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Study · In vitro (cells) · mouse · 2025

2 results

A Dominant Mutation in Gαs-Protein Increases Hair Pigmentation.

Goff PS, Budd P, Logan DW, Keighren M, Cantero M, McKie L, Montoliu L, Jackson IJ, Sviderskaya EV

Pigment cell & melanoma research1 May 2025Cited by 1 (Europe PMC)

PMID40356280 (opens in a new tab)PMCIDPMC12069967 (opens in a new tab)DOI10.1111/pcmr.70025 (opens in a new tab)PepBankPUB-40356280
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Summary

We have identified a chemically induced mouse mutation which increases the eumelanic hair pigmentation. We identify a coding mutation, A3533G, resulting in an amino acid substitution Y1133C, in the Gnas gene encoding the Gαs subunit of the tripartite G-protein, consistent with an activation of signalling via MC1R. In addition heterozygous mutant females are significantly lighter than wild type littermates. In cultured melanocytes, derived from mutant mice crossed to C57BL6 mice carrying Cdkn2atm1Rdp, basal pigmentation is higher than wild type melanocytes derived from litter mates. However, the addition of exogenous NDP-MSH does not increase pigmentation in mutant melanocytes in contrast to the pigmentation response of non-mutant melanocytes. The mutant and wild type cells respond in the same way to agouti signalling protein (ASP), consistent with ASP signalling mediated through a pathway other than Gαs-protein.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In cultured melanocytes from Gnas mutant mice, exogenous NDP-MSH did not increase pigmentation.

    In plain wordsIn cultured mouse pigment cells with a Gnas mutation, NDP-MSH did not darken the cells.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changePigmentation response to NDP-MSH
    Study

    mousen: not reported in source

    melanocytes from Gnas Y1133C mutant mice
    Comparator
    Non-mutant melanocytes
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    the addition of exogenous NDP-MSH does not increase pigmentation in mutant melanocytes
  2. 02LabIn vitro (cells)Efficacy outcome

    In cultured non-mutant mouse melanocytes, exogenous NDP-MSH increased pigmentation, in contrast to mutant melanocytes.

    In plain wordsIn cultured mouse pigment cells without the mutation, NDP-MSH darkened the cells.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasePigmentation response to NDP-MSH
    Study

    mousen: not reported in source

    melanocytes from non-mutant (wild type) littermates
    Comparator
    Mutant melanocytes
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, the addition of exogenous NDP-MSH does not increase pigmentation in mutant melanocytes in contrast to the pigmentation response of non-mutant melanocytes.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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