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Study · In vitro (cells) · 2025

12 results

Targeting ERRs to counteract age-related muscle atrophy associated with physical inactivity: a pilot study.

Bonanni R, Falvino A, Matticari A, Rinaldi AM, D'Arcangelo G, Cifelli P, Iundusi R, Gasbarra E, Tancredi V, Cariati I, Tarantino U

Frontiers in physiology7 Jul 2025Cited by 3 (Europe PMC)

PMID40692696 (opens in a new tab)PMCIDPMC12277287 (opens in a new tab)DOI10.3389/fphys.2025.1616693 (opens in a new tab)PepBankPUB-40692696
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Summary

IntroductionEstrogen-related receptors has been suggested as a potential therapeutic target to counteract muscle decline associated with aging or inactivity, being known to regulate mitochondrial function and cellular respiration by up-regulating key factors in muscle responses to exercise. This study aimed to evaluate the targeting of ERRs in myoblasts isolated from the skeletal muscle of inactive women by assessing the metabolic and expression changes associated with its activation.MethodsTwenty women undergoing hip arthroplasty for coxarthrosis were enrolled and divided into an active group (n = 10) and an inactive group (n = 10) based on self-reported physical activity. During surgery, muscle biopsies were taken for histological and western blotting analysis, measuring the expression levels of NADPH oxidase 4 (NOX4), sirtuin 1 (SIRT1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), estrogen related receptor alpha (ERRα), and fibronectin type III domain-containing protein 5 (FNDC5). Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations. In addition, myoblasts were differentiated into myotubes for 15 days, and the success of differentiation was evaluated by immunofluorescence analysis.ResultsClinical and instrumental evaluation showed less functional limitation, higher handgrip strength values, and significantly reduced visual analogue scale scores in active subjects, in association with a significant increase in muscle fiber diameter. In addition, significantly higher expression of NOX4, concomitant with reduced levels of SIRT1, PGC-1α, ERRα, and FNDC5, was detected in the muscle tissue of inactive women. Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione. Furthermore, SLU-PP-332 treatment promoted abundant myotube formation, positively influencing cell differentiation.DiscussionTargeting ERRs could represent a promising therapeutic strategy to counteract muscle atrophy in elderly and sedentary subjects. However, further studies are needed to clarify the molecular mechanisms involved and explore the impact of ERRs activation on muscle metabolism.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study12

12 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 reduced NOX4 expression.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 reduced NOX4 expression.

    Peptide
    SLU-PP-332
    Outcome
    DecreaseNOX4 expression
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  2. 02LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 increased SIRT1 expression.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 increased SIRT1 expression.

    Peptide
    SLU-PP-332
    Outcome
    IncreaseSIRT1 expression
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  3. 03LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 increased PGC-1α expression.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 increased PGC-1α expression.

    Peptide
    SLU-PP-332
    Outcome
    IncreasePGC-1α expression
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  4. 04LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 increased ERRα expression.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 increased ERRα expression.

    Peptide
    SLU-PP-332
    Outcome
    IncreaseERRα expression
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  5. 05LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 increased FNDC5 expression.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 increased FNDC5 expression.

    Peptide
    SLU-PP-332
    Outcome
    IncreaseFNDC5 expression
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  6. 06LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 increased Akt expression.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 increased Akt expression.

    Peptide
    SLU-PP-332
    Outcome
    IncreaseAkt expression
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  7. 07LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 increased Bcl-2 expression.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 increased Bcl-2 expression.

    Peptide
    SLU-PP-332
    Outcome
    IncreaseBcl-2 expression
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  8. 08LabIn vitro (cells)Efficacy outcome

    In myoblasts from inactive women, SLU‑PP‑332 reduced cytotoxicity.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 reduced cell toxicity.

    Peptide
    SLU-PP-332
    Outcome
    DecreaseCytotoxicity
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Cell viability and death · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  9. 09LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 reduced oxidative stress.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 reduced oxidative stress.

    Peptide
    SLU-PP-332
    Outcome
    DecreaseOxidative stress
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Mitochondrial function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  10. 10LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 reduced senescence.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 reduced cell senescence.

    Peptide
    SLU-PP-332
    Outcome
    DecreaseSenescence
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Aging markers · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  11. 11LabIn vitro (cells)Mechanism

    In myoblasts from inactive women, SLU‑PP‑332 increased reduced glutathione levels.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 increased reduced glutathione levels.

    Peptide
    SLU-PP-332
    Outcome
    IncreaseReduced glutathione levels
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Mitochondrial function · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, SLU-PP-332 treatment promoted down-regulation of NOX4 and upregulation of SIRT1, PGC-1α, ERRα, FNDC5, Akt, and B-cell lymphoma 2 (Bcl-2) in myoblasts, reducing cytotoxicity, oxidative stress, and senescence, as well as increasing levels of reduced glutathione.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.
  12. 12LabIn vitro (cells)Efficacy outcome

    In myoblast cultures from inactive women, SLU‑PP‑332 promoted abundant myotube formation.

    In plain wordsIn cultured myoblasts, SLU‑PP‑332 promoted myotube formation.

    Peptide
    SLU-PP-332
    Outcome
    IncreaseMyotube formation
    Study

    humann: not reported in source

    Primary myoblasts from inactive women
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Furthermore, SLU-PP-332 treatment promoted abundant myotube formation, positively influencing cell differentiation.
    1 context sentence
    Summary · context
    Primary cultures of myoblasts were set up from the muscle tissue of inactive women and treated with the ERRs agonist, SLU-PP-332, for subsequent qualitative and quantitative investigations.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    14 Oct 2026, 05:02 UTC

    Study results updated

  2. Version 1

    14 Oct 2026, 01:46 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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