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Study · Ex vivo tissue · chicken · 2025

7 results

Elamipretide enhances post-thaw rooster sperm quality by mitigating oxidative stress and optimizing mitochondrial function during cryopreservation.

Najafi A, Sharifi SD, Farhadi R, Bapir SH

Scientific reports2 Jul 2025Cited by 5 (Europe PMC)

PMID40604246 (opens in a new tab)PMCIDPMC12223306 (opens in a new tab)DOI10.1038/s41598-025-09943-7 (opens in a new tab)PepBankPUB-40604246
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueEfficacy outcome

    In cryopreserved rooster sperm, elamipretide at 6 and 9 µmol/L increased post-thaw motility versus control.

    In plain wordsIn rooster sperm, elamipretide at 6 and 9 µmol/L improved movement after thawing compared with no elamipretide.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreasePost-thaw sperm motility
    Study

    chickenn: not reported in source

    cryopreserved broiler breeder rooster sperm
    Comparator
    Control (0 µmol/L)
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    6 and 9 µmol/L · added to extenderAs given in this study (chicken).

    Source sentences

    Summary
    Sperm motility increased significantly at the 6 µmol/L (60.33 ± 1.54) and 9 µmol/L (64.96 ± 1.96) concentrations compared to the control
    2 context sentences
    Summary · context
    Sperm samples from 32-week-old broiler breeder roosters were cryopreserved using a Lake extender buffer with glycerol as the cryoprotectant.
    Summary · context
    Four different concentrations of elamipretide (0, 6, 9, and 12 µmol/L) were tested in combination with the extender.
  2. 02LabEx vivo tissueEfficacy outcome

    In cryopreserved rooster sperm, the highest straight-line velocity was seen at 9 µmol/L elamipretide.

    In plain wordsIn rooster sperm, the highest swimming speed after thawing was with elamipretide at 9 µmol/L.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseStraight-line velocity
    Study

    chickenn: not reported in source

    cryopreserved broiler breeder rooster sperm
    Comparator
    Other concentrations
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    9 µmol/L · added to extenderAs given in this study (chicken).

    Source sentences

    Summary
    with the highest straight-line velocity observed at 9 µmol/L (21.21 ± 0.59).
    2 context sentences
    Summary · context
    Sperm samples from 32-week-old broiler breeder roosters were cryopreserved using a Lake extender buffer with glycerol as the cryoprotectant.
    Summary · context
    Four different concentrations of elamipretide (0, 6, 9, and 12 µmol/L) were tested in combination with the extender.
  3. 03LabEx vivo tissueEfficacy outcome

    In cryopreserved rooster sperm, membrane integrity improved at 9 µmol/L elamipretide compared with lower doses.

    In plain wordsIn rooster sperm, elamipretide at 9 µmol/L improved membrane integrity compared with lower doses.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseMembrane integrity
    Study

    chickenn: not reported in source

    cryopreserved broiler breeder rooster sperm
    Comparator
    Lower doses
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    9 µmol/L · added to extenderAs given in this study (chicken).

    Source sentences

    Summary
    Membrane integrity also improved significantly at 9 µmol/L (61.78 ± 2.70) compared to lower doses (36.30 ± 1.64)
    2 context sentences
    Summary · context
    Sperm samples from 32-week-old broiler breeder roosters were cryopreserved using a Lake extender buffer with glycerol as the cryoprotectant.
    Summary · context
    Four different concentrations of elamipretide (0, 6, 9, and 12 µmol/L) were tested in combination with the extender.
  4. 04LabEx vivo tissueEfficacy outcome

    In cryopreserved rooster sperm, membrane integrity was lower at 12 µmol/L elamipretide than at 9 µmol/L.

    In plain wordsIn rooster sperm, membrane integrity was lower with 12 µmol/L elamipretide than with 9 µmol/L.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseMembrane integrity at 12 µmol/L
    Study

    chickenn: not reported in source

    cryopreserved broiler breeder rooster sperm
    Comparator
    9 µmol/L
    Outcome group
    Sex hormones and fertility · Reproductive and sexual health
    Studied dose
    12 µmol/L · added to extenderAs given in this study (chicken).

    Source sentences

    Summary
    and decreased at 12 µmol/L (49.57 ± 1.63).
    2 context sentences
    Summary · context
    Sperm samples from 32-week-old broiler breeder roosters were cryopreserved using a Lake extender buffer with glycerol as the cryoprotectant.
    Summary · context
    Four different concentrations of elamipretide (0, 6, 9, and 12 µmol/L) were tested in combination with the extender.
  5. 05LabEx vivo tissueEfficacy outcome

    In cryopreserved rooster sperm, ROS production was significantly lower at 6 µmol/L elamipretide.

    In plain wordsIn rooster sperm, elamipretide at 6 µmol/L lowered reactive oxygen species.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseROS production
    Study

    chickenn: not reported in source

    cryopreserved broiler breeder rooster sperm
    Comparator
    Control (0 µmol/L)
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    6 µmol/L · added to extenderAs given in this study (chicken).

    Source sentences

    Summary
    ROS production was significantly lower at 6 µmol/L (2.88 ± 0.07).
    2 context sentences
    Summary · context
    Sperm samples from 32-week-old broiler breeder roosters were cryopreserved using a Lake extender buffer with glycerol as the cryoprotectant.
    Summary · context
    Four different concentrations of elamipretide (0, 6, 9, and 12 µmol/L) were tested in combination with the extender.
  6. 06LabEx vivo tissueEfficacy outcome

    In cryopreserved rooster sperm, mitochondrial activity peaked at 9 µmol/L elamipretide.

    In plain wordsIn rooster sperm, mitochondrial activity was highest with elamipretide at 9 µmol/L.

    Peptide
    Elamipretide (SS-31)
    Outcome
    IncreaseMitochondrial activity
    Study

    chickenn: not reported in source

    cryopreserved broiler breeder rooster sperm
    Comparator
    Other concentrations
    Outcome group
    Mitochondrial function · Reproductive and sexual health
    Studied dose
    9 µmol/L · added to extenderAs given in this study (chicken).

    Source sentences

    Summary
    Mitochondrial activity peaked at 9 µmol/L (60.21 ± 1.92), reflecting enhanced cell vitality and function.
    2 context sentences
    Summary · context
    Sperm samples from 32-week-old broiler breeder roosters were cryopreserved using a Lake extender buffer with glycerol as the cryoprotectant.
    Summary · context
    Four different concentrations of elamipretide (0, 6, 9, and 12 µmol/L) were tested in combination with the extender.
  7. 07LabEx vivo tissueSafety outcome

    In cryopreserved rooster sperm, the beneficial effects of elamipretide diminished at 12 µmol/L, indicating toxicity at higher concentrations.

    In plain wordsIn rooster sperm, the benefits of elamipretide faded at 12 µmol/L, which suggests toxicity at high concentrations.

    Peptide
    Elamipretide (SS-31)
    Outcome
    DecreaseToxicity at high concentration
    Study

    chickenn: not reported in source

    cryopreserved broiler breeder rooster sperm
    Comparator
    6 and 9 µmol/L
    Outcome group
    Adverse events · Reproductive and sexual health
    Studied dose
    12 µmol/L · added to extenderAs given in this study (chicken).

    Source sentences

    Summary
    However, the effects were diminished at 12 µmol/L, indicating toxicity at higher concentrations.
    2 context sentences
    Summary · context
    Sperm samples from 32-week-old broiler breeder roosters were cryopreserved using a Lake extender buffer with glycerol as the cryoprotectant.
    Summary · context
    Four different concentrations of elamipretide (0, 6, 9, and 12 µmol/L) were tested in combination with the extender.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 08:19 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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