The Therapeutic Effects of Purified Cortrophin Gel on Experimental Autoimmune Uveitis.

Taylor AW, Kim M, Zhao JL, Ng TF

Ocular immunology and inflammation22 Jul 2025

PMID40694652PMCIDPMC12313274DOI10.1080/09273948.2025.2532821PepBankPUB-40694652

Summary

PurposeThe melanocortin pathways are central in maintaining the normal anti-inflammatory microenvironment of the eye. A repository corticotropin injection (RCI) from ANI Pharmaceuticals that activates multiple melanocortin pathways was studied for its effects on experimental autoimmune uveitis (EAU), a murine model of human endogenous uveitis.MethodsAt the chronic phase of EAU, the mice received an injection of the RCI. Clinical scoring of the eyes was conducted every 3 to 4 days using fundus microscopy until the uveitis resolved. The eyes were collected, sectioned, and H&E stained for histological scoring. A T-cell reaction assay was performed using spleen cells to measure IFN-g, IL-17, and IL-10 following restimulation with retinal antigen. The effects of the RCI's active pharmaceutical ingredient (API) on LPS-stimulated macrophage production of IL-1β, IL-10, and TNF-α were assessed by ELISA.ResultThe RCI in a dose-dependent manner suppressed the clinical scores of EAU, showing no significant difference in scoring between 400 U/kg of RCI and a group of native α-MSH-treated mice. In parallel, the retinas of the treated mice had a significant decrease in the histology scores. A significant reduction in IFN-γ and IL-17 production was observed in spleen T-cells of the treated EAU mice, with no regulatory immunity to retinal antigens. The API significantly suppressed the production of IL-1β, IL-10, and TNF-α by stimulated macrophages.ConclusionsRCI treatment effectively suppressed uveitis and protected the retina from inflammatory damage. These findings further illustrate the potential of melanocortin-based therapies for uveitis.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In mice with EAU, Purified Cortrophin Gel suppressed clinical scores in a dose-dependent manner.
In plain wordsIn mice, Purified Cortrophin Gel reduced eye inflammation scores in a dose-dependent manner.
Peptide
ACTH (corticotropin)
Outcome
DecreaseClinical EAU score
Study
Mousen: not reported in source
Experimental autoimmune uveitis
Outcome group
Inflammation markers · Eye
Studied dose
Dose not stated
Source sentences
Summary
The RCI in a dose-dependent manner suppressed the clinical scores of EAU, showing no significant difference in scoring between 400 U/kg of RCI and a group of native α-MSH-treated mice.
1 context sentence(design, population, supporting numbers)
Summary · context
A repository corticotropin injection (RCI) from ANI Pharmaceuticals that activates multiple melanocortin pathways was studied for its effects on experimental autoimmune uveitis (EAU), a murine model of human endogenous uveitis.MethodsAt the chronic phase of EAU, the mice received an injection of the RCI.
Show in the study text Report a problemFND-4069465201
02AnimalAnimal study (in vivo)Efficacy outcome
In mice with EAU, clinical scores did not differ significantly between Purified Cortrophin Gel and native α-MSH.
In plain wordsIn mice, eye scores did not differ significantly between Purified Cortrophin Gel and native α-MSH.
Peptide
ACTH (corticotropin)
Outcome
No significant changeClinical EAU score versus alpha-MSH
Study
Mousen: not reported in source
Experimental autoimmune uveitis
Comparator
Native α-MSH
Outcome group
Inflammation markers · Eye
Studied dose
400 U/kgAs given in this study (Mouse).
Source sentences
Summary
The RCI in a dose-dependent manner suppressed the clinical scores of EAU, showing no significant difference in scoring between 400 U/kg of RCI and a group of native α-MSH-treated mice.
1 context sentence(design, population, supporting numbers)
Summary · context
A repository corticotropin injection (RCI) from ANI Pharmaceuticals that activates multiple melanocortin pathways was studied for its effects on experimental autoimmune uveitis (EAU), a murine model of human endogenous uveitis.MethodsAt the chronic phase of EAU, the mice received an injection of the RCI.
Show in the study text Report a problemFND-4069465202
03AnimalAnimal study (in vivo)Efficacy outcome
In mice with EAU, Purified Cortrophin Gel significantly decreased retinal histology scores.
In plain wordsIn mice, Purified Cortrophin Gel reduced retinal damage scores.
Peptide
ACTH (corticotropin)
Outcome
DecreaseRetinal histology score
Study
Mousen: not reported in source
Experimental autoimmune uveitis
Outcome group
Inflammation markers · Eye
Studied dose
Dose not stated
Source sentences
Summary
In parallel, the retinas of the treated mice had a significant decrease in the histology scores.
1 context sentence(design, population, supporting numbers)
Summary · context
A repository corticotropin injection (RCI) from ANI Pharmaceuticals that activates multiple melanocortin pathways was studied for its effects on experimental autoimmune uveitis (EAU), a murine model of human endogenous uveitis.MethodsAt the chronic phase of EAU, the mice received an injection of the RCI.
Show in the study text Report a problemFND-4069465203
04AnimalAnimal study (in vivo)Mechanism
In treated EAU mice, Purified Cortrophin Gel significantly reduced spleen T-cell IFN-γ production.
In plain wordsIn mice, Purified Cortrophin Gel reduced IFN-γ production by spleen immune cells.
Peptide
ACTH (corticotropin)
Outcome
DecreaseSpleen T-cell IFN-γ production
Study
Mousen: not reported in source
Experimental autoimmune uveitis
Outcome group
Immune response · Eye
Studied dose
Dose not stated
Source sentences
Summary
A significant reduction in IFN-γ and IL-17 production was observed in spleen T-cells of the treated EAU mice, with no regulatory immunity to retinal antigens.
1 context sentence(design, population, supporting numbers)
Summary · context
A repository corticotropin injection (RCI) from ANI Pharmaceuticals that activates multiple melanocortin pathways was studied for its effects on experimental autoimmune uveitis (EAU), a murine model of human endogenous uveitis.MethodsAt the chronic phase of EAU, the mice received an injection of the RCI.
Show in the study text Report a problemFND-4069465204
05AnimalAnimal study (in vivo)Mechanism
In treated EAU mice, Purified Cortrophin Gel significantly reduced spleen T-cell IL-17 production.
In plain wordsIn mice, Purified Cortrophin Gel reduced IL-17 production by spleen immune cells.
Peptide
ACTH (corticotropin)
Outcome
DecreaseSpleen T-cell IL-17 production
Study
Mousen: not reported in source
Experimental autoimmune uveitis
Outcome group
Immune response · Eye
Studied dose
Dose not stated
Source sentences
Summary
A significant reduction in IFN-γ and IL-17 production was observed in spleen T-cells of the treated EAU mice, with no regulatory immunity to retinal antigens.
1 context sentence(design, population, supporting numbers)
Summary · context
A repository corticotropin injection (RCI) from ANI Pharmaceuticals that activates multiple melanocortin pathways was studied for its effects on experimental autoimmune uveitis (EAU), a murine model of human endogenous uveitis.MethodsAt the chronic phase of EAU, the mice received an injection of the RCI.
Show in the study text Report a problemFND-4069465205
06AnimalAnimal study (in vivo)Mechanism
In treated EAU mice, no regulatory immunity to retinal antigens was observed after Purified Cortrophin Gel.
In plain wordsIn mice, no regulatory immunity to retinal antigens was observed after Purified Cortrophin Gel.
Peptide
ACTH (corticotropin)
Outcome
Not applicableRegulatory immunity to retinal antigens
Study
Mousen: not reported in source
Experimental autoimmune uveitis
Outcome group
Immune response · Eye
Studied dose
Dose not stated
Source sentences
Summary
A significant reduction in IFN-γ and IL-17 production was observed in spleen T-cells of the treated EAU mice, with no regulatory immunity to retinal antigens.
1 context sentence(design, population, supporting numbers)
Summary · context
A repository corticotropin injection (RCI) from ANI Pharmaceuticals that activates multiple melanocortin pathways was studied for its effects on experimental autoimmune uveitis (EAU), a murine model of human endogenous uveitis.MethodsAt the chronic phase of EAU, the mice received an injection of the RCI.
Show in the study text Report a problemFND-4069465206

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
28 Oct 2026, 02:03 UTC
Study results updated
Version 1
27 Oct 2026, 16:35 UTC
First added · source update from Europe PMC

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