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Study · Animal study (in vivo) · mouse · 2025

18 results

β-Nicotinamide mononucleotide alleviates Alcohol-Induced liver injury in a mouse model through activation of NAD+/SIRT1 signaling pathways.

Yang X, Zheng E, Lin Y, Sun H, Zhang J, Yu Y

Hereditas16 Aug 2025Cited by 1 (Europe PMC)

PMID40819082 (opens in a new tab)PMCIDPMC12357478 (opens in a new tab)DOI10.1186/s41065-025-00529-x (opens in a new tab)PepBankPUB-40819082
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Summary

BackgroundAlcoholism is a significant contributor to the development of alcoholic liver disease, for which no universally accepted and effective treatment currently exists. A precursor of NAD+, β-Nicotinamide mononucleotide (NMN), has revealed potential therapeutic benefits. However, its effectiveness in preventing ethanol-induced liver damage remains uncertain.MethodsThe objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding. Eight-week-old C57BL/6J mice were randomly assigned to one of four groups (n = 10 per group): control (CTRL), ethanol (EtOH), ethanol with low-dose NMN (EtOH + NMN(L)), and ethanol with high-dose NMN (EtOH + NMN(H)). Following the completion of the experimental protocol, the mice were euthanized at designated time points, and blood, liver, and ileum tissues were collected for analysis of relevant biomarkers.ResultsCompared to the CTRL group, the EtOH group demonstrated increased liver specific gravity and elevated blood ALT levels. Administration of NMN improved histopathological changes in the liver and ileum of the mice. NMN significantly counteracted the ethanol-induced elevation in liver MDA levels and restored the diminished glutathione (GSH) and superoxide dismutase (SOD) activity levels caused by ethanol exposure. Additionally, NMN inhibited the ethanol-induced expression of cytochrome P450 2E1 (CYP2E1). It also reduced the release of pro-inflammatory cytokines, including TNF-α, IL-6, and IL-1β, which were triggered by ethanol exposure, improved energy homeostasis in the ileum, and reversed the downregulation of mRNA and protein expression of key tight junction proteins in the ileum, specifically ZO-1, Claudin-1, and Occludin, thereby restoring their functional integrity. Furthermore, NMN activated the NAD+/ SIRT1 signaling pathway, leading to the upregulation of all target genes.ConclusionNMN supplementation provides protection against alcoholic liver injury in a mouse model, potentially through the upregulation of the cellular NAD+/ SIRT1 pathway. This upregulation enhances antioxidant and anti-inflammatory activities and improves intestinal permeability.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study18

18 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In ethanol-fed mice, NMN improved liver histopathology.

    In plain wordsIn mice exposed to ethanol, NMN improved liver tissue changes.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    Direction not reportedLiver histopathology
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Liver fat and liver function · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Administration of NMN improved histopathological changes in the liver and ileum of the mice.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In ethanol-fed mice, NMN improved ileum histopathology.

    In plain wordsIn mice exposed to ethanol, NMN improved ileum tissue changes.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    Direction not reportedIleum histopathology
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Administration of NMN improved histopathological changes in the liver and ileum of the mice.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN counteracted increased liver MDA.

    In plain wordsIn mice exposed to ethanol, NMN reduced elevated liver MDA.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseLiver MDA
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Oxidative stress · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NMN significantly counteracted the ethanol-induced elevation in liver MDA levels and restored the diminished glutathione (GSH) and superoxide dismutase (SOD) activity levels caused by ethanol exposure.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN restored diminished GSH levels.

    In plain wordsIn mice exposed to ethanol, NMN restored reduced glutathione levels.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseGlutathione
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Oxidative stress · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NMN significantly counteracted the ethanol-induced elevation in liver MDA levels and restored the diminished glutathione (GSH) and superoxide dismutase (SOD) activity levels caused by ethanol exposure.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN restored diminished SOD activity.

    In plain wordsIn mice exposed to ethanol, NMN restored reduced SOD activity.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseSOD activity
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Oxidative stress · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NMN significantly counteracted the ethanol-induced elevation in liver MDA levels and restored the diminished glutathione (GSH) and superoxide dismutase (SOD) activity levels caused by ethanol exposure.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN inhibited ethanol-induced CYP2E1 expression.

    In plain wordsIn mice, NMN reduced CYP2E1 expression induced by ethanol.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseCYP2E1 expression
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Receptor activity and mechanism · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Additionally, NMN inhibited the ethanol-induced expression of cytochrome P450 2E1 (CYP2E1).
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reduced TNF-α release.

    In plain wordsIn mice exposed to ethanol, NMN reduced TNF-α release.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseTNF-α release
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It also reduced the release of pro-inflammatory cytokines, including TNF-α, IL-6, and IL-1β, which were triggered by ethanol exposure,
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reduced IL-6 release.

    In plain wordsIn mice exposed to ethanol, NMN reduced IL-6 release.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseIL-6 release
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It also reduced the release of pro-inflammatory cytokines, including TNF-α, IL-6, and IL-1β, which were triggered by ethanol exposure,
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  9. 09AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reduced IL-1β release.

    In plain wordsIn mice exposed to ethanol, NMN reduced IL-1β release.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    DecreaseIL-1β release
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    It also reduced the release of pro-inflammatory cytokines, including TNF-α, IL-6, and IL-1β, which were triggered by ethanol exposure,
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  10. 10AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN improved ileal energy homeostasis.

    In plain wordsIn mice exposed to ethanol, NMN improved energy balance in the ileum.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    Direction not reportedIleal energy homeostasis
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Mitochondrial function · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    improved energy homeostasis in the ileum,
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  11. 11AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reversed reduced ileal ZO-1 protein expression.

    In plain wordsIn mice exposed to ethanol, NMN restored reduced ileal ZO-1 protein expression.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseZO-1 protein expression
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    and reversed the downregulation of mRNA and protein expression of key tight junction proteins in the ileum, specifically ZO-1, Claudin-1, and Occludin, thereby restoring their functional integrity.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  12. 12AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reversed reduced ileal Claudin-1 protein expression.

    In plain wordsIn mice exposed to ethanol, NMN restored reduced ileal Claudin-1 protein expression.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseClaudin-1 protein expression
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    and reversed the downregulation of mRNA and protein expression of key tight junction proteins in the ileum, specifically ZO-1, Claudin-1, and Occludin, thereby restoring their functional integrity.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  13. 13AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reversed reduced ileal Occludin protein expression.

    In plain wordsIn mice exposed to ethanol, NMN restored reduced ileal Occludin protein expression.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseOccludin protein expression
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    and reversed the downregulation of mRNA and protein expression of key tight junction proteins in the ileum, specifically ZO-1, Claudin-1, and Occludin, thereby restoring their functional integrity.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  14. 14AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN activated NAD+/SIRT1 signaling.

    In plain wordsIn mice exposed to ethanol, NMN activated NAD+/SIRT1 signaling.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseNAD+/SIRT1 signaling
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Receptor activity and mechanism · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Furthermore, NMN activated the NAD+/ SIRT1 signaling pathway, leading to the upregulation of all target genes.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  15. 15AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reversed reduced ileal ZO-1 mRNA expression.

    In plain wordsIn mice exposed to ethanol, NMN restored reduced ileal ZO-1 mRNA expression.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseZO-1 mRNA expression
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    and reversed the downregulation of mRNA and protein expression of key tight junction proteins in the ileum, specifically ZO-1, Claudin-1, and Occludin, thereby restoring their functional integrity.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  16. 16AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reversed reduced ileal Claudin-1 mRNA expression.

    In plain wordsIn mice exposed to ethanol, NMN restored reduced ileal Claudin-1 mRNA expression.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseClaudin-1 mRNA expression
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    and reversed the downregulation of mRNA and protein expression of key tight junction proteins in the ileum, specifically ZO-1, Claudin-1, and Occludin, thereby restoring their functional integrity.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  17. 17AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN reversed reduced ileal Occludin mRNA expression.

    In plain wordsIn mice exposed to ethanol, NMN restored reduced ileal Occludin mRNA expression.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseOccludin mRNA expression
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Gastrointestinal protection · Gastrointestinal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    and reversed the downregulation of mRNA and protein expression of key tight junction proteins in the ileum, specifically ZO-1, Claudin-1, and Occludin, thereby restoring their functional integrity.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.
  18. 18AnimalAnimal study (in vivo)Mechanism

    In ethanol-fed mice, NMN upregulated all target genes of NAD+/SIRT1 signaling.

    In plain wordsIn mice exposed to ethanol, NMN increased target gene expression of NAD+/SIRT1 signaling.

    Peptide
    NMN (nicotinamide mononucleotide)
    Outcome
    IncreaseNAD+/SIRT1 target gene expression
    Study

    mousen: not reported in source

    chronic and binge ethanol feeding
    Outcome group
    Receptor activity and mechanism · Liver
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Furthermore, NMN activated the NAD+/ SIRT1 signaling pathway, leading to the upregulation of all target genes.
    1 context sentence
    Summary · context
    The objective of this study was to assess the protective effects of NMN and elucidate its potential mechanisms using a mouse model subjected to chronic and binge ethanol feeding.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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