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Study · In vitro (cells) · pig · 2025

6 results

Mitochondrial-Targeted Protective Potential of Elamipretide for the In Vitro Production of Porcine Embryos.

Nguyen ST, Otoi T, Namula Z, Widodo OS, Tharasanit T, Chatdarong K, Nakayama Y, Nagahara M, Nakai A, Hirata M, Tanihara F

Animals : an open access journal from MDPI25 Aug 2025Cited by 3 (Europe PMC)

PMID40941292 (opens in a new tab)PMCIDPMC12427237 (opens in a new tab)DOI10.3390/ani15172497 (opens in a new tab)PepBankPUB-40941292
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Summary

Mitochondrial-targeted antioxidant supplementation is promising for in vitro culture of mammalian embryos. Elamipretide (SS-31) is a synthetic tetrapeptide that binds to the inner mitochondrial membrane, contributing to the prevention of oxidative stress. In this study, the effects of SS-31 supplementation in maturation medium on the developmental competence of porcine oocytes and its protective function were evaluated. Porcine oocytes were matured in maturation medium with SS-31 at 0.001, 0.01, 0.1, 0.5, 1, 1.5, 2.5, and 5 µM, with 0 µM and DMSO-treated groups established as controls. In vitro fertilization and embryo culture were performed to analyze developmental potential. Oocytes cultured in medium with 1 µM SS-31 exhibited higher maturation and blastocyst formation rates than the control (0 µM) (78.3 ± 3.8% vs. 55.2 ± 4.1% and 7.6 ± 1.6% vs. 2.8 ± 1.8%, respectively). Oocytes treated with 1 µM SS-31 showed significantly lower reactive oxygen species, higher glutathione content, and improved mitochondrial membrane potential than those without treatment. DNA fragmentation in oocytes after in vitro maturation was significantly lower in the 1 µM SS-31 supplemented group than in the control. This study demonstrates that SS-31 exerts beneficial effects on the in vitro production of porcine embryos via the enhancement of the mitochondrial antioxidant capacity.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In porcine oocytes, 1 µM SS‑31 in maturation medium increased the maturation rate compared with the 0 µM control.

    In plain wordsIn lab tests on pig eggs, SS‑31 raised the share of eggs that matured compared with no SS‑31.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseOocyte maturation rate
    Study

    pign: not reported in source

    Porcine oocytes, in vitro maturation and fertilization
    Comparator
    Control (0 µM)
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    1 µM · added to maturation mediumAs given in this study (pig).

    Measurements

    Maturation rate
    78.3%
    1 µM SS-31
    Maturation rate
    55.2%
    control 0 µM

    Source sentences

    Summary
    Oocytes cultured in medium with 1 µM SS-31 exhibited higher maturation and blastocyst formation rates than the control (0 µM) (78.3 ± 3.8% vs. 55.2 ± 4.1% and 7.6 ± 1.6% vs. 2.8 ± 1.8%, respectively).
    2 context sentences
    Summary · context
    In this study, the effects of SS-31 supplementation in maturation medium on the developmental competence of porcine oocytes and its protective function were evaluated.
    Summary · context
    Porcine oocytes were matured in maturation medium with SS-31 at 0.001, 0.01, 0.1, 0.5, 1, 1.5, 2.5, and 5 µM, with 0 µM and DMSO-treated groups established as controls.
  2. 02LabIn vitro (cells)Efficacy outcome

    In porcine oocytes, 1 µM SS‑31 in maturation medium increased the blastocyst formation rate compared with the 0 µM control.

    In plain wordsIn lab tests on pig eggs, SS‑31 raised the share of embryos reaching the blastocyst stage compared with no SS‑31.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseBlastocyst formation rate
    Study

    pign: not reported in source

    Porcine oocytes, in vitro maturation and fertilization
    Comparator
    Control (0 µM)
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    1 µM · added to maturation mediumAs given in this study (pig).

    Measurements

    Blastocyst formation rate
    7.6%
    1 µM SS-31
    Blastocyst formation rate
    2.8%
    control 0 µM

    Source sentences

    Summary
    Oocytes cultured in medium with 1 µM SS-31 exhibited higher maturation and blastocyst formation rates than the control (0 µM) (78.3 ± 3.8% vs. 55.2 ± 4.1% and 7.6 ± 1.6% vs. 2.8 ± 1.8%, respectively).
    2 context sentences
    Summary · context
    In this study, the effects of SS-31 supplementation in maturation medium on the developmental competence of porcine oocytes and its protective function were evaluated.
    Summary · context
    Porcine oocytes were matured in maturation medium with SS-31 at 0.001, 0.01, 0.1, 0.5, 1, 1.5, 2.5, and 5 µM, with 0 µM and DMSO-treated groups established as controls.
  3. 03LabIn vitro (cells)Mechanism

    In porcine oocytes, 1 µM SS‑31 lowered reactive oxygen species compared with untreated oocytes.

    In plain wordsIn lab tests on pig eggs, SS‑31 lowered harmful reactive oxygen molecules compared with no SS‑31.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseReactive oxygen species in oocytes
    Study

    pign: not reported in source

    Porcine oocytes, in vitro maturation and fertilization
    Comparator
    Untreated oocytes
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    1 µM · added to maturation mediumAs given in this study (pig).

    Source sentences

    Summary
    Oocytes treated with 1 µM SS-31 showed significantly lower reactive oxygen species, higher glutathione content, and improved mitochondrial membrane potential than those without treatment.
    2 context sentences
    Summary · context
    In this study, the effects of SS-31 supplementation in maturation medium on the developmental competence of porcine oocytes and its protective function were evaluated.
    Summary · context
    Porcine oocytes were matured in maturation medium with SS-31 at 0.001, 0.01, 0.1, 0.5, 1, 1.5, 2.5, and 5 µM, with 0 µM and DMSO-treated groups established as controls.
  4. 04LabIn vitro (cells)Mechanism

    In porcine oocytes, 1 µM SS‑31 increased glutathione content compared with untreated oocytes.

    In plain wordsIn lab tests on pig eggs, SS‑31 raised an antioxidant molecule, glutathione, compared with no SS‑31.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseGlutathione content in oocytes
    Study

    pign: not reported in source

    Porcine oocytes, in vitro maturation and fertilization
    Comparator
    Untreated oocytes
    Outcome group
    Oxidative stress · Reproductive and sexual health
    Studied dose
    1 µM · added to maturation mediumAs given in this study (pig).

    Source sentences

    Summary
    Oocytes treated with 1 µM SS-31 showed significantly lower reactive oxygen species, higher glutathione content, and improved mitochondrial membrane potential than those without treatment.
    2 context sentences
    Summary · context
    In this study, the effects of SS-31 supplementation in maturation medium on the developmental competence of porcine oocytes and its protective function were evaluated.
    Summary · context
    Porcine oocytes were matured in maturation medium with SS-31 at 0.001, 0.01, 0.1, 0.5, 1, 1.5, 2.5, and 5 µM, with 0 µM and DMSO-treated groups established as controls.
  5. 05LabIn vitro (cells)Mechanism

    In porcine oocytes, 1 µM SS‑31 improved mitochondrial membrane potential compared with untreated oocytes.

    In plain wordsIn lab tests on pig eggs, SS‑31 improved mitochondrial function compared with no SS‑31.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseMitochondrial membrane potential in oocytes
    Study

    pign: not reported in source

    Porcine oocytes, in vitro maturation and fertilization
    Comparator
    Untreated oocytes
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    1 µM · added to maturation mediumAs given in this study (pig).

    Source sentences

    Summary
    Oocytes treated with 1 µM SS-31 showed significantly lower reactive oxygen species, higher glutathione content, and improved mitochondrial membrane potential than those without treatment.
    2 context sentences
    Summary · context
    In this study, the effects of SS-31 supplementation in maturation medium on the developmental competence of porcine oocytes and its protective function were evaluated.
    Summary · context
    Porcine oocytes were matured in maturation medium with SS-31 at 0.001, 0.01, 0.1, 0.5, 1, 1.5, 2.5, and 5 µM, with 0 µM and DMSO-treated groups established as controls.
  6. 06LabIn vitro (cells)Mechanism

    In porcine oocytes, 1 µM SS‑31 significantly lowered DNA fragmentation after in vitro maturation compared with the control.

    In plain wordsIn lab tests on pig eggs, SS‑31 lowered DNA damage compared with the control.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseDNA fragmentation in oocytes after maturation
    Study

    pign: not reported in source

    Porcine oocytes, in vitro maturation and fertilization
    Comparator
    Control (0 µM)
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    1 µM · added to maturation mediumAs given in this study (pig).

    Source sentences

    Summary
    DNA fragmentation in oocytes after in vitro maturation was significantly lower in the 1 µM SS-31 supplemented group than in the control.
    2 context sentences
    Summary · context
    In this study, the effects of SS-31 supplementation in maturation medium on the developmental competence of porcine oocytes and its protective function were evaluated.
    Summary · context
    Porcine oocytes were matured in maturation medium with SS-31 at 0.001, 0.01, 0.1, 0.5, 1, 1.5, 2.5, and 5 µM, with 0 µM and DMSO-treated groups established as controls.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 08:19 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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