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Study · Measured the body's own peptide · 2025

4 results

Effect of neurokinin B and dynorphin A on kisspeptin-10 secretion from the anterior pituitary cells of pubescent ewes in vitro.

Szysiak N, Kosior-Korzecka U, Greguła-Kania M, Patkowski K, Fila M, Junkuszew A

Journal of veterinary research3 May 2025Cited by 2 (Europe PMC)

PMID40552024 (opens in a new tab)PMCIDPMC12182939 (opens in a new tab)DOI10.2478/jvetres-2025-0026 (opens in a new tab)PepBankPUB-40552024
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Summary

IntroductionNeurokinin B (NKB), dynorphin A (Dyn A) and kisspeptin (KiSS) are key agents that participate in the neuroendocrine regulation of the development and functioning of the reproductive system. While the role of KiSS is better understood, the functions of NKB and Dyn A at the pituitary level have not been elucidated. The objective of our study was to analyse their direct effect on kisspeptin-10 (KiSS-10) secretion by anterior pituitary cells isolated from pubescent ewes.Material and methodsPituitary cells from 10-month-old ewe lambs were incubated in McCoy's 5A medium without hormones (the control), or with 10-11, 10-10, 10-9, 10-8 or 10-7 M of NKB or Dyn A for 2, 4, 6, 12, 18 or 24 h. The concentration of KiSS-10 was analysed by ELISA using species-specific antibodies.ResultsWhen applied at the concentrations of 10-10-10-7 M, NKB increased KiSS-10 secretion throughout the entire experiment (2-24 h), compared to the control. Significantly higher (P-value ≤ 0.05) KiSS-10 release than in the control was observed after 6-24 h exposure of the cells to 10-8 M of NKB. However, no effect of NKB on the secretion of KiSS-10 was shown when applied at the lowest concentration (10-11 M). In turn, there was no significant effect of Dyn A at any concentration on KiSS-10 secretion by pituitary cells at any time.ConclusionIn contrast to Dyn A, NKB can directly affect KiSS-10 secretion from the pituitary cells of pubescent ewes in a way dependent on the time of exposure to this neuropeptide and its concentration in the culture medium. This phenomenon may indicate a potential role of NKB in the initiation of reproductive activity, which leads to the achievement of sexual maturity in the optimal developmental window.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

No result from this study comes from a test of the peptide.

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 01LabEndogenous levelIn vitro (cells)Activity measurement

    In primary pituitary cells from pubescent ewes, neurokinin B increased secretion of the cells' own kisspeptin‑10 throughout 2-24 h compared with the control.

    In plain wordsIn cells from sheep pituitary glands, neurokinin B raised the cells' own kisspeptin‑10 release compared with untreated cells.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseKiSS-10 secretion after neurokinin B
    Study

    sheepn: not reported in source

    Primary anterior pituitary cells from pubescent ewes
    Comparator
    Control (no hormone)
    Outcome group
    Sex hormones and fertility · Endocrine and hormones

    Source sentences

    Summary
    When applied at the concentrations of 10-10-10-7 M, NKB increased KiSS-10 secretion throughout the entire experiment (2-24 h), compared to the control.
    1 context sentence
    Summary · context
    Pituitary cells from 10-month-old ewe lambs were incubated in McCoy's 5A medium without hormones (the control), or with 10-11, 10-10, 10-9, 10-8 or 10-7 M of NKB or Dyn A for 2, 4, 6, 12, 18 or 24 h.

    This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.

  2. 02LabEndogenous levelIn vitro (cells)Activity measurement

    In primary pituitary cells from pubescent ewes, neurokinin B at one tested concentration raised the cells' own kisspeptin‑10 release above the control after 6-24 h.

    In plain wordsIn cells from sheep pituitary glands, neurokinin B at one dose raised the cells' own kisspeptin‑10 release after 6 to 24 hours.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseKiSS-10 secretion after neurokinin B, time course at one concentration
    Study

    sheepn: not reported in source

    Primary anterior pituitary cells from pubescent ewes
    Comparator
    Control (no hormone)
    Outcome group
    Sex hormones and fertility · Endocrine and hormones

    Source sentences

    Summary
    Significantly higher (P-value ≤ 0.05) KiSS-10 release than in the control was observed after 6-24 h exposure of the cells to 10-8 M of NKB.
    1 context sentence
    Summary · context
    Pituitary cells from 10-month-old ewe lambs were incubated in McCoy's 5A medium without hormones (the control), or with 10-11, 10-10, 10-9, 10-8 or 10-7 M of NKB or Dyn A for 2, 4, 6, 12, 18 or 24 h.

    This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.

  3. 03LabEndogenous levelIn vitro (cells)Activity measurement

    In primary pituitary cells from pubescent ewes, neurokinin B at the lowest concentration did not change the cells' own kisspeptin‑10 secretion.

    In plain wordsIn cells from sheep pituitary glands, the lowest neurokinin B dose did not change the cells' own kisspeptin‑10 release.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKiSS-10 secretion after neurokinin B at lowest concentration
    Study

    sheepn: not reported in source

    Primary anterior pituitary cells from pubescent ewes
    Comparator
    Control (no hormone)
    Outcome group
    Sex hormones and fertility · Endocrine and hormones

    Source sentences

    Summary
    However, no effect of NKB on the secretion of KiSS-10 was shown when applied at the lowest concentration (10-11 M).
    1 context sentence
    Summary · context
    Pituitary cells from 10-month-old ewe lambs were incubated in McCoy's 5A medium without hormones (the control), or with 10-11, 10-10, 10-9, 10-8 or 10-7 M of NKB or Dyn A for 2, 4, 6, 12, 18 or 24 h.

    This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.

  4. 04LabEndogenous levelIn vitro (cells)Activity measurement

    In primary pituitary cells from pubescent ewes, dynorphin A at any concentration and time had no significant effect on the cells' own kisspeptin‑10 secretion.

    In plain wordsIn cells from sheep pituitary glands, dynorphin A did not change the cells' own kisspeptin‑10 release at any dose or time.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKiSS-10 secretion after dynorphin A
    Study

    sheepn: not reported in source

    Primary anterior pituitary cells from pubescent ewes
    Comparator
    Control (no hormone)
    Outcome group
    Sex hormones and fertility · Endocrine and hormones

    Source sentences

    Summary
    In turn, there was no significant effect of Dyn A at any concentration on KiSS-10 secretion by pituitary cells at any time.
    1 context sentence
    Summary · context
    Pituitary cells from 10-month-old ewe lambs were incubated in McCoy's 5A medium without hormones (the control), or with 10-11, 10-10, 10-9, 10-8 or 10-7 M of NKB or Dyn A for 2, 4, 6, 12, 18 or 24 h.

    This result measures the body's own Kisspeptin-10. Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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