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Study · Animal study (in vivo) · rat · 2025

10 results

Adult Neurogenesis Is Regulated by the Endocannabinoid and Kisspeptin Systems.

Marino M, Di Pietro P, D'Auria R, Lombardi M, Pastorino GMG, Troisi J, Operto FF, Carrizzo A, Vecchione C, Viggiano A, Meccariello R, Santoro A

International journal of molecular sciences23 Apr 2025Cited by 3 (Europe PMC)

PMID40362219 (opens in a new tab)PMCIDPMC12071241 (opens in a new tab)DOI10.3390/ijms26093977 (opens in a new tab)PepBankPUB-40362219
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Summary

Neurogenesis is considered the most robust form of plasticity in the adult brain. To better decipher this process, we evaluated the potential crosstalk of Kisspeptin and Endocannabinoid Systems (KPS and ECS, respectively) on hippocampal neurogenesis. Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A. The expression of Kiss1 and Kisspeptin receptor (Kiss1R) has been characterized for the first time in rat hippocampus together with the expression of the CB1R and the Transient Receptor Potential Vanilloid 1 ion channel receptor (TRPV1). Results show that both systems inhibit neurogenesis by reducing the extracellular signal-regulated kinase (ERK) signaling. Despite little differences in the expression of Kiss1R and CB1R, TRPV1 is enhanced by both KP10 and AEA treatments, suggesting TRPV1 as a common thread. KP10 administration reduces CB1R expression in the dentate gyrus, while AEA does not. KPS, unlike ECS, promotes the expression of estrogen receptor α (ER-α) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also upregulating sirtuin 1 (SIRT1), brain-derived-neurotrophic factor (BDNF), and c-Jun. These findings suggest that the interaction between ECS and KPS could be involved in the fine-tuning of neurogenesis, highlighting a novel role for KPS.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study10

10 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In male adolescent rats, kisspeptin‑10 and anandamide each inhibited hippocampal neurogenesis.

    In plain wordsIn young male rats, kisspeptin‑10 reduced the formation of new neurons in the hippocampus.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseHippocampal neurogenesis
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Neuroprotection · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results show that both systems inhibit neurogenesis by reducing the extracellular signal-regulated kinase (ERK) signaling.
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, kisspeptin‑10 and anandamide inhibited neurogenesis by reducing extracellular signal-regulated kinase (ERK) signaling.

    In plain wordsIn young male rats, kisspeptin‑10 lowered ERK signaling in the brain, a pathway linked to new neuron formation.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseERK signaling
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Results show that both systems inhibit neurogenesis by reducing the extracellular signal-regulated kinase (ERK) signaling.
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, TRPV1 expression was enhanced by both kisspeptin‑10 and anandamide treatments.

    In plain wordsIn young male rats, kisspeptin‑10 raised levels of the TRPV1 channel in the hippocampus.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseTRPV1 expression
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    TRPV1 is enhanced by both KP10 and AEA treatments, suggesting TRPV1 as a common thread.
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, there were little differences in the expression of Kiss1R and CB1R after treatment.

    In plain wordsIn young male rats, treatment changed the levels of the kisspeptin receptor and the CB1 receptor very little.

    Peptide
    Kisspeptin-10
    Outcome
    No significant changeKiss1R and CB1R expression
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Despite little differences in the expression of Kiss1R and CB1R, TRPV1 is enhanced by both KP10 and AEA treatments, suggesting TRPV1 as a common thread.
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, kisspeptin‑10 reduced CB1R expression in the dentate gyrus, whereas anandamide did not.

    In plain wordsIn young male rats, kisspeptin‑10 lowered CB1 receptor levels in part of the hippocampus; anandamide did not.

    Peptide
    Kisspeptin-10
    Outcome
    DecreaseCB1R expression in dentate gyrus
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    KP10 administration reduces CB1R expression in the dentate gyrus, while AEA does not.
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, the kisspeptin system, unlike the endocannabinoid system, promoted estrogen receptor alpha expression.

    In plain wordsIn young male rats, kisspeptin treatment raised estrogen receptor alpha levels, unlike the endocannabinoid anandamide.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseEstrogen receptor alpha expression
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    KPS, unlike ECS, promotes the expression of estrogen receptor α (ER-α) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, the kisspeptin system, unlike the endocannabinoid system, promoted GAPDH expression.

    In plain wordsIn young male rats, kisspeptin treatment raised GAPDH levels, unlike the endocannabinoid anandamide.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseGAPDH expression
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    KPS, unlike ECS, promotes the expression of estrogen receptor α (ER-α) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, the kisspeptin system, unlike the endocannabinoid system, upregulated sirtuin 1 (SIRT1).

    In plain wordsIn young male rats, kisspeptin treatment raised SIRT1 levels, unlike the endocannabinoid anandamide.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseSIRT1 expression
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    KPS, unlike ECS, promotes the expression of estrogen receptor α (ER-α) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also upregulating sirtuin 1 (SIRT1), brain-derived-neurotrophic factor (BDNF), and c-Jun.
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  9. 09AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, the kisspeptin system, unlike the endocannabinoid system, upregulated brain-derived neurotrophic factor (BDNF).

    In plain wordsIn young male rats, kisspeptin treatment raised BDNF levels, unlike the endocannabinoid anandamide.

    Peptide
    Kisspeptin-10
    Outcome
    IncreaseBDNF expression
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    KPS, unlike ECS, promotes the expression of estrogen receptor α (ER-α) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also upregulating sirtuin 1 (SIRT1), brain-derived-neurotrophic factor (BDNF), and c-Jun.
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.
  10. 10AnimalAnimal study (in vivo)Mechanism

    In male adolescent rats, the kisspeptin system, unlike the endocannabinoid system, upregulated c-Jun.

    In plain wordsIn young male rats, kisspeptin treatment raised c-Jun levels, unlike the endocannabinoid anandamide.

    Peptide
    Kisspeptin-10
    Outcome
    Increasec-Jun expression
    Study

    ratn: not reported in source

    adolescent male rat hippocampus; KP10 and anandamide alone or with CB1R antagonist SR141716A
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    KPS, unlike ECS, promotes the expression of estrogen receptor α (ER-α) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also upregulating sirtuin 1 (SIRT1), brain-derived-neurotrophic factor (BDNF), and c-Jun.
    1 context sentence
    Summary · context
    Male adolescent rats were exposed to kisspeptin-10 (KP10) and the endocannabinoid anandamide (AEA) administered alone or in combination with the type 1 cannabinoid receptor (CB1R) antagonist SR141716A.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

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