosorbent assay, respectively. Results: Histopathological score and heart fibrosis were reduced by HIIT and MIET in the D-gal group. LVSP, ±maxdp/dt, and CI were lower, while LVEDP and Tau were higher in the D-gal group than in the CTL group. MIET and HIIT alleviated the changes in LVSP, ±maxdp/dt, CI, LVEDP, and Tau. HIIT and MIET increased humanin levels in heart and serum of the D-gal group by modifying IGF-1 levels. Conclusion: The study suggests HIIT and MIET may improve cardiac function by regulating the IGF-1-humanin signaling pathway.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Other
In D-galactose-treated rats, HIIT and MIET increased serum Humanin levels.
In plain wordsIn rats with induced ageing, exercise raised the body's own Humanin in the blood.
Peptide
Humanin
Outcome
IncreaseSerum Humanin after exercise
Study
ratn: not reported in source
D-galactose-treated Wistar rats
Comparator
D-galactose rats without exercise
Outcome group
Aging markers · Cardiovascular
Source sentences
Summary
HIIT and MIET increased humanin levels in heart and serum of the D-gal group by modifying IGF-1 levels.
1 context sentence(design, population, supporting numbers)
Summary · context
This study evaluated the effects of concurrent moderate-intensity endurance training (MIET) or high-intensity interval training (HIIT) with D-galactose injection on cardiac function, and the serum and heart levels of humanin and IGF-1 in Wistar male rats.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-4105073901
02AnimalEndogenous levelAnimal study (in vivo)Other
In D-galactose-treated rats, HIIT and MIET increased Humanin levels in the heart.
In plain wordsIn rats with induced ageing, exercise raised the body's own Humanin in the heart.
Peptide
Humanin
Outcome
IncreaseHeart Humanin after exercise
Study
ratn: not reported in source
D-galactose-treated Wistar rats
Comparator
D-galactose rats without exercise
Outcome group
Aging markers · Cardiovascular
Source sentences
Summary
HIIT and MIET increased humanin levels in heart and serum of the D-gal group by modifying IGF-1 levels.
1 context sentence(design, population, supporting numbers)
Summary · context
This study evaluated the effects of concurrent moderate-intensity endurance training (MIET) or high-intensity interval training (HIIT) with D-galactose injection on cardiac function, and the serum and heart levels of humanin and IGF-1 in Wistar male rats.
This result measures the body's own Humanin. Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-4105073902

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

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Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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