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Study · Animal study (in vivo) · mouse · 2025

14 results

S14G-Humanin ameliorates ovarian dysfunction in a cyclophosphamide-induced premature ovarian insufficiency mouse model.

Huang J, Zhang D, Zou L, Liu H, Xia W, Zhu C, Rao M

Molecular human reproduction1 Jul 2025Cited by 1 (Europe PMC)

PMID40811024 (opens in a new tab)DOI10.1093/molehr/gaaf042 (opens in a new tab)PepBankPUB-40811024
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study14

14 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced premature ovarian insufficiency, HNG increased the number of primordial follicles (P = 0.044).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) increased the number of early follicles.

    Peptide
    Humanin
    Outcome
    IncreasePrimordial follicle number
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG administration significantly increased the number of primordial follicles (P = 0.044)
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced premature ovarian insufficiency, the change in the number of growing follicles after HNG administration was not significant (P > 0.05).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) did not clearly change the number of growing follicles.

    Peptide
    Humanin
    Outcome
    No significant changeGrowing follicle number
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    growing follicles (all P > 0.05), as well as corpora lutea (P = 0.09).
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced premature ovarian insufficiency, the change in the number of corpora lutea after HNG administration was not significant (P = 0.09).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) did not clearly change the number of corpora lutea.

    Peptide
    Humanin
    Outcome
    No significant changeCorpora lutea number
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    as well as corpora lutea (P = 0.09).
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  4. 04AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced premature ovarian insufficiency, HNG improved oocyte quality (P = 0.009).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) improved egg quality.

    Peptide
    Humanin
    Outcome
    IncreaseOocyte quality
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG markedly improved oocyte quality (P = 0.009), significantly lowering the proportion of abnormal ovulated oocytes (P = 0.002).
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  5. 05AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced premature ovarian insufficiency, HNG lowered the proportion of abnormal ovulated oocytes (P = 0.002).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) lowered the share of abnormal eggs.

    Peptide
    Humanin
    Outcome
    DecreaseProportion of abnormal ovulated oocytes
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    significantly lowering the proportion of abnormal ovulated oocytes (P = 0.002).
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  6. 06AnimalAnimal study (in vivo)Efficacy outcome

    In mice with cyclophosphamide-induced premature ovarian insufficiency, HNG treatment mitigated the cyclophosphamide-induced reduction in litter size (P = 0.029).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) partly reversed the drop in litter size.

    Peptide
    Humanin
    Outcome
    IncreaseLitter size
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only (litter size 4.6 +/- 1.1)
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    Dose not stated

    Measurements

    litter size
    6.2 NA
    CP + HNG
    litter size
    4.6 NA
    CP

    Source sentences

    Summary
    whereas HNG treatment significantly mitigated this reduction (6.2 ± 0.8 vs 4.6 ± 1.1; P = 0.029).
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced premature ovarian insufficiency, HNG alleviated oxidative stress in ovarian tissues (P < 0.05).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) reduced oxidative stress in the ovary.

    Peptide
    Humanin
    Outcome
    DecreaseOxidative stress in ovarian tissue
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Organ injury · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG alleviated oxidative stress and apoptosis in ovarian tissues (all P < 0.05), reduced ROS levels (P = 0.034), and restored mitochondrial membrane potential (P = 0.004) in a human granulosa cell line.
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced premature ovarian insufficiency, HNG alleviated apoptosis in ovarian tissues (P < 0.05).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) reduced cell death in the ovary.

    Peptide
    Humanin
    Outcome
    DecreaseApoptosis in ovarian tissue
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Organ injury · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG alleviated oxidative stress and apoptosis in ovarian tissues (all P < 0.05), reduced ROS levels (P = 0.034), and restored mitochondrial membrane potential (P = 0.004) in a human granulosa cell line.
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  9. 09AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced premature ovarian insufficiency, HNG upregulated PGC-1α expression (P < 0.05).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) raised PGC-1α, a mitochondrial regulator.

    Peptide
    Humanin
    Outcome
    IncreasePGC-1α expression in vivo
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG significantly upregulated PGC-1α expression and enhanced AMPK phosphorylation in both in vivo and in vitro models (both P < 0.05).
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  10. 10AnimalAnimal study (in vivo)Mechanism

    In mice with cyclophosphamide-induced premature ovarian insufficiency, HNG enhanced AMPK phosphorylation (P < 0.05).

    In plain wordsIn mice with chemotherapy-induced ovarian damage, a Humanin analogue (HNG) increased activation of the AMPK energy sensor.

    Peptide
    Humanin
    Outcome
    IncreaseAMPK phosphorylation in vivo
    Study

    mousen: not reported in source

    cyclophosphamide-induced premature ovarian insufficiency
    Comparator
    Cyclophosphamide only
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    enhanced AMPK phosphorylation in both in vivo and in vitro models (both P < 0.05).
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  11. 11LabIn vitro (cells)Mechanism

    In a human granulosa cell line, HNG reduced ROS levels (P = 0.034).

    In plain wordsIn cells, a Humanin analogue (HNG) lowered reactive oxygen species.

    Peptide
    Humanin
    Outcome
    DecreaseROS levels
    Study

    humann: not reported in source

    human granulosa cell line
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    reduced ROS levels (P = 0.034), and restored mitochondrial membrane potential (P = 0.004) in a human granulosa cell line
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  12. 12LabIn vitro (cells)Mechanism

    In a human granulosa cell line, HNG restored mitochondrial membrane potential (P = 0.004).

    In plain wordsIn cells, a Humanin analogue (HNG) restored the electrical charge across mitochondrial membranes.

    Peptide
    Humanin
    Outcome
    IncreaseMitochondrial membrane potential
    Study

    humann: not reported in source

    human granulosa cell line
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    restored mitochondrial membrane potential (P = 0.004) in a human granulosa cell line
    1 context sentence
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  13. 13LabIn vitro (cells)Mechanism

    In a human granulosa cell line, HNG upregulated PGC-1α expression (P < 0.05).

    In plain wordsIn cells, a Humanin analogue (HNG) raised PGC-1α, a mitochondrial regulator.

    Peptide
    Humanin
    Outcome
    IncreasePGC-1α expression in vitro
    Study

    humann: not reported in source

    human granulosa cell line
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HNG significantly upregulated PGC-1α expression and enhanced AMPK phosphorylation in both in vivo and in vitro models (both P < 0.05).
    2 context sentences
    Summary · context
    human granulosa cell line.
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.
  14. 14LabIn vitro (cells)Mechanism

    In a human granulosa cell line, HNG enhanced AMPK phosphorylation (P < 0.05).

    In plain wordsIn cells, a Humanin analogue (HNG) increased activation of the AMPK energy sensor.

    Peptide
    Humanin
    Outcome
    IncreaseAMPK phosphorylation in vitro
    Study

    humann: not reported in source

    human granulosa cell line
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    Dose not stated

    Source sentences

    Summary
    enhanced AMPK phosphorylation in both in vivo and in vitro models (both P < 0.05).
    2 context sentences
    Summary · context
    human granulosa cell line.
    Summary · context
    In this study, a cyclophosphamide (CP)-induced POI mouse model was established to evaluate both the ovarian damage induced by chemotherapy and the protective effects of HNG.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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