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Study · Animal study (in vivo) · daphnia · 2025

6 results

Beta-Hydroxybutyrate but not NMN supplementation mimics caloric restriction reducing early mortality in Daphnia.

Pearson AC, Yampolsky LY

Biogerontology25 Aug 2025

PMID40853391 (opens in a new tab)DOI10.1007/s10522-025-10313-z (opens in a new tab)PepBankPUB-40853391
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In fully fed Daphnia, NMN did not reduce early life mortality as BHB did.

    In plain wordsIn Daphnia, NMN did not reduce early mortality, unlike BHB.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeEarly life mortality
    Study

    Daphnian: not reported in source

    fully fed Daphnia
    Outcome group
    Mortality · Aging and longevity
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We show that BHB exposure, but not NMN exposure, reduces early life mortality in fully fed Daphnia to levels similar to those observed under CR without compromising fecundity.
    2 context sentences
    Summary · context
    We tested the hypothesis that chronic BHB and NMN exposure can extend lifespan similarly to the effect of CR treatment in a model organism Daphnia, a freshwater zooplankton crustacean.
    Summary · context
    We also measured fecundity, lipofuscin accumulation, and lipid investments into offspring in Daphnia fed the full diet, full diet with BHB, NMN, and combined treatments, and fed the CR diet (25% of the full diet).
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In Daphnia, adding NMN nearly eliminated the early mortality benefit of BHB.

    In plain wordsIn Daphnia, NMN nearly removed the survival benefit compared with BHB alone.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseBHB mortality benefit
    Study

    Daphnian: not reported in source

    fully fed Daphnia
    Comparator
    BHB alone
    Outcome group
    Mortality · Aging and longevity
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We also observed that in a combined exposure cohort, NMN nearly eliminates the beneficial effect of BHB.
    3 context sentences
    Summary · context
    We show that BHB exposure, but not NMN exposure, reduces early life mortality in fully fed Daphnia to levels similar to those observed under CR without compromising fecundity.
    Summary · context
    We tested the hypothesis that chronic BHB and NMN exposure can extend lifespan similarly to the effect of CR treatment in a model organism Daphnia, a freshwater zooplankton crustacean.
    Summary · context
    We also measured fecundity, lipofuscin accumulation, and lipid investments into offspring in Daphnia fed the full diet, full diet with BHB, NMN, and combined treatments, and fed the CR diet (25% of the full diet).
  3. 03AnimalAnimal study (in vivo)Mechanism

    In Daphnia, NMN did not affect lipofuscin accumulation.

    In plain wordsIn Daphnia, NMN did not change lipofuscin accumulation.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeLipofuscin accumulation
    Study

    Daphnian: not reported in source

    fully fed Daphnia
    Outcome group
    Aging markers · Aging and longevity
    Studied dose
    Dose not stated

    Source sentences

    Summary
    None of the treatments affected lipofuscin accumulation,
    2 context sentences
    Summary · context
    We tested the hypothesis that chronic BHB and NMN exposure can extend lifespan similarly to the effect of CR treatment in a model organism Daphnia, a freshwater zooplankton crustacean.
    Summary · context
    We also measured fecundity, lipofuscin accumulation, and lipid investments into offspring in Daphnia fed the full diet, full diet with BHB, NMN, and combined treatments, and fed the CR diet (25% of the full diet).
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In older female Daphnia, NMN mimicked caloric restriction effects on neonate size.

    In plain wordsIn older female Daphnia, NMN produced neonate size effects similar to caloric restriction.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    Direction not reportedNeonate size
    Study

    Daphnian: not reported in source

    fully fed Daphnia
    Outcome group
    Linear growth · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    but the NMN and the combined treatment mimicked the effect of CR on neonate size in older females.
    2 context sentences
    Summary · context
    We tested the hypothesis that chronic BHB and NMN exposure can extend lifespan similarly to the effect of CR treatment in a model organism Daphnia, a freshwater zooplankton crustacean.
    Summary · context
    We also measured fecundity, lipofuscin accumulation, and lipid investments into offspring in Daphnia fed the full diet, full diet with BHB, NMN, and combined treatments, and fed the CR diet (25% of the full diet).

Studies that did not test the peptide alone2

In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NMN (nicotinamide mononucleotide).

  1. 05AnimalNot a test of NMN (nicotinamide mononucleotide) aloneAnimal study (in vivo)Mechanism

    In Daphnia, combined NMN and BHB did not affect lipofuscin accumulation.

    In plain wordsIn Daphnia, combined NMN and BHB did not change lipofuscin accumulation.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    No significant changeLipofuscin accumulation with combination
    Study

    Daphnian: not reported in source

    fully fed Daphnia
    Outcome group
    Aging markers · Aging and longevity

    Source sentences

    Summary
    None of the treatments affected lipofuscin accumulation,
    2 context sentences
    Summary · context
    We tested the hypothesis that chronic BHB and NMN exposure can extend lifespan similarly to the effect of CR treatment in a model organism Daphnia, a freshwater zooplankton crustacean.
    Summary · context
    We also measured fecundity, lipofuscin accumulation, and lipid investments into offspring in Daphnia fed the full diet, full diet with BHB, NMN, and combined treatments, and fed the CR diet (25% of the full diet).

    In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).

  2. 06AnimalNot a test of NMN (nicotinamide mononucleotide) aloneAnimal study (in vivo)Efficacy outcome

    In older female Daphnia, combined NMN and BHB mimicked caloric restriction effects on neonate size.

    In plain wordsIn older female Daphnia, combined NMN and BHB produced neonate size effects similar to caloric restriction.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    Direction not reportedNeonate size with combination
    Study

    Daphnian: not reported in source

    fully fed Daphnia
    Outcome group
    Linear growth · Whole body

    Source sentences

    Summary
    but the NMN and the combined treatment mimicked the effect of CR on neonate size in older females.
    2 context sentences
    Summary · context
    We tested the hypothesis that chronic BHB and NMN exposure can extend lifespan similarly to the effect of CR treatment in a model organism Daphnia, a freshwater zooplankton crustacean.
    Summary · context
    We also measured fecundity, lipofuscin accumulation, and lipid investments into offspring in Daphnia fed the full diet, full diet with BHB, NMN, and combined treatments, and fed the CR diet (25% of the full diet).

    In this study, NMN (nicotinamide mononucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NMN (nicotinamide mononucleotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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